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The comparison of the effectiveness of shoe flower (Hibiscus rosa-sinensis L.) and roselle flower (Hibiscus sabdariffa L.) infusions as alternative reagents for the examination of Soil Transmitted Helminths (STH) eggs Acivrida Mega Charisma; Eka Octaviana Rahayu; Farida Anwari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 1 (2024): Advancements in Biomedical Research: Insights from Medical Laboratory and Technol
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i1.5409

Abstract

Worm infections remain a significant health challenge globally, including in Indonesia, where they continue to contribute to a majority of health issues. Soil-transmitted helminths (STH), particularly intestinal nematode parasites, are predominant in causing these infections. Microscopic examination with 2% eosin staining is a conventional method for diagnosing worm infections. However, exploring alternative staining agents, such as anthocyanin-rich extracts from Hibiscus rosa-sinensis L. (hibiscus flower) and Hibiscus sabdariffa L. (roselle flower), presents a promising avenue. These plants possess anthocyanin compounds, resulting in a captivating red pigment and making them suitable for utilization as natural coloring agents. This study aimed to evaluate the efficacy of hibiscus and roselle infusions as alternative staining reagents for examining STH egg, compared to the standard 2% eosin staining method. Adopting a true experimental design, samples of STH worm egg suspensions were subjected to different concentrations of hibiscus and roselle infusions. The results revealed varying staining outcomes with different concentrations. However, a 1:1 concentration ratio of hibiscus and roselle infusions demonstrated effective staining against STH worm eggs. In conclusion, this research underscores the comparative effectiveness of hibiscus and roselle infusions as alternative staining reagents for STH egg examination, with 2% eosin serving as the control. These findings hold potential implications for improving diagnostic techniques in managing worm infections.
Formulation and Physical Stability Evaluation of Herbal Liniment Containing Various Ginger Varieties (Zingiber officinale) Aulia Fauziah Bening Hanani; Muhammad Arzam Fadhluddin Al Fikri; Melia Fitriana; Anugraheny Ayu Pramita; Rima Via; Farida Anwari; Herni Setyawati
Open Global Scientific Journal Vol. 5 No. 1 (2026): Open Global Scientific Journal
Publisher : Research and Social Study Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70110/ogsj.v5i1.93

Abstract

Background: Musculoskeletal pain is a common health problem that can reduce the quality of life. Topical liniments are often used to relieve pain; however, long-term use of synthetic liniments carries a risk of skin irritation. Herbal-based liniments made from ginger rhizome (Zingiber officinale) are relatively safer, provide a warming sensation, and among their biological activities are anti-inflammatory and analgesic effects.Aims: This research aims to evaluation of herbal liniment identify containing various ginger varieties.Methods: This experimental study aimed to evaluate the physical stability of a herbal liniment with variations of ginger: red, gajah, and emprit. Three formulas were created, each containing 10% ginger extract in virgin coconut oil as a base. The evaluation was conducted over 28 days at room temperature (25 ± 2 °C), covering organoleptic properties, pH, homogeneity, viscosity, and transferable volume.Results: The results showed good physical stability: pH 5.00–5.66, viscosity 5.00–5.60 cP, maintained homogeneity, and volume >95%. Statistical analysis (p > 0.05) revealed no significant differences among the formulas. However, the dark brown color did not meet this standard.Conclusion: This herbal liniment is stable and has the potential to be developed as a topical preparation based on natural ingredients; however, color optimization is needed for better consumer acceptance.