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All Journal MARSEGU : Jurnal Sains dan Teknologi
Johan M. Matinahoru
Program Studi Kehutanan, Fakultas Pertanian, Universitas Pattimura, Ambon. Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Engineering

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PENGARUH BAKTERI ENDOFIT TANAMAN SAMAMA (Anthocephallus macropyllus) TERHADAP PERTAMBAHAN TINGGI DAN JUMLAH DAUN SEMAI TANAMAN GANDARIA (Bouea macrophylla) Muhammad Gofal Yahya Tuharea; Johan M. Matinahoru; Fransina S. Latumahina
MARSEGU : Jurnal Sains dan Teknologi Vol. 3 No. 2 (2026): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/3.2.2026.171-192

Abstract

Samama (Anthocephalus macrophyllus) and Gandaria (Bouea macrophylla) are plant species with significant ecological value. Seed germination success is a critical factor in plant nursery development. One approach to enhance germination is through the utilization of endophytic bacteria residing within plant tissues. This study aimed to determine the effect of endophytic bacteria derived from Samama on Gandaria seed germination. A Completely Randomized Design (CRD) was employed with two treatments without fertilizer (A0) and endophytic biofertilizer (A1) each with 10 replications (n = 20). Endophytic bacteria were isolated from Samama roots via 14-day fermentation and applied at a dose of 100 ml per plant to Gandaria seedlings. Observed parameters included plant height, leaf number, and bacterial identification on roots, analyzed using ANOVA and binomial test (Minitab 19). The results showed that endophytic bacteria from Samama, predominantly of the genus Bacillus, were successfully inoculated into Gandaria seedlings, increasing average plant height by 5 cm and leaf count by 4 leaves over two months. Bacterial identification in Gandaria seedlings further revealed the presence of two genera, namely Pseudomonas and Azospirillum.
KERAGAMAN FENOTIPE TANAMAN KENARI (Canarium sp.) PADA KEBUN KOLEKSI DI DESA HATUSUA KECAMATAN KAIRATU KABUPATEN SERAM BAGIAN BARAT Elva Dasilvia Rianekuay; Johan M. Matinahoru; Andjela Sahupala
MARSEGU : Jurnal Sains dan Teknologi Vol. 3 No. 1 (2026): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/3.1.2026.16-46

Abstract

This study aims to analyze the phenotypic diversity of canary plants (Canarium sp.) from three provenances, namely Ambon, Saparua, and Seram, and to observe the relationship between environmental factors and the growth of canary plants in the Canary Collection Garden owned by the Maluku Provincial Forestry Service, located in Hatusua Village, Kairatu District, West Seram Regency. This study used a descriptive method and a completely randomized design (CRD) with ANOVA analysis, multiple regression, and cluster analysis to evaluate phenotypic characters (leaves, stems, fruits/seeds) and environmental factors (light, temperature, humidity, soil pH, fertility, and soil moisture). The results showed that there were significant differences in leaf phenotypic characters (area, length, width), and most of the differences in stem and fruit/seed size were caused by environmental factors. Seram provenance showed greater leaf area and seed weight than Ambon and Saparua. However, the ANOVA results showed that only leaf area showed a significant effect of provenance treatment. Meanwhile, the relationship between vegetative growth (number of branches, stem diameter, and branch-free height) and environmental factors showed varying correlations, depending on the provenance. Cluster analysis grouped the Ambon and Saparua provenances into a phenotypically similar group, while Seram formed a separate cluster.
PEMBIAKAN MIKORIZA ASAL AKAR MERANTI (Shorea montigena) DENGAN MENGGUNAKAN CARA LARUTAN Nuel Patrik Radjabaycolle Bunga; Johan M. Matinahoru; Miranda H. Hadijah
MARSEGU : Jurnal Sains dan Teknologi Vol. 3 No. 1 (2026): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/3.1.2026.47-63

Abstract

Meranti (Shorea montigena Slooten) has roots that serve as a habitat for mycorrhizal fungi. These mycorrhizal fungi can be propagated and transmitted to other plant species. Transmission of mycorrhizal fungi can occur in the roots of host plants, if infection has occurred. So far, the process of breeding endomycorrhizal fungi is mostly done in a non-solution way, so this study aims to find an effective solution medium for breeding endomycorrhizal fungi. The method used is the solution method. The research design used was a single factor experiment in a completely randomized design. The single factor is the type of solution media consisting of 5 levels. The treatment was repeated 3 times and each treatment consisted of 5 corn plant seedlings, so the total number of corn plant seedlings was 75 seedlings that were sown for 1 month in the greenhouse, and then inoculated with mycorrhizal solution after the seedlings were 2 weeks old. The parameters observed in this study were mycorrhizal fungal growth (days), mycorrhizal fungal development (cm2), and the percentage of mycorrhizal fungal infection in corn plant roots (%). Based on the results of the study, the type of solution media treatment had a significant effect on the growth and development of endomycorrhiza from the roots of meranti stands. The effective solution media in this study was in the A4 treatment, namely a mixture of rice washing water waste and brown sugar which produced the best average growth and development of endomycorrhiza. In addition, the effectiveness of endomycorrhizal fungi is able to spur the growth of corn plants used as applied media. Endomycorrhizal fungi propagated using this solution procedure have the potential to be developed as a biofertilizer and can improve soil fertility.
KERAGAMAN FENOTIPE DAUN MERANTI PUTIH (Anthoshorea montigena) DI PULAU SERAM Christofer Wattimena; Johan M. Matinahoru; Irwanto Irwanto
MARSEGU : Jurnal Sains dan Teknologi Vol. 2 No. 11 (2026): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/2.11.2026.826-841

Abstract

This study examines the phenotypic diversity of White Meranti (Anthoshorea montigena) leaves in Seram Island by analyzing leaf morphology across four sites: Honitetu, Latu, Haya, and Masihulan. The research aims to identify similarities in leaf characteristics among locations and to evaluate the influence of environmental factors on phenotypic variation. A purposive sampling survey was conducted, with both quantitative and qualitative data analyzed using ANOVA and regression. Results revealed significant variation in leaf area, primary vein length, and number of secondary veins. Environmental factors contributed differently at each site: light intensity affected 32.54% of leaf area in Honitetu, air humidity 30.86% in Latu, soil fertility 51.98% in Haya, and humidity 22.41% in Masihulan. The phenogram indicated 85% similarity between Honitetu and Latu, 80% with Haya, and greater divergence with Masihulan. These findings provide valuable insights for future breeding and conservation of White Meranti (Anthoshorea montigena).
Co-Authors Andjela Sahupala Christofer Wattimena Elva Dasilvia Rianekuay Fransina S. Latumahina Irwanto Irwanto Miranda H. Hadijah Muhammad Gofal Yahya Tuharea Nuel Patrik Radjabaycolle Bunga