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All Journal Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Lentera Negeri
Lariman Lariman
Mulawarman University
Agriculture, Biological Sciences & Forestry Humanities Education Environmental Science Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Social Sciences Other

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Callus-derived silver nanoparticles from myrmecodia tuberosa: green synthesis and antioxidant properties Yanti Puspita Sari; Sandrila Sandrila; Enos Tangke Arung; Linda Oktavianingsih; Samsurianto Samsurianto; Lariman Lariman; Sus Trimurti
Lentera Negeri Vol. 7 No. 1 (2026): Lentera Negeri
Publisher : Indonesian Institute For Counseling, Education and Therapy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29210/992170

Abstract

Green synthesis of Silver nanoparticles (AgNPs) using plant-based materials is an environmentally friendly path for the synthesis of silver nanoparticles instead of traditional chemical methods. This study presents the first biosynthesis of AgNPs using an ethanolic extract obtained from in vitro callus cultures of Myrmecodia tuberosa Jack, an endangered medicinal epiphyte and conservation‑friendly biotechnological platform. Cotyledon explants were successfully induced high-frequency friable, green callus and the ethanolic extract acted as reducing and stabilizing agent. UV‑Vis spectroscopy revealed surface plasmon resonance peaks at 420–450 nm with a visible color change from light brown to dark brown, confirming the AgNP formation. The most intense and well-defined SPR peak indicating optimal nanoparticle synthesis was observed after 6–8 days static incubation at 35°C using 2 mM AgNO₃. In the DPPH radical scavenging assay, it could moderate antioxidant activities, with IC₅₀ = 51.80±0.035 μg mL⁻¹) which was more than that of parent callus extract (IC₅₀ = 58.91±0.097μgmL⁻¹). This improvement is probably a result of synergistic effects between silver core and phytochemical capping layer. These results indicate that callus cultures of M. tuberosa is an environmentally friendly, sustainable and conservation-friendly source of reducing metabolites for the preparation of green nanomaterials applicable in biomedical practices.
Phytochemical characterization and antihyperglycemic activity of kemumu (Colocasia gigantea Hook. f.) leaf stalk juice in glucose-loaded mice (Mus musculus L.) Reni Kurniati; Nur Azizah Muhti’ah; Retno Aryani; Rudy Agung Nugroho; Ratna Kusuma; Lariman Lariman; Annisa Nurul Ilmi
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 9, No 1 (2026): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v9i1.4938

Abstract

Kemumu leaf stalk juice (Colocasia gigantea Hook. f.) is traditionally used to control blood sugar levels. However, scientific evidence is still limited and further studies are required for this purpose. This study aims to analyze the phytochemical content and test the ability of kemumu leaf stalk juice to reduce blood sugar levels in glucose-loaded mice. Secondary metabolites examined included tannins, alkaloids, phenolics, saponins, steroids, triterpenoids, and flavonoids. The antihyperglycemic activity was evaluated using 20 adult male mice (Mus musculus L.) as an animal model. First, fasting blood sugar levels were measured, then the mice were given 50% glucose. After 30 minutes, blood sugar levels were measured again. The mice were divided into 5 groups: 2 control groups and 3 treatment groups. The negative control group mice received 0.2 ml of distilled water, and the positive control group mice received glibenclamide at 0.65 mg/kg BW. Mice in treatment groups 1, 2, and 3 were given 0.05 ml/20 g BW, 0.1 ml/20 g BW, and 0.2 ml/20 g BW of kemumu juice, respectively. Blood sugar levels were measured every 30 minutes for up to 180 minutes. Data were analyzed using ANOVA, and if significant differences were found, an LSD test was performed. The results showed that kemumu juice contains alkaloids, phenolics, and flavonoids, which have antidiabetic properties. Doses of 0.1–0.2 ml were as effective as glibenclamide in the positive control group.
Co-Authors Annisa Nurul Ilmi Enos Tangke Arung, Enos Tangke Linda Oktavianingsih Nur Azizah Muhti’ah Ratna Kusuma Reni Kurniati Retno Aryani Rudy Agung Nugroho Samsurianto Sandrila Sandrila Sus Trimurti Yanti Puspita Sari