Identifikasi Bakteri Coli fecal dan Coli non fecal pada Minuman Es Teh Jumbo yang Dijual di Jalan Karya, Kecamatan Medan Barat

Sri Muri Dasa Wardhani (Politeknik Kesehatan YRSU Dr. Rusdi Medan)
Latifah Ningrum Abdillah (Politeknik Kesehatan YRSU Dr. Rusdi Medan)
Muhammad Rifqi (Politeknik Kesehatan YRSU Dr. Rusdi Medan)

Journal of Microbiology, Biotechnology and Conservation
Vol. 2 No. 2 (2026): Journal of Microbiology, Biotechnology and Conservation (jMBC)

Iced tea is a popular beverage among the public, but the processing and serving of this drink carry a risk of bacterial contamination. This study aimed to identify the presence of fecal and non-fecal coliform bacteria in jumbo-sized iced tea sold on Jalan Karya, Medan Barat District. The study employed a quantitative descriptive method using the Most Probable Number (MPN) technique on eight samples. Testing was conducted in a preliminary stage using Lactose Broth (LB) and a confirmation stage using Brilliant Green Lactose Bile Broth (BGLB), with incubation at 44.5°C for fecal coliforms and 37°C for non-fecal coliforms. The results showed that all samples tested positive for both fecal and non-fecal coliform bacteria. The MPN values for fecal coliforms ranged from 10 to ≤979 MPN/100 mL, while those for non-fecal coliforms ranged from 265 to ≤979 MPN/100 mL. All results exceeded the maximum limits set forth in Regulation of the Minister of Health of the Republic of Indonesia No. 492/MENKES/PER/IV/2010. Thus, none of the jumbo iced tea samples met the microbiological quality requirements.