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Screening of Amylolytic Bacteria from the Intestines of Tilapia (Oreochromis niloticus) Cultivated in Rice Fish Farming in Panembangan Village, Cilongok District, Banyumas salsa nabila; Kasprijo; Rima Oktavia Kusuma; Sri Marnani; Muhammad Ikhwan Ihtifazhuddin
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/p5vczh22

Abstract

Amylolytic bacteria in the digestive tract of tilapia are bacteria that produceamylase enzymes, which function to break down carbohydrates, thereby aidingthe digestive process. The presence of amylolytic bacteria is influenced by feedand the environment. The purpose of this study was to determine the proportionand activity index of amylolytic bacteria in the intestines of tilapia. This studyused an observational method with purposive sampling. Amylolytic bacteriawere isolated from the intestines of tilapia in Rice fish farming Blocks A and E.The successfully isolated bacteria were grown on 1% starch agar medium withan incubation time of 48 hours and observed at 24-hour intervals. Then, severalcharacteristic tests were carried out, including Gram staining, KOH, catalase,and oxidase tests. Based on the results of the study, the proportion of amylolyticbacteria was found in 10 isolates with a proportion of 44% and 12 isolates witha proportion of 48% in the intestines of tilapia from the two blocks. The averageamylolytic activity index obtained from block A was 0.96 ± 0.237, and fromblock E was 1.49 ± 0.501. The largest amylolytic activity index was obtainedin isolate KSA 19 with a value of 1.33 from block A, while from block E, thelargest amylolytic activity index value was 2.6 in isolate KSE 5.
FARMING OF NILE TILAPIA (Oreochromis niloticus) AT THE KRIDO YUWONO FARM IN PANEMBANGAN Emyl Rohayati; Kasprijo
Journal Of Artha Biological Engineering Vol. 4 No. 1 (2026)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/6r5e8b07

Abstract

Tilapia farming is the practice of raising fish from fry to marketable size to meet increasing consumer demand. This study aims to evaluate tilapia farming activities at the Krido Yuwono Panembangan Fish Farming unit. The research methods employed were direct observation and active participation in all farming processes to obtain comprehensive and accurate data. The variables observed included farming stages, fish length and weight, survival rate (SR), specific growth rate (SGR), and feed conversion ratio (FCR). The farming activities consisted of several stages, including pond preparation (drying, liming, fertilization, and water filling), seed stocking, feeding management, and water quality control. The results showed that the survival rate reached 86.67%, indicating a relatively high level of fish survival during the culture period. The specific growth rate was recorded at 1.77% per day, while the feed conversion ratio was 1.35, reflecting efficient feed utilization. Overall, the applied farming practices were effective and supported optimal tilapia production.