This study aims to determine the effect of ecoprint batik activities on the fine motor skills of 5-6-year-old children at Pertiwi Pemwilda Kindergarten. The study used a quantitative approach with a quasi-experimental design and a Non-Equivalent Control Group Design. The study sample consisted of 20 children selected using a purposive sampling technique, consisting of 10 children in the experimental group who were given ecoprint batik activities and 10 children in the control group who were given collage activities. Data collection techniques included observation, testing, and documentation. Data were analyzed using descriptive statistics and the non-parametric Wilcoxon Signed Rank Test with the help of SPSS version 26. The results of the descriptive analysis showed that the average fine motor skills of the experimental group increased from 16.00 in the pre-test to 20.70 in the post-test, while the control group increased from 15.80 to 16.60. The results of the Wilcoxon test showed that the experimental group obtained a Z value = -2.859 with a significance of 0.004 (< 0.05), while the control group obtained a significance value of 0.066 (>0.05), so that the increase in the control group was not statistically significant. The effect size value of r = 0.904 indicates that ecoprint batik activities have a large influence on improving children's fine motor skills, especially in aspects of eye and hand coordination, finger flexibility, movement accuracy, and independence in completing tasks. Thus, ecoprint batik activities have a significant effect on improving the fine motor skills of children aged 5-6 years at Pertiwi Pemwilda Kindergarten.