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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Pembelajaran dan Biologi Nukleus
Mandayu Anestesia Hayyu Palupi
Department of Agrotechnology, Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 60294, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media

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Characteristics and Insecticidal Potential of Ecoenzymes Derived from Vegetable Waste and Various Sugars Dita Megasari; Wiwin Windriyanti; Mandayu Anestesia Hayyu Palupi; Anisah Qurrotu Aini; Nolla Dwi Elvira; Syaiful Khoiri
Jurnal Ilmu Pertanian Indonesia Vol. 31 No. 1 (2026): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.31.1.121

Abstract

Vegetable waste is the second largest category of household organic waste, following grains. Its underutilization at the household level is an environmental concern, but it also has potential as a raw material for ecoenzyme synthesis. Ecoenzymes created by fermentation may be used as environmentally friendly alternatives to chemical pesticides. However, only a few research have investigated the effect of various sugar types on the insecticidal efficiency of ecoenzymes generated from vegetable waste. The goal of this study was to create ecoenzymes from vegetable waste and assess their insecticidal properties. Ecoenzymes were prepared using a 3:1:10 ratio of vegetable waste, sugar, and water. The vegetable waste comprised of cabbage, lemongrass, and red onion peels, while the sugars were molasses, brown sugar, and granulated sugar, resulting in nine different compositions. Fermentation lasted three months. Each 10% ecoenzyme solution was tested for insecticidal activity against Crocidolomia pavonana larvae in their second instar stage. All ecoenzyme formulations exhibited physical characteristics associated with successful fermentation, such as an acidic pH (3.1−3.6), dark brown coloration, and a strong sour fragrance. Bioassays revealed that ecoenzymes had insecticidal effects through larval mortality and feeding inhibition. The mixture with lemongrass and molasses had the highest mortality (66.67%) and strongest antifeedant effect. Variations in waste type and sugar supply have a substantial impact on ecoenzyme performance. The findings demonstrate that vegetable waste may be effectively transformed into ecoenzymes with insecticidal characteristics, indicating their potential for application in sustainable pest management. Keywords: Crocidolomia pavonana, organic waste, plant-based pesticide, sustainable agriculture
Screening and Identification of Fusarium sp. Associated with Wilt Disease in Shallot Plants (Allium cepa L. var. aggregatum) Mandayu Anestesia Hayyu Palupi; Sri Wiyatiningsih; Arika Purnawati
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 3: Jurnal Pembelajaran Dan Biologi Nukleus September 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i3.9597

Abstract

Background: Allium cepa L. var. Aggregatum is an important horticultural commodity in Indonesia, particularly in East Java. However, shallot production is affected by Fusarium wilt, locally known as moler disease. Accurate identification of the associated Fusarium is important for supporting appropriate disease management. This study aimed to screen and characterize Fusarium sp. associated with wilt symptoms in shallot plants from Probolinggo and Nganjuk, East Java, through isolation, morphological characterization, and PCR amplification of the internal transcribed spacer (ITS) region. Methodology: Disease incidence was assessed based on symptomatic plants in five raised beds at each location. Three fungal isolates, P1 from Probolinggo and NG2 and NG3 from Nganjuk, were characterized morphologically and subjected to PCR using ITS1 and ITS4 primers. Findings: Disease incidence was 10.88% in Probolinggo and 0.45% in Nganjuk, with Probolinggo showing an approximately 24-fold higher incidence. All three isolates showed white to white-cream cottony colonies, hyaline and septate hyphae, and oval to ellipsoidal microconidia, consistent with Fusarium. PCR amplification produced a single band of approximately 750 bp in all isolates. Contribution: These findings provide baseline information on Fusarium associated with shallot wilt in Probolinggo and Nganjuk and support further sequencing-based identification.
Co-Authors Anisah Qurrotu Aini Arika Purnawati Dita Megasari Nolla Dwi Elvira Sri Wiyatiningsih Syaiful Khoiri Wiwin Windriyanti