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Erni Mohamad
Gorontalo State University

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Analysis of Microplastic Abundance in Fish Landed from the Waters of South Leato Sea Moh. R. Darise; Weny J. A. Musa; Hendri Iyabu; Wiwin R. Kunusa; Erni Mohamad
Jurnal Akademika Kimia Vol. 15 No. 2 (2026)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24775185.2026.v15.i2.pp111-120

Abstract

Microplastics are plastic particles smaller than 5 mm, formed by the degradation of plastic materials in aquatic environments and potentially contaminating marine biota. This study aimed to investigate the presence of microplastics in fish caught. It landed in the waters of the South Leato Sea and to identify their physical characteristics and constituent polymer types. The research samples consisted of four fish species, namely skipjack tuna (Katsuwonus pelamis), scad (Decapterus macrosoma), deho (Euthynnus affinis), and oci (Selaroides leptoleptis), with the analyzed parts being the gastrointestinal tract and muscle tissue. Microplastic analysis was conducted using the National Oceanic and Atmospheric Administration (NOAA) method, which included Wet Peroxide Oxidation (WPO) for the degradation of organic matter, density separation for particle separation based on density, microscopic identification to determine the morphology and abundance of microplastics, and Fourier Transform Infrared (FTIR) spectroscopy analysis to identify the constituent polymer types. The results showed that the identified microplastics were categorized into three morphological forms: fibers, fragments, and films. Based on polymer analysis, the detected microplastics were predominantly composed of polyamide (nylon). The highest abundance was observed in gastrointestinal tract samples from oci and scad fish, at 0.6 particles per individual. In contrast, the lowest abundance was observed in skipjack tuna, at 0.2 particles per individual. These findings indicate variations in microplastic contamination levels among the fish species examined. This study provides important implications for understanding the extent of microplastic pollution in edible fish from the South Leato Sea and may serve as a scientific basis for environmental quality monitoring and mitigation efforts to reduce the risks of microplastic contamination in the food chain and human health.
Antioxidant Activity of Hydrolysate Protein in Skipjack Fish (Katsuwonus pelamis) Using the ABTS Method Fou F. Muksin; Deasy N. Botutihe; Erni Mohamad; Hendri Iyabu; Najmah Najmah
Jurnal Akademika Kimia Vol. 15 No. 2 (2026)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24775185.2026.v15.i2.pp142-149

Abstract

Skipjack tuna (Katsuwonus pelamis) bones are a byproduct of the fishing industry that are rich in protein and have potential as a source of bioactive peptides. This study aimed to determine the antioxidant activity of protein hydrolysates from raw and cooked skipjack tuna bones using the ABTS method. Hydrolysis was performed using bromelain at concentrations of 4%, 6%, and 8% at 55 °C for 4 hours pH 7.0), followed by enzyme inactivation at 85 °C for 20 minutes. Antioxidant activity was measured by IC50 determination using a UV-Vis spectrometer at 734 nm. The results showed that the antioxidant activity of skipjack tuna bone samples with the highest total protein content was observed at an enzyme concentration of 8%, namely 35.55% (raw fish bones) and 36.24% (cooked fish bones). The highest degree of hydrolysis in the raw fish bone sample reached 82.15 % and in the cooked fish bone sample reached 86.81 % with 8 % enzyme. Qualitative amino acid tests (ninhydrin, Biuret, xanthoprotein, and Millon) yielded positive results, indicating the presence of aromatic amino acids (tyrosine, tryptophan, phenylalanine) that contribute to antioxidant activity. The lowest IC50 value was obtained in the raw fish bone sample at an 8 % concentration, namely 10.03 mg/ml, and in the cooked fish bone sample at 6.19 mg/ml at an 8 % enzyme concentration. The higher the enzyme concentration, the higher the antioxidant activity.