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Fatimah Fatimah
Lambung Mangkurat University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Languange, Linguistic, Communication & Media

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Aktivitas Antibakteri Sabun Organik Berbasis Ekstrak Kopi Robusta (Coffea canephora) Terhadap Escherichia coli ATCC 8739 Fatimah Fatimah; Maya Istyadji; Yudha Irhasyuarna Fatimah
Jurnal Ilmiah BioSmart (JIBS) Vol. 12 No. 1 (2026): Jurnal Ilmiah BioSmart Volume 12 No. 1 2026
Publisher : Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jibs.v12i1.6592

Abstract

The purpose of this study was to determine the effectiveness of robusta coffee extract-based organic soap (Coffea canephora) against the growth of Escherichia coli ATCC 8739 bacteria. This study used a completely randomized design (CRD) with seven treatment groups of robusta coffee soap at concentrations of 10%, 25%, 50%, 75%, 100%, 150%, and 300%, a positive control (ampicillin), and a negative control (aquadest). The disc diffusion method was used to evaluate antibacterial activity. Data were analyzed using one-way ANOVA and Tukey's post-hoc test. The results showed that the inhibition zone diameter increased with increasing concentration of robusta coffee extract against E. coli. At concentrations ≥150%, the inhibition zone was equivalent to or greater than the positive control ampicillin, with significant differences (p < 0.05). These results suggest that robusta coffee soap has potential as an environmentally friendly natural antibacterial alternative and can promote the use of local robusta coffee.
Co-Authors Maya Istyadji Yudha Irhasyuarna Fatimah