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All Journal Agrivet: Jurnal Ilmu-ilmu Pertanian dan Peternakan
Syafiradewi Yulianti
Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry

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Kajian biosintesis dan akumulasi beta-karoten pada tanaman makanan ternak serta implikasinya terhadap kualitas nutrisi hijauan Syafiradewi Yulianti; Mansyur; Iin Susilawati
Agrivet : Jurnal Ilmu-Ilmu Pertanian dan Peternakan (Journal of Agricultural Sciences and Veteriner) Vol. 14 No. 1 (2026): Juni
Publisher : Fakultas Pertanian Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/agrivet.v14i1.18959

Abstract

Beta-carotene is the primary provitamin A carotenoid and natural antioxidant in forage plants, yet its biosynthesis and accumulation in tropical forages remain insufficiently characterised. This review systematically examined the biosynthetic pathways, regulatory factors, and nutritional implications of beta-carotene in forage plants using 45 peer-reviewed articles selected through a PRISMA-adapted protocol. Beta-carotene biosynthesis proceeds via the plastidial MEP pathway, with phytoene synthase (PSY) and lycopene beta-cyclase (LCYB) as principal regulatory enzymes. Accumulation varies substantially across forage species and is governed by harvest age, light intensity, temperature, and post-harvest management, with silage conserving up to 75–85% of initial beta-carotene content compared with losses of 70–90% during hay-making. In ruminants, an estimated 20–70% of ingested beta-carotene survives ruminal degradation (Wyss, 2020; Nozière et al., 2021; Calderón et al., 2020) and is subsequently absorbed and converted to vitamin A, supporting reproductive performance, immune function, and milk quality. Tropical legumes such as Leucaena leucocephala and Stylosanthes guianensis are rich beta-carotene sources, whereas data for Indonesian-specific cultivars of Napier grass, gamal (Gliricidia sepium), and indigofera (Indigofera zollingeriana) remain scarce. Strategic forage selection, optimised harvest intervals, and targeted supplementation are recommended to maximise beta-carotene delivery to ruminants under tropical conditions.
Analisis konsentrasi dan kemurnian Deoxyribo Nucleic Acid (DNA) dari rumput pakan ternak Syafiradewi Yulianti; Romi Zamhir Islami; Mansyur; Estria Furry Pramudyawardani
Agrivet : Jurnal Ilmu-Ilmu Pertanian dan Peternakan (Journal of Agricultural Sciences and Veteriner) Vol. 12 No. 2 (2024): Desember
Publisher : Fakultas Pertanian Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/agrivet.v12i2.10618

Abstract

This research was conducted at the Biotechnology Research and Testing Lab of the Faculty of Animal Husbandry, Padjadjaran University, and the Rice Standard Instrument Testing Center (BBPSI Padi), Subang Regency, West Java starting on September 20 to December 22, 2023. The purpose of this study was to evaluate the effectiveness of the hipure Kit in isolating grass Deoxyribo Nucleic Acid (DNA) by ensuring adequate concentration and cleanliness of samples from contaminants for subsequent molecular applications. The ideal DNA concentration for PCR is 20-50 ng/µL, with an A260/A280 absorbance ratio of about 1.8-2.0 and an A260/A230 ratio above 2.0, indicating the absence of contamination from proteins, salts, carbohydrates, or other organic compounds. The methodology of this study was a descriptive exploration involving descriptive statistical analysis to evaluate the variation in DNA concentration between samples, using spreadsheet software to facilitate data management. The results of the Nanodrop spectrophotometric analysis showed that there variations in DNA concentration between samples, but generally within a range adequate for laboratory applications such as PCR and sequencing. In addition, the HiPure Plant DNA Kit proved effective in producing high-concentration DNA with clear and defined DNA bands in electrophoresis and effectively minimizing contaminants. Further optimization of the extraction protocol, such as adjusting the incubation time or buffer concentration, is recommended to improve DNA concentration and purity. This step will ensure higher DNA quality for downstream applications such as PCR.
Co-Authors Estria Furry Pramudyawardani Iin Susilawati Mansyur Romi Zamhir Islami