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All Journal Journal of Microbiology, Biotechnology and Conservation
Latifah Ningrum Abdillah
Politeknik Kesehatan YRSU Dr. Rusdi Medan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology

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Identifikasi Molekuler Bakteri Simbion Spons Spongia sp. Penghasil Antibakteri Latifah Ningrum Abdillah; Sri Muri Dasa Wardhani
Journal of Microbiology, Biotechnology and Conservation Vol. 2 No. 1 (2026): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/3vtdhq40

Abstract

This study aims to identify antibacterial-producing symbiotic bacteria in sponges using a molecular approach based on 16S ribosomal RNA (16S rRNA) genes as an initial step towards the sustainable use of marine biological resources. A total of six symbiotic bacterial isolates were successfully isolated from Spongia sp. sponges and exhibited differences in colony morphology characteristics. All isolates were tested for antibacterial activity against Escherichia coli and Bacillus subtilis. The test results showed that all isolates had the ability to inhibit the growth of test bacteria with inhibition zone diameters ranging from 5.0–8.5 mm against E. coli and 6.0–9.0 mm against B. subtilis. Among all isolates, Sp2 exhibited the highest antibacterial activity and was broad-spectrum, with stronger inhibitory power against Gram-positive bacteria. Isolate Sp2 was further molecularly identified using the 16S rRNA gene. Amplification results showed a DNA fragment of approximately 520 base pairs. Phylogenetic analysis based on the 16S rRNA gene sequence placed isolate Sp2 within the Bacillus genus, with the closest affiliation to Bacillus subtilis and supported by high bootstrap values, indicating a strong genetic relationship. Overall, the results of this study indicate that isolate Sp2 is the most promising symbiotic bacterium of the sponge Spongia sp. as a producer of antibacterial compounds against E. coli and B. subtilis. This isolate has the potential to be further developed as a source of antibacterial bioactive compounds, thus requiring further research to identify and characterize the active compounds it produces.
Identifikasi Bakteri Coli fecal dan Coli non fecal pada Minuman Es Teh Jumbo yang Dijual di Jalan Karya, Kecamatan Medan Barat Sri Muri Dasa Wardhani; Latifah Ningrum Abdillah; Muhammad Rifqi
Journal of Microbiology, Biotechnology and Conservation Vol. 2 No. 2 (2026): Journal of Microbiology, Biotechnology and Conservation (jMBC)
Publisher : Master’s Program in Biology, Faculty of Mathematics and Natural Sciences, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/sctebj95

Abstract

Iced tea is a popular beverage among the public, but the processing and serving of this drink carry a risk of bacterial contamination. This study aimed to identify the presence of fecal and non-fecal coliform bacteria in jumbo-sized iced tea sold on Jalan Karya, Medan Barat District. The study employed a quantitative descriptive method using the Most Probable Number (MPN) technique on eight samples. Testing was conducted in a preliminary stage using Lactose Broth (LB) and a confirmation stage using Brilliant Green Lactose Bile Broth (BGLB), with incubation at 44.5°C for fecal coliforms and 37°C for non-fecal coliforms. The results showed that all samples tested positive for both fecal and non-fecal coliform bacteria. The MPN values for fecal coliforms ranged from 10 to ≤979 MPN/100 mL, while those for non-fecal coliforms ranged from 265 to ≤979 MPN/100 mL. All results exceeded the maximum limits set forth in Regulation of the Minister of Health of the Republic of Indonesia No. 492/MENKES/PER/IV/2010. Thus, none of the jumbo iced tea samples met the microbiological quality requirements.
Co-Authors Muhammad Rifqi Sri Muri Dasa Wardhani