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All Journal Arfak Chem: Chemistry Education Journal
Dina Alva Prastiwi
Sekolah Tinggi Analis Kimia Cilegon, Banten
Humanities Chemistry Education Social Sciences

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Comparison of the Antioxidant Activity of n-Hexane Extract of Gandaria Stem Bark Using DPPH and ABTS Assays Agus Malik Ibrahim; Dina Alva Prastiwi; Boima Situmeang
Arfak Chem: Chemistry Education Journal Vol. 9 No. 1 (2026): Arfak Chem
Publisher : Universitas Papua, Manokwari, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/accej.v9i1.1268

Abstract

This study aimed to evaluate and compare the antioxidant activity of the n-hexane extract of gandaria (Bouea macrophylla) stem bark using DPPH and ABTS methods. This research was conducted as a laboratory-based quantitative study. The extraction was performed using the maceration method with n-hexane as a solvent, yielding 6.2% extract. Antioxidant activity was determined from IC₅₀ values obtained by linear regression of concentration versus percentage inhibition. The results showed that the n-hexane extract exhibited strong antioxidant activity, with IC₅₀ values of 26.695 ± 0.892 ppm for the DPPH method and 17.28 ± 0.352 ppm for the ABTS method. The lower IC₅₀ value obtained in the ABTS assay indicates a higher radical-scavenging capacity than in the DPPH assay. These differences are likely due to variations in radical types and reaction mechanisms between the two assays. In conclusion, the n-hexane extract of Gandaria stem bark demonstrates strong antioxidant potential. The ABTS method showed greater sensitivity than the DPPH method in evaluating antioxidant activity. These findings suggest that Gandaria stem bark has potential as a natural antioxidant source, and further research is required to elucidate its active constituents.
Co-Authors Agus Malik Ibrahim Boima Situmeang