Damiana Nofita Birhi
Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Flores, East Nusa Tenggara, Indonesia

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PRELIMINARY PHYTOCHEMICAL ANALYSIS OF THE METHANOL EXTRACT OF RHODODENDRON RENSCHIANUM FLOWERS, ENDEMIC TO KELIMUTU NATIONAL PARK, INDONESIA, USING UV–VIS SPECTROPHOTOMETRY Damiana Nofita Birhi; Faustina De Yesu Prisila Abi; Antonia Fransiska Laka; Ni Putu Vidya Primarista
Walisongo Journal of Chemistry Vol. 9 No. 1 (2026): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology UIN Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v9i1.32005

Abstract

Rhododendron renschianum is an endemic Indonesian species characterized by bell-shaped flowers with bright red petals and a yellowish base. This study aimed to document the presence of and quantify major phytochemical groups in the methanolic extract of R. renschianum flowers. Phytochemical screening yielded positive results for triterpenoids, tannins, flavonoids, alkaloids, and quinones. However, quantitative analysis using UV–Vis spectrophotometry indicated an apparent triterpenoid content of 2685.48 ± 123.49 mg UA/g, highlighting a limitation of UV–Vis analysis for investigating reactive compounds such as triterpenoids. The remaining compounds, in ascending order of content, were flavonoids, alkaloids, tannins, and quinones, with values of 5.98 ± 0.70 mg QE/g, 90.09 ± 67.09 mg CAF/g, 109.24 ± 1.44 mg TA/g, and 130.93 ± 30.19 mg HQ/g, respectively. These findings add to the baseline data for the initial investigation of the secondary metabolite content of this endemic species and provide a preliminary basis for further pharmacological research.