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All Journal Jurnal Pembelajaran dan Biologi Nukleus
Sri Wiyatiningsih
Departement of Agrotechnology, Faculty of Agriculture, Pembangunan Nasional “Veteran” Jawa Timur University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media

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Screening and Identification of Fusarium sp. Associated with Wilt Disease in Shallot Plants (Allium cepa L. var. aggregatum) Mandayu Anestesia Hayyu Palupi; Sri Wiyatiningsih; Arika Purnawati
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 3: Jurnal Pembelajaran Dan Biologi Nukleus September 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i3.9597

Abstract

Background: Allium cepa L. var. Aggregatum is an important horticultural commodity in Indonesia, particularly in East Java. However, shallot production is affected by Fusarium wilt, locally known as moler disease. Accurate identification of the associated Fusarium is important for supporting appropriate disease management. This study aimed to screen and characterize Fusarium sp. associated with wilt symptoms in shallot plants from Probolinggo and Nganjuk, East Java, through isolation, morphological characterization, and PCR amplification of the internal transcribed spacer (ITS) region. Methodology: Disease incidence was assessed based on symptomatic plants in five raised beds at each location. Three fungal isolates, P1 from Probolinggo and NG2 and NG3 from Nganjuk, were characterized morphologically and subjected to PCR using ITS1 and ITS4 primers. Findings: Disease incidence was 10.88% in Probolinggo and 0.45% in Nganjuk, with Probolinggo showing an approximately 24-fold higher incidence. All three isolates showed white to white-cream cottony colonies, hyaline and septate hyphae, and oval to ellipsoidal microconidia, consistent with Fusarium. PCR amplification produced a single band of approximately 750 bp in all isolates. Contribution: These findings provide baseline information on Fusarium associated with shallot wilt in Probolinggo and Nganjuk and support further sequencing-based identification.
Co-Authors Arika Purnawati Mandayu Anestesia Hayyu Palupi