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Hamed Kioumarsi
Department of Animal Science Research, Agricultural Research, Education and Extension Organization (AREEO), Rasht-4915685915 (Iran)

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Investigating the Antioxidant, Antimicrobial and Sensory Effects of Honey Propolis Extract as a Natural Preservative in Beef Burgers Forough Fakhraei; Alireza Masoud Nia; Fatemeh Zia Ziabari; Hamed Kioumarsi
Bioactivities Vol. 3 No. 2 (2025): Bioactivities
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/bioactivities.2963-654X.335

Abstract

In this study, the antioxidant, antimicrobial and sensory effects of honey propolis extract as a natural preservative in beef burgers were investigated. First, propolis extract was produced at different percentages (0.5, 1.0, and 2.0%), and the control treatment, containing 0% propolis extract, was produced with a beef burger. The pH, antioxidant 2,2-diphenyl-1-picrylhydrazyl (DPPH), thiobarbituric acid (TBA), total volatile base nitrogen (TVB-N), and microbial characteristics (total microbial count, Escherichia coli, Staphylococcus aureus, mold, and yeast) and sensory characteristics (color, smell, texture and overall acceptance) during the storage period were subsequently investigated over a period of 8 days (0, 4, and 8). The results of the evaluation of different properties revealed that adding propolis extract to hamburger samples caused a significant decrease in pH, TBA, TVN, and microbial properties (total microbial count, E. coli, S. aureus, mold, and yeast) (p < 0.05). On the other hand, the amount of antioxidant property and sensory evaluation (color, smell, texture, and overall acceptance) also increased with the addition of propolis extract (p < 0.05). Additionally, the duration of storage caused a significant increase in pH, TBA, TVN, and microbial characteristics (total microbial count, E. coli, S. aureus, and mold and yeast), and a reduction in antioxidant properties and sensory evaluation (color, smell, texture, and overall acceptability) were found in hamburger samples (p < 0.05). The use of 2% propolis extract improved the oxidation properties and maintained the sensory properties during 8 days of storage for hamburger. Therefore, the use of 2% propolis extract improves the antioxidant and antimicrobial properties of hamburgers during storage and is a superior treatment.
A Comparative Study on Bioactivities of Aqueous Extracts from Various Parts of Moringa Oleifera Annissa Annissa; Hanif Amrulloh; Hamed Kioumarsi
Bioactivities Vol. 3 No. 2 (2025): Bioactivities
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/bioactivities.2963-654X.457

Abstract

Moringa oleifera (M. oleifera) is widely recognized as a rich source of natural antioxidants; however, systematic comparisons of the phytochemical composition and biological activities of aqueous extracts obtained from different plant organs remain limited. This study aimed to comparatively evaluate the phytochemical profiles, antioxidant capacity, antibacterial activity, and antifungal activity of aqueous extracts prepared from the leaves, twigs, stem bark, and wood of M. oleifera. Qualitative phytochemical screening, determination of total phenolic and total flavonoid contents, Fourier-transform infrared spectroscopy, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, and resazurin-based microdilution assays against representative bacterial and fungal pathogens were performed. Leaf and twig extracts exhibited the greatest phytochemical diversity, whereas the wood extract showed the simplest composition. The leaf extract contained the highest total phenolic content (683.95 ± 16.74 μg gallic acid equivalents mL⁻¹) and total flavonoid content (514.08 ± 26.12 μg catechin equivalents mL⁻¹), followed by the twig, bark, and wood extracts. Consistently, the leaf extract demonstrated the strongest antioxidant activity, with 64.60 ± 0.69% radical scavenging activity, approaching that of the reference antioxidant (71.72 ± 0.56%). Fourier-transform infrared spectroscopy confirmed the presence of hydroxyl, carbonyl, aromatic, and ether functional groups associated with phenolic and flavonoid compounds, particularly in leaf and twig extracts. Antibacterial and antifungal activities followed the same overall trend, with leaf extracts exhibiting the greatest inhibitory effects, followed by twig, bark, and wood extracts. These findings demonstrate that the distribution of bioactive phytochemicals is strongly organ-dependent and closely associated with antioxidant and antimicrobial activities. The study identifies M. oleifera leaves as the most promising source of water-extractable antioxidant compounds and supports their potential application in the development of sustainable functional foods, nutraceuticals, and phytopharmaceutical products.