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All Journal Jurnal SainHealth Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Quagga: Jurnal Pendidikan dan Biologi
Esti Rizkiana Pratiwi
Universitas Maarif Hasyim Latif
Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Other

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Uji Potensi Antibakteri Ekstrak Kulit Jeruk Nipis Citrus aurantifolia (Christm) Swingle Terhadap Vibrio sp. Indra Adi Wira Prasetya; Aziz Aziz; Evy Ratnasari Ekawati; Muhammad Sungging Pradana; Nadiah Al Batati; Esti Rizkiana Pratiwi
Jurnal SainHealth Vol 7, No 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.6731.9-14

Abstract

Lime (Citrus aurantifolia) is one of the herbal plants that is rich in benefits, especially as a herbal medicines. Besides of lime fruit, lime peel also has antibacterial properties such as essential oils which contain flavonoids which can inhibit bacterial growth. This research utilizes lime peel which will be extracted as a test material. The purpose of this research is to determine the antibacterial potential of lime peel extract against Vibrio sp. at various concentrations. This research used the well-diffusion method using MHA as a test medium to determine whether lime peel extract can inhibit the growth of Vibrio sp. Various concentrations and penicillin as a positive control used in this research to determine which concentration has a greatest potential for inhibiting the growth of Vibrio sp. Result of this study used qualitative analysis by comparing the clear zone size produced on the medium. The obtained result shown that 20%, 40%, 60%, 80% and 100% concentration of lime peel can forming a clear zone which mean they can inhibit the growth of  Vibrio sp. The best concentration used to inhibit Vibrio sp. is 100% concentration which produce 23.7 mm clear zone on MHA medium. Lime peel extract can be used to inhibit Vibrio sp. growth based on the research result.
Perbandingan Metode Membran Filter dan Metode Tabung Ganda Terhadap Kandungan Escherichia coli pada Air Bersih Lathifa Azkhiyati; Dheasy Herawati; Setyo Dwi Santoso; Esti Rizkiana Pratiwi; Elsa Mega Suryani
Jurnal SainHealth Vol 7, No 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.6732.15-21

Abstract

Escherichia coli bacteria are bacteria used as indicators of contamination. Bacteriological water testing can use a Membrane Filtration (MF) and a Multiple Tube-Fermentation (MTF). This study aims to compare the membrane filtration and Multiple Tube-Fermentation methods on the presence of Escherichia coli in clean water. The two methods are compared to determine the percentage difference in the results of the two tests. The research methodology uses a description of observation. 20 samples of water boreholes were used and tested for the presence of Escherichia coli. Calculation of bacterial groups uses SNI standard references regulated by the Minister of Health of the Republic of Indonesia. The results showed that the average number of  Escherichia coli bacteria by the Multiple Tube-Fermentation method was less than the Membrane Filtration method. There is a difference in the percentage of the results of the two tests by 40%. Multiple Tube Fermentation is more sensitive than the Membrane Filtration method for detecting the presence of Escherichia coli in clean water.Keywords : Water, Escherichia coli, Membrane filtration, Multiple tube-fermentation
PERBANDINGAN HASIL MIKROSKOPIS LANJUTAN PENDERITA TBC PARU SETELAH PENGOBATAN DENGAN RIWAYAT DM DAN TANPA DM DI PUSKESMAS KEDUNDUNG Esti Rizkiana Pratiwi; Frida Luthfia Mari; Halik Wijaya; Titik Sundari; Indra Adi Wira Prasetya
Jurnal SainHealth Vol 7, No 2 (2023): September 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i2.14355.68-72

Abstract

Comorbid diabetes mellitus (DM) in pulmonary tuberculosis (TB) patients may increase the risk of death even with or during TB treatment. Immunocompromised conditions due to DM increase the risk of infection and sputum conversion failure. The risk of recurrence in comorbid patient is also higher after treatment. Objective of this research is to describe the comparison of further microscopic examination of pulmonary tuberculosis patients without DM and pulmonary tuberculosis patients with a history of diabetes at the Kedundung Public Health Center. The study was conducted by observing each of 15 TB positive sputum samples accompanied by a history of DM and those without any history of DM 2 months post-treatment on further microscopic examination. Further microscopic examination showed that 100% of sputum samples of TB patients without a history of DM and receiving treatment gave negative results, while sputum samples of TB patients with DM history and receiving treatment, while sputum samples of patients who had received treatment showed results of 2+ by 80% and 3+ by 20% and there were no negative results for TB. These results prove that there is an effect of a history of DM disease on TB treatment outcomes. Keywords : Further microscopic examination, Pulmonary tuberculosis (TB), Diabetes Mellitus (DM)
Perbandingan Metode Membran Filter dan Metode Tabung Ganda Terhadap Kandungan Escherichia coli pada Air Bersih Lathifa Azkhiyati; Dheasy Herawati; Setyo Dwi Santoso; Esti Rizkiana Pratiwi; Elsa Mega Suryani
Jurnal SainHealth Vol. 7 No. 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.6732.15-21

Abstract

Escherichia coli bacteria are bacteria used as indicators of contamination. Bacteriological water testing can use a Membrane Filtration (MF) and a Multiple Tube-Fermentation (MTF). This study aims to compare the membrane filtration and Multiple Tube-Fermentation methods on the presence of Escherichia coli in clean water. The two methods are compared to determine the percentage difference in the results of the two tests. The research methodology uses a description of observation. 20 samples of water boreholes were used and tested for the presence of Escherichia coli. Calculation of bacterial groups uses SNI standard references regulated by the Minister of Health of the Republic of Indonesia. The results showed that the average number of  Escherichia coli bacteria by the Multiple Tube-Fermentation method was less than the Membrane Filtration method. There is a difference in the percentage of the results of the two tests by 40%. Multiple Tube Fermentation is more sensitive than the Membrane Filtration method for detecting the presence of Escherichia coli in clean water.Keywords : Water, Escherichia coli, Membrane filtration, Multiple tube-fermentation
PERBANDINGAN HASIL MIKROSKOPIS LANJUTAN PENDERITA TBC PARU SETELAH PENGOBATAN DENGAN RIWAYAT DM DAN TANPA DM DI PUSKESMAS KEDUNDUNG Esti Rizkiana Pratiwi; Frida Luthfia Mari; Halik Wijaya; Titik Sundari; Indra Adi Wira Prasetya
Jurnal SainHealth Vol. 7 No. 2 (2023): September 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i2.14355.68-72

Abstract

Comorbid diabetes mellitus (DM) in pulmonary tuberculosis (TB) patients may increase the risk of death even with or during TB treatment. Immunocompromised conditions due to DM increase the risk of infection and sputum conversion failure. The risk of recurrence in comorbid patient is also higher after treatment. Objective of this research is to describe the comparison of further microscopic examination of pulmonary tuberculosis patients without DM and pulmonary tuberculosis patients with a history of diabetes at the Kedundung Public Health Center. The study was conducted by observing each of 15 TB positive sputum samples accompanied by a history of DM and those without any history of DM 2 months post-treatment on further microscopic examination. Further microscopic examination showed that 100% of sputum samples of TB patients without a history of DM and receiving treatment gave negative results, while sputum samples of TB patients with DM history and receiving treatment, while sputum samples of patients who had received treatment showed results of 2+ by 80% and 3+ by 20% and there were no negative results for TB. These results prove that there is an effect of a history of DM disease on TB treatment outcomes. Keywords : Further microscopic examination, Pulmonary tuberculosis (TB), Diabetes Mellitus (DM)
Optimization of Antibacterial Production and Molecular Identification of Endophytic Bacteria Isolated from Papaya (Carica papaya L.) Seeds Esti Rizkiana Pratiwi; Nadiah Al Batati; Rizka Kusuma Ningrum
Quagga: Jurnal Pendidikan dan Biologi Vol 18 No 2 (2026): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v18i2.525

Abstract

Papaya seed endophytic bacteria have been reported to exhibit antibacterial activity against pathogenic microorganisms, including Staphylococcus aureus and Escherichia coli. However, their antibacterial production remains suboptimal. This study aimed to determine the optimal conditions for antibacterial production and to identify the most potent bacterial isolate through 16S rRNA gene-based molecular analysis. Three endophytic bacterial isolates (CPA2, CPA8, and CPA9) were evaluated. Characterization was performed based on pH variations (5, 7, and 9) and growth media (Nutrient Broth (NB) and Tryptic Soy Broth (TSB)), followed by antibiotic sensitivity testing against ciprofloxacin, amoxicillin, gentamicin, and tetracycline. Antibacterial activity was assessed using the Kirby–Bauer method against S. aureus and E. coli. The results showed that all isolates grew optimally in TSB medium at neutral pH (7). Ciprofloxacin and tetracycline exhibited stronger inhibitory effects compared to gentamicin and amoxicillin. Among the isolates, CPA2 in TSB media pH 7 demonstrated the highest antibacterial activity, producing inhibition zones of 30.2 ± 1.77 mm against S. aureus (very strong) and 11.8 ± 0.02 mm against E. coli (strong). Molecular identification revealed that isolate CPA2 was closely related to Bacillus cereus strain JCM 2152 with 99.90% similarity. In conclusion, papaya seed endophytic bacteria, particularly isolate CPA2, show significant potential as a source of antibacterial compounds under optimized culture conditions. These results have the potential as an alternative source of antibacterial agents
Pengenalan Produk Bioteknologi Yoghurt sebagai Minuman Probiotik Bagi Siswa Sekolah Menengah Atas di Sidoarjo Esti Rizkiana Pratiwi; Elsa Mega Suryani; Nadiah Al Batati; Indra Adi Wira Prasetya; Annisa Sekar Tejamaya; Nuzzulul Nisa Firamadhani
Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Vol. 11 No. 01 (2024): January
Publisher : Lembaga Penelitian, Penerbitan dan Pengabdian Masyarakat (LP3M) UNSIQ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32699/ppkm.v11i01.6322

Abstract

Bakteri asam laktat (BAL) adalah bakteri yang digunakan dalam produk fermentasi. Keberadaan BAL bermanfaat bagi kesehatan. Tujuan pengabdian ini adalah untuk meningkatkan pengetahuan siswa-siswi sekolah menengah atas di Sidoarjo terkait produk bioteknologi yoghurt. Bentuk kegiatan pengabdian adalah sosialiasi melalui edukasi cara pembuatan yoghurt, bakteri yang digunakan dalam pembuatan yoghurt dan manfaat mengkonsumsi yoghurt untuk kesehatan. Hasil pengabdian yaitu, beberapa siswa-siswi yang belum mengkonsumsi yoghurt, telah mencoba sampel yoghurt. Siswa-siswi mengetahui cara pembuatan yoghurt secara sederhana, mengetahui bakteri untuk pembuatan yoghurt serta manfaat yoghurt untuk kesehatan terutama kesehatan saluran pencernaan. Melalui edukasi ini, diharapkan siswa-siswi bisa membuat yoghurt secara mandiri di rumah dan mulai mengkonsumsi yoghurt untuk kesehatan.
Hubungan Kadar Glukosa Darah, Albumin dan Kreatinin Pada Penyembuhan Luka Pasien Diabetes Melitus: Association Between Blood Glucose, Albumin, and Creatinine Levels and Wound Healing in Patients With Diabetes Mellitus Adinda Hartini Ningtyas; Yenny Puspa Rini; Novi Ersanto; Esti Rizkiana Pratiwi
Jurnal SainHealth Vol. 10 No. 2 (2026): September 2026
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v10i2.17469.131-138

Abstract

Diabetes Mellitus (DM) is a degenerative disease with a continuously increasing prevalence and a risk of chronic complications in the form of wounds or diabetic ulcers that are difficult to heal. Beyond blood glucose levels, nutritional status reflected by serum albumin and creatinine levels are also thought to influence tissue regeneration, since chronic hyperglycemia, hypoalbuminemia, and the accumulation of uremic toxins can inhibit angiogenesis, fibroblast proliferation, and local immune responses at the wound site. This study aimed to analyze the effect of blood glucose, serum albumin and creatinine levels on the rate of wound healing in patients with Diabetes Mellitus at Klinik Sahabat Sehat Lamongan. The study employed a quantitative analytical observational design with a cross-sectional approach, involving 44 respondents selected through purposive sampling, with laboratory data and wound observations analyzed using bivariate chi-square tests and multivariate analysis. The results showed that all Three variables were significantly associated with the rate of wound healing (p = 0.000), and simultaneously had a significant effect, with blood glucose level being the most dominant factor.
Co-Authors Adinda Hartini Ningtyas Annisa Sekar Tejamaya Aziz Aziz Dheasy Herawati Elsa Mega Suryani Elsa Mega Suryani Evy Ratnasari Ekawati Frida Luthfia Mari Halik Wijaya Indra Adi Wira Prasetya Lathifa Azkhiyati Muhammad Sungging Pradana Nadiah Al Batati Nadiah Al Batati Novi Ersanto Nuzzulul Nisa Firamadhani Rizka Kusuma Ningrum Setyo Dwi Santoso Titik Sundari Yenny Puspa Rini