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All Journal Medical Laboratory Journal The Journal General Health and Pharmaceutical Sciences Research
Devina Adira Azzahra
Universitas Duta Bangsa Surakarta
Health Professions Nursing Public Health Other

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Analisis Tren Penggunaan Teknologi Loop-Mediated Isothermal Amplification (LAMP) sebagai Alternatif PCR: Kajian Literatur Erika Dwi Susanti; Elsa Trisna Sari; Devina Adira Azzahra; David Valentino Iskandar; Nasywarana Putri Panjaitan; Khotimah Nur Ramadhani; Juwita Ramadhani Octavianingrum; Elsa Oktavia Ramadhani; Liss Dyah Dewi Arini
The Journal General Health and Pharmaceutical Sciences Research Vol. 4 No. 1 (2026): The Journal General Health and Pharmaceutical Sciences Research
Publisher : LPPM STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/tjghpsr.v4i1.1044

Abstract

Gene amplification technology plays a crucial role in medical diagnostics. While Polymerase Chain Reaction (PCR) is widely regarded as the standard method for amplifying nucleic acids, its reliance on a thermocycler restricts its use in settings with limited resources. As an alternative, Loop-Mediated Isothermal Amplification (LAMP) has gained attention for its ability to perform specific and fast amplification at a constant temperature, eliminating the need for temperature cycling. The goal of this literature review is to examine the global developments and usage of LAMP technology, comparing it with PCR as a possible alternative. To achieve this, a systematic review of scientific articles from both international and national sources was conducted, focusing on the application, validation, and comparison of LAMP with PCR. The findings show a rising number of publications and patents related to LAMP, especially in areas like diagnosing infectious diseases and conducting point-of-care (POC) testing. This growth is attributed to the benefits of LAMP, such as its high sensitivity, quick results, and ease of detection through visual means without the need for electrophoresis. In summary, LAMP is showing great promise as a transformative diagnostic tool. The rapid growth in its adoption suggests that it is becoming a strong alternative to PCR for real-time and affordable diagnostic applications in various settings.
Pemanfaatan Mikroskop dalam Menanggapi Fenomena Resistensi Antibiotik pada Bakteri Patogen Desi Rahmasari; Devina Adira Azzahra; Hanayu Anindya Nareswari; Nasywarana Putri Panjaitan; Rizka Felithia; Elsa Oktavia Ramadhani; Liss Dyah Dewi Arini
Calory Journal Medical Laboratory Journal Vol. 3 No. 3 (2025): September : Medical Laboratory Journal
Publisher : LPPM STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/caloryjournal.v3i2.778

Abstract

Antibiotic resistance has emerged as one of the most critical challenges in modern healthcare. Pathogenic bacteria that were once susceptible to antibiotics have developed survival mechanisms, making infections increasingly difficult to treat. Understanding and combating this issue requires advanced tools, with microscopy playing a pivotal role. Microscopes enable researchers to observe bacterial morphological changes, biofilm formation, and antibiotic-bacteria interactions at molecular levels. Various microscopy techniques – including light microscopy, electron microscopy, and fluorescence microscopy – have significantly contributed to both research and clinical diagnostics. Through a literature review approach, this article examines how microscopy facilitates antibiotic resistance studies and supports the development of more effective treatment strategies.
Co-Authors David Valentino Iskandar Desi Rahmasari Elsa Oktavia Ramadhani Elsa Trisna Sari Erika Dwi Susanti Hanayu Anindya Nareswari Juwita Ramadhani Octavianingrum Khotimah Nur Ramadhani Nasywarana Putri Panjaitan Rizka Felithia