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All Journal Quagga: Jurnal Pendidikan dan Biologi
Rizka Kusuma Ningrum
Universitas Maarif Hasyim Latif
Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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Isolation of Hydrolytic Bacteria from Black Glutinous Rice Wash Water Indra Adi Wira Prasetya; Elsa Mega Suryani; Rizka Kusuma Ningrum
Quagga: Jurnal Pendidikan dan Biologi Vol 18 No 2 (2026): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v18i2.522

Abstract

Natural sources for hydrolytic enzymes produced by bacteria in demand in industry were increased. Hydrolytic bacteria can be found in agricultural and household waste, like black glutinous wash water. This study aims to identify and evaluate the presence and hydrolytic potential of bacteria in washing waste from fermented black glutinous rice. Fermented black glutinous rice washing water was used as the sample for bacterial isolation. The obtained isolates were screened for hydrolytic activity using modified Bushnell–Haas Agar supplemented with specific substrates to assess cellulolytic, amylolytic, and pectinolytic activities. Hydrolytic activity was expressed as a hydrolytic index to determine the most potent isolate, which was subsequently identified at the molecular level. A total of eight bacterial isolates were successfully obtained, among which one isolate, designated NKH4, showed the highest hydrolytic potential based on screening results. Molecular identification revealed that NKH4 is closely related to Bacillus tequilensis, with a similarity value of 97.96%. This species has previously been reported to produce various hydrolytic enzymes. The selected isolate NKH4 exhibited a qualitative hydrolytic index of 0.89, demonstrating its ability to hydrolyze starch, cellulose, and pectin. Therefore, NKH4, identified as Bacillus tequilensis, holds strong potential as a producer of hydrolytic enzymes and may serve as a promising candidate for further biotechnological applications.
Optimization of Antibacterial Production and Molecular Identification of Endophytic Bacteria Isolated from Papaya (Carica papaya L.) Seeds Esti Rizkiana Pratiwi; Nadiah Al Batati; Rizka Kusuma Ningrum
Quagga: Jurnal Pendidikan dan Biologi Vol 18 No 2 (2026): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v18i2.525

Abstract

Papaya seed endophytic bacteria have been reported to exhibit antibacterial activity against pathogenic microorganisms, including Staphylococcus aureus and Escherichia coli. However, their antibacterial production remains suboptimal. This study aimed to determine the optimal conditions for antibacterial production and to identify the most potent bacterial isolate through 16S rRNA gene-based molecular analysis. Three endophytic bacterial isolates (CPA2, CPA8, and CPA9) were evaluated. Characterization was performed based on pH variations (5, 7, and 9) and growth media (Nutrient Broth (NB) and Tryptic Soy Broth (TSB)), followed by antibiotic sensitivity testing against ciprofloxacin, amoxicillin, gentamicin, and tetracycline. Antibacterial activity was assessed using the Kirby–Bauer method against S. aureus and E. coli. The results showed that all isolates grew optimally in TSB medium at neutral pH (7). Ciprofloxacin and tetracycline exhibited stronger inhibitory effects compared to gentamicin and amoxicillin. Among the isolates, CPA2 in TSB media pH 7 demonstrated the highest antibacterial activity, producing inhibition zones of 30.2 ± 1.77 mm against S. aureus (very strong) and 11.8 ± 0.02 mm against E. coli (strong). Molecular identification revealed that isolate CPA2 was closely related to Bacillus cereus strain JCM 2152 with 99.90% similarity. In conclusion, papaya seed endophytic bacteria, particularly isolate CPA2, show significant potential as a source of antibacterial compounds under optimized culture conditions. These results have the potential as an alternative source of antibacterial agents
Co-Authors Elsa Mega Suryani Esti Rizkiana Pratiwi Indra Adi Wira Prasetya Nadiah Al Batati