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All Journal Berkala Perikanan Terubuk BIOTROPIA - The Southeast Asian Journal of Tropical Biology Omni-Akuatika Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Akuakultur Sungai dan Danau Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Pengabdian Magister Pendidikan IPA Lempuk: Jurnal Ilmu Kelautan dan Perikanan Journal of Artha Biological Engineering
Sri Marnani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Veterinary Other

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Effect of Land Agricultural Fertilizer on Growth of Marine Single Cell Protein, Spirulina platensis, Chlorella vulgaris and Nannochloropsis Purnama Sukardi; Ferisa Wahyunika; Tjahyo Winanto; Norman Arie Prayogo; Taufan Harisam; Arif Mahdiana; Sri Marnani
Journal Omni-Akuatika Vol 15, No 2 (2019): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.424 KB) | DOI: 10.20884/1.oa.2019.15.2.764

Abstract

This study aimed to determine the growth rate and protein content in Nannochloropsis oculata, Chlorella vulgaris, Spirulina platensis were cultured using agricultural fertilizers. The agricultural fertilizers used were Urea, ZA and TSP. Each single cell protein was cultured using the three types of fertilizers with a ratio of Urea: ZA and TSP as follows (A) 1: 2: 1 [(10:20:10 g / L)], (B) 2: 2: 1 [(20:20:10 g / L)] and (C) 3: 2: 1 [(30:20:10 g / L)], respectively. The results showed that the best ratio of Urea, ZA and TSP fertilizers to growth of Spirulina, Nannochloropsis oculata and Chlorella vulgaris was C [30:20:10 (g / L)] treatment. However, phyto-protein content in Nannochlorophsis differed significantly between fertilization treatments, C fertilization yielded the highest protein content (28.75±0.05%), when compared with A (25.13±0.01%) and B (25.14±0.02%), respectively. In Chlorella vulgaris, all fertilization treatments showed very significant differences, B fertilization (28.24%±00.006) yielded the highest phyto-protein content, if compared to A (23.63% ± 0.003) and C (19.74% ±0.006 ), respectively. All fertilization treatments showed very significant differences (P <0.05) on Spirulina platensis. The highest content of phyto-protein (62.68 + 0.05%) was present in treatment C, when compared to A (52.18 + 0.05%) and B (62.37 + 0.01%) treatments, respectively.
POLA PENYERAPAN KUNING TELUR DAN PERKEMBANGAN ORGANOGENESIS PADA STADIA AWAL LARVA IKAN SENGGARINGAN (Mystus nigriceps) Taufik Budhi Pramono; Sri Marnani
Berkala Perikanan Terubuk Vol 37, No 1 (2009): Februari 2009
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (696.198 KB) | DOI: 10.31258/terubuk.37.1.%p

Abstract

The observation was conducted to study the yolk absorption activity andmorphological development on the initial state of senggaringan (catfish) fishlarvae. Larvae used for this research were from the artificial spawning ofbroodstock, reared in fiber tank. The result showed that newly hatched larvae has3.02 mm ±0.14 total length and 1.1202 mm3 yolk volume. During yolk absorptionperiod, organogenesis development and length added also occurred. Larvaedevelopment occurred after hacthing involved the eye spot (23 hours, 1 minute),mouth opening (25 hours, 21 minutes) and pigmentation (50 hours, 5 minutes),.Organogenesis development and the total length increment improve, following theincrease of yolk absorption rate.
Imotilisasi, Kadar Glukosa dan Sintasan Ikan Nila (Oreochromis sp) Dalam Larutan Infusum Daun Durian (Durio zibethinus) Taufik Budhi Pramono; Anandita Ekasanti; Sri Marnani; R Panji Sulaeman
Jurnal Akuakultur Sungai dan Danau Vol 7, No 2 (2022): Oktober
Publisher : Universitas Batangahari Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/akuakultur.v7i2.78

Abstract

Durian leaves have been used extensively to aid in the anesthetic process in various types of fish. The purpose of this study was to determine the immotilization capacity and stress response and survival of Tilapia under anesthesia. The RAL study design (4x4) was carried out with the durian leaf infusion treatment at concentrations of 2%, 4%, 6% and 8%. The fastest induction and sedation times were at 8% and 6% concentrations, which were 12.33 minutes and 13.35 minutes, respectively. The highest survival rate was at a concentration of 2% which reached 100%. Blood glucose levels increased due to anesthesia, ranging from 67.75 to 134.25 mg/dL
Tepung Daun Lamtoro (Leucaena leucochepala) Pada Pakan Terhadap Performa Pertumbuhan Ikan Nilem (Osteochilus hasselti) Marnani, Sri; Pramono, Taufik Budhi; Rakhma, Yulia Nur

Publisher : Fishery Product Technology Study Program, Yudharta University, Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/lempuk.v2i1.4169

Abstract

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Ramuan jamu herbal pada pakan terhadap performa pertumbuhan ikan nila (Oreochromis sp) Pramono, Taufik Budhi; Marnani, Sri; Junaidi, Teuku; Setyawan, Agung Cahyo; Nasikhah, Ais Lulu; Wulan, Triana Sri

Publisher : Fishery Product Technology Study Program, Yudharta University, Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/lempuk.v2i2.4520

Abstract

Immunity and growth were problems in main focus in fish farming bussiness. The use of herbal ingredients in feed can be a solution. The aim of this research was to determine the effect of adding herbal supplements to feed in increasing the growth of Tilapia. The method used was a field experiment bya selecting tilapia in pond (8x3 m2). Adding natural supplements to feed begins with making herbal concoctions, then supplementing them with 50 mL/Kg feed..Fish were given feed at 3%/body weight for 1 month. The absolute growth in lenght and weight were 7.09±1.38 cm and 136.05±27.48 g. The feed conversation ratio was around 1.28, the feed efficiency was around 77.7% and the survival rate reached 100%. Herbal concoctions can increase immunity and optimal growth. .
EFFECT OF ASTAXANTHIN ON THE COLOR INTENSITY OF GUPPY FISH (Poecilia reticulata) soedibya, petrus; Hawari , Abidzar; Kasprijo, Kasprijo; Marnani, Sri; Muslih, Muslih; Fitriadi, Ren
Journal Of Artha Biological Engineering Vol. 2 No. 1 (2024)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/42gtk346

Abstract

Guppy fish is one type of ornamental fish which is in great demand due to its interesting color variations. Color of ornamental fish will be brighter if the feed contains color pigments. The study aims to determine the effect of Astaxanthin supplement in pellets on the color brightness of guppies and to find the best dose of Astaxanthin supplement in pellets on the color brightness of guppies. The method used in the study was complete randomized design (CRD) consisting of five treatments (Astaxanthin supplements of 0%, 2%, 3%, 4% and 5%) and three replications. The experimental fish were male guppy fish Strain HB Red ± 3 cm length with a density of 3 fish/aquarium. Feeding was done twice a day and siphoning was done every afternoon. The parameters observed were Chroma value, survival rate and water quality. The results show that the supplement of Astaxanthin in pellets successfully increase the brightness of guppies and the best dose of Astaxanthin supplement in pellets was 5% with an increase in Chroma value of 4.82%. The survival rate obtained was 100%. Water quality includes an average temperature of 26-27 ℃ and pH of 6-7 and dissolved oxygen (DO) was 6.95-7.93 ppm
EFFECT BRIGHTNESS COLOR GUPPY FISH (Poecilia reticulata) soedibya, petrus; Gultom, Noris; Marnani, Sri; Muslih, Muslih; Fitriadi, Ren
Journal Of Artha Biological Engineering Vol. 2 No. 2 (2024)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/jypcxk46

Abstract

Guppy fish (Poecilia reticulata) is a popular ornamental fish because of their unique color patterns, the color of ornamental fish will fade if their feed does not contain enough pigmentation to keep them bright. This study aimed to determine the effect of adding Spirulina sp. powder with different dosage to the feed on the color brightness of guppy fish. This study used a completely randomized design (CRD) with five treatments (feed added with Spirulina sp. powder as much as 0%, 0.3%, 0.6%, 0.9%, and 1.2%) and three replications. The test fish were male HB Red guppy fish with a size of 3 cm and a density of 3 fish/aquarium. Water changes (siphoning) was done every morning, and feeding was done twice a day. The parameters observed in this study were the chroma value, survival rate, and water quality. The result showed that the effect of adding Spirulina sp. powder to the feed at a dose of 0.9% and 1.2% had a significant effect on the color brightness. The survival rate indicates that the outcomes were not significantly different between treatments from a 100% success rate. The adding of Spirulina sp. powder increases the brightness of Guppy fish. The brightness color is very important in the Guppy fish farming, because color is the main indicator in ornamental fish. The water has a good quality, with an average temperature of 25.8-26°C and a pH of 7.9-8.0.
GROWTH PERFORMANCE AND INTESTINAL Aeromonas spp. OF BONYLIP BARB (Osteochilus vittatus) AFTER RECEIVING DIET CONTAINING SALT AND HERBAL CONCOCTION SUPPLEMENTATION Syakuri, Hamdan; Soedibya, Petrus Hary Tjahja; Marnani, Sri; Ekasanti, Anandita; Kasprijo, Kasprijo; Kusuma, Rima Oktavia; Wijaya, Rudy; Nugrayani, Dewi; Listiowati, Emyliana; Palupi, Mustika
Indonesian Aquaculture Journal Vol 19, No 2 (2024): (December, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.2.2024.111-122

Abstract

Salt and herbal supplementations in fish feed are expected to promote aquaculture productivity, including for slow-growing fish such as Bonylip barb (Osteochilus vittatus). The objective of this study was to evaluate the growth performance and occurrence of Aeromonas spp. in the intestine of Bonylip barb fed a diet supplemented with salt and herbs. This experiment was conducted using a completely randomized design with four treatments: 1) control, 2) salt 3% supplementation, 3) herbal supplementation, and 4) Salt 3% and herbal supplementation. Growth performance evaluation included weight gain, relative growth rate, and specific growth rate after 30 and 60 days of rearing.  Bacterial samples were isolated on trypticase soy agar (TSA) and glutamate starch phenol red agar (GSP) media. Yellow colonies on GSP were counted and confirmed as Aeromonas spp. via molecular identification based on 16S rDNA sequence. Results showed that herbal supplementation, either alone or in combination with 3% salt, insignificantly increased the fish growth performance in the short-term (30 days), but tended to decrease it over the long-term (60 days). Salt supplementation consistently decreased fish growth performance but improved fish survival rates. Aeromonas isolates comprised more than 60 % of the culturable intestinal bacteria in all treatments. Furthermore, herbal supplementation appeared to increase the percentage of Aeromonas spp. in the intestine, with identified species including Aeromonas veronii, Aeromonas caviae, and Aeromonas jandaei.
ISOLASI, IDENTIFIKASI, DAN ANALISIS EKSPRESI GEN PENGKODE GROWTH HORMONE PADA IKAN SIDAT (Anguilla bicolor) Syakuri, Hamdan; Wibowo, Satrio Haryu; Ekasanti, Anandita; Soedibya, Petrus Hary Tjahja; Marnani, Sri; Listiowati, Emyliana; Nugrayani, Dewi; Sukardi, Purnama
Jurnal Riset Akuakultur Vol 19, No 1 (2024): (Maret 2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.1.2024.69-83

Abstract

Gen hormon pertumbuhan (growth hormone, GH) perlu dipelajari untuk mendukung domestikasi ikan sidat Anguilla bicolor di Indonesia. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi gen pengkode GH serta menganalisis ekspresinya pada sampel ikan sidat A. bicolor. Primer untuk amplifikasi gen GH ikan sidat A. bicolor didesain berdasarkan sekuen gen GH dari beberapa spesies ikan sidat lain yang tersedia di GenBank. Sampel cDNA hipofisa dan otak ikan sidat digunakan untuk amplifikasi gen GH. Hasil amplifikasi disekuensing dan hasilnya dianalisis menggunakan analisis BLAST, multiple sequences alignment, hormon signature, dan filogenetik. Analisis ekspresi gen GH dilakukan menggunakan teknik realtime PCR dengan metode delta-delta CT pada 14 sampel ikan sidat (23,1 ± 19,6 g; 24,5 ± 4,2 cm). Fragmen DNA sepanjang 486 bp berhasil diamplifikasi dan disekuensing. Sekuen gen GH A. bicolor memiliki similaritas nukleotida sebesar 98,49-99,14% jika dibandingkan dengan gen GH ikan sidat A. anguilla, A. australis, dan A. japonica. Sekuen parsial tersebut secara in silico diketahui mengkode bagian dari protein GH sepanjang 155 asam amino (aa). Sekuen asam amino protein GH A. bicolor sangat mirip dengan sekuen spesies ikan sidat lain dengan hanya tiga perbedaan asam amino dan membentuk satu percabangan pada pohon filogenetik. Tingkat ekspresi gen GH pada sampel ikan sidat memiliki variasi yang tinggi. Hasil penelitian ini dapat menjadi dasar untuk studi selanjutnya khususnya berkaitan dengan peran gen GH dalam pertumbuhan ikan sidat A. bicolor.The growth hormone (GH) gene needs to be studied to support the domestication of the Anguilla bicolor eel in Indonesia. This study aimed to isolate and identify the GH coding gene and analyze its expression in samples of the eel A. bicolor. Primers for amplification of the GH gene of A. bicolor eels were designed based on GH gene sequences from several other eel species available in GenBank. Pituitary and brain cDNA samples of eel were used for GH gene amplification. The amplification results were sequenced and the results were analyzed using BLAST analysis, multiple sequence alignment, hormone signature, and phylogenetic analysis. The GH gene expression analysis was carried out using the real-time PCR technique with the delta-delta CT method on 14 eel samples (23.1 ± 19.6 g; 24.5 ± 4.2 cm). The 486 bp DNA fragment was successfully amplified and sequenced. The GH gene sequence of A. bicolor has a nucleotide similarity of 98.49-99.14% when compared with the GH gene of other eel species A. anguilla, A. australis, and A. japonica. This partial sequence was found in silico to code 155 amino acids (aa) GH protein. The amino acid sequence of the A. bicolor GH protein is very similar to that of other eel species with only three amino acid differences and forms one branch on the phylogenetic tree. The expression level of the GH gene in eel samples had high variations. The results of this study could be a basis for further studies, especially regarding the role of the GH gene in the growth of the eel A. bicolor. 
Tepung Daun Lamtoro (Leucaena leucochepala) Pada Pakan Terhadap Performa Pertumbuhan Ikan Nilem (Osteochilus hasselti) Marnani, Sri; Pramono, Taufik Budhi; Rakhma, Yulia Nur
Lempuk: Jurnal Ilmu Kelautan dan Perikanan Vol. 2 No. 1 (2023): Juni
Publisher : Fishery Product Technology Study Program, Yudharta University, Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/lempuk.v2i1.4169

Abstract

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Co-Authors Agung Sudaryono Arif Mahdiana Ekasanti, Anandita Emyliana Listiowati, Emyliana Ferisa Wahyunika Fitriadi, Ren Frentina Murti Sujadi Gultom, Noris Harisam, Taufan Hawari , Abidzar Islamy, R Adharyan Junaidi, Teuku Kasprijo, Kasprijo Kusnendar, Rinaldi Kusuma, Baruna Kusuma, Rima Oktavia Lilik Setiyaningsih Mardika, Hanisya Putri Kania Muslih Muslih Nasikhah, Ais Lulu Norman Arie Prayogo Norman Arie Prayogo, Norman Arie Nugrayani, Dewi Nurhabib, Asro Palupi, Mustika Purnama Sukardi Purnama Sukardi Putra, joni Johanda R Panji Sulaeman Rakhma, Yulia Nur Saprudin Setyawan, Agung Cahyo soedibya, petrus Soedibya, Petrus Hary Tjahja Syakuri, Hamdan Taufan Harisam Taufik Budhi Pramono Taufik Budhi Pramono Taufik Budhi Pramono Tjahyo Winanto Tohap Simangunsong Wibowo, Satrio Haryu Wijaya, Rudy Winanto, Tjahyo Wulan, Triana Sri Yansah, Noprie Yohanes Harvinda