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Chemical characterization of White Burley tobacco stems at varying elevations for bio-oil optimization using FTIR Puspitasari, Risky Anggraeni; Dewanti, Beauty Suestining Diyah; Setiyawan, Hendrix Yulis
Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Vol 9, No 2 (2026)
Publisher : Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.afssaae.2026.009.02.3

Abstract

Tobacco stems (Nicotiana tabacum), often discarded as waste, have significant potential as a source of secondary metabolites, including bio-oil. This study examines the effect of elevation on the chemical composition of White Burley tobacco stems from lowland, midland, and highland regions in Lumajang District using Fourier Transform Infrared Spectroscopy (FTIR). The FTIR results revealed distinct variations in functional groups: the lowland sample showed strong O–H, C–H aliphatic, C=O, and C–O peaks, indicating the presence of oxygenated compounds typical of bio-oil. The midland sample exhibited O–H, C–H aliphatic, and C–O–C bands, suggesting volatile organic and polar compounds. The highland sample revealed O–H, C–H, C=O, and C–O peaks, indicating both polar and non-polar compounds. Physicochemical analysis of aqueous extracts prepared from the same lowland, midland, and highland stem samples showed that the pH values were similar across samples (8.35–8.59), with the lowland sample having the lowest density (1.032 g/mL). These attributes suggest the lowland sample is most favorable for bio-oil production due to its closer pH to diesel oil and lower density. The findings indicate that elevation influences tobacco stem metabolites and physical properties, with lowland stems showing the greatest potential for high-quality bio-oil production.
Dynamics of nicotine accumulation in Madura tobacco (Nicotiana tabacum L.): Effects of plant age and leaf position Hidayati, Bhekti Ayu; Setiyawan, Hendrix Yulis; Pranowo, Dodyk; Dewanti, Beauty Suestining Diyah; Suprayogi, Suprayogi
Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Vol 9, No 2 (2026)
Publisher : Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.afssaae.2026.009.02.10

Abstract

Nicotine is the primary alkaloid in tobacco (Nicotiana tabacum L.), playing a significant role in determining tobacco quality. The Madura variety (Prancak 95) is highly adaptable to dryland conditions, making it suitable for studying the dynamics and rate of nicotine formation at different growth stages and leaf positions. This study aimed to measure nicotine content in Madura tobacco leaves based on plant age and leaf position and to determine its formation rate between growth phases. The experiment employed a randomized block design (RBD) with a factorial pattern, comprising plant age (i.e., 25, 40, 55, 70, and 85 days after transplanting/DAT) and leaf position (i.e., lower, middle, and upper) in three replications. Nicotine content was analyzed using the acidimetric titration method (SNI 01-7134-2006), and the formation rate was calculated as the difference in nicotine content between growth stages divided by the time interval. Results showed that nicotine content increased from 1.24% (25 DAT) to 2.72% (85 DAT). Average nicotine contents were 2.43% (upper), 1.86% (middle), and 1.64% (lower leaves). At 85 DAT, the upper leaves contained about 43% more nicotine than that of the lower leaves. The highest rate of nicotine formation occurred at 70–85 DAT.