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All Journal Indonesian Journal of Jamu
Berna Elya
Department of Phytochemistry and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, 16424
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Medicine & Pharmacology

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Ultrasound-Assisted Extraction Enhances Phenolic, Flavonoid, and Antioxidant Profiles of Spatholobus littoralis Roots Muchammad Reza Ghozaly; Berna Elya; Muhammad Hanafi; Heri Setiawan
Jurnal Jamu Indonesia Vol. 11 No. 3 (2026): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v11i3.501

Abstract

Spatholobus littoralis Hassk. roots contain antioxidant-related compounds whose recovery may be influenced by extraction method and solvent polarity. This study compared ultrasound-assisted extraction and microwave-assisted extraction using n-hexane, ethyl acetate, and 96% ethanol to evaluate their effects on total phenolic content, total flavonoid content, and DPPH radical-scavenging activity. Among the tested conditions, 96% ethanol combined with ultrasound-assisted extraction produced the highest phenolic content, flavonoid content, and antioxidant activity, with values of 89.33 mg GAE/g, 40.10 mg QE/g, and IC₅₀ 72.27 µg/mL, respectively. Strong negative correlations were observed between DPPH IC₅₀ and both total phenolic content (r = −0.961) and total flavonoid content (r = −0.941). These findings indicate that ultrasound-assisted extraction with 96% ethanol was the most effective condition for obtaining antioxidant-rich S. littoralis root extract in this study.
Co-Authors Heri Setiawan Muchammad Reza Ghozaly Muhammad Hanafi