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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Sri Wilarso Budi
Department of Silviculture, Faculty of Forestry, Institut Pertanian Bogor, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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DNA BARCODE CHARACTERIZATION OF MISTLETOE INFESTATION IN TEAK CLONAL SEED ORCHARD (CSO) IN PADANGAN, EAST JAVA PROVINCE, INDONESIA Zainal Muttaqin; Sri Wilarso Budi; Basuki Wasis; Corryanti Corryanti; Iskandar Zulkarnaen Siregar
BIOTROPIA Vol. 24 No. 2 (2017): BIOTROPIA Vol. 24 No. 2 August 2017
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2755.603 KB) | DOI: 10.11598/btb.2017.24.2.651

Abstract

For effective teak plantation management, early detection system in controlling teak mistletoe requires various basic information, including degree of infestation and accuracy of the species names. Mistletoe infestations in teak and mistletoe species name have been reported, but there are still problems in identifying the correct species or subspecies due to morphological similarity. The objective of this study was to clarify the species identity of hemi-parasitic mistletoe plants, which were found in teak Clonal Seed Orchard (CSO) in Padangan, East Java Province, Indonesia using DNA barcodes. Species identification of teak mistletoe based on leaf morphological characteristics and universal DNA barcode regions (i.e. matK and rbcL) were carried out. The results showed that the Canonical Discriminant Analysis (CDA) could differentiate Dendrophthoe pentandra and Macrosolen tetragonus based on leaf morphological characteristics. Variables having high correlation to distinguish both species were length of petiole, width of the widest leaf, number of secondary leaf veins, leaf base shape, aspect ratio, form factor and perimeter ratio of diameter. The results of DNA barcoding showed that the two DNA barcode regions presented good amplification and sequence results. Both DNA barcode regions successfully differentiated two species i.e. D. pentandra and M. tetragonus which belong to Loranthaceae family and have similar leaf morphological characteristics. Those regions were also able to identify Viscum articulatum and other species belonging to Santalaceae family. These results suggested that the two DNA regions could become recommended universal DNA barcode for identifying teak mistletoe.
JABON (Anthocephalus cadamba Roxb.) POTENCY FOR REMEDIATING LEAD (Pb) TOXICITY UNDER NUTRIENT CULTURE CONDITION Luluk Setyaningsih; Yadi Setiadi; Sri Wilarso Budi; Hamim Hamim; Didy Sopandie
BIOTROPIA Vol. 25 No. 1 (2018): BIOTROPIA Vol. 25 No. 1 April 2018
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2018.25.1.712

Abstract

Information on metal adaptation in plants is essential to understand the mechanism of heavy metal remediation, including lead (Pb), mediated by forest plants in contaminated land. This study aimed to identify the adaptation mechanism of jabon (Anthocephalus cadamba Roxb.) seedlings to excessive lead levels based on the tolerance index of growth performance and lead transport to plant tissue. The seedlings were exposed to lead Pb(NO₃)₂​ at concentrations of 0, 0.5, 1, 1.5, 5, and 10 mM in nutrient culture for 15 days. The results showed that the tolerance index (TI) of the seedlings significantly decreased with Pb exposure up to 1.5 mM, although the TI values remained above 75%. All seedlings died at Pb concentrations of 5 mM and above. Lead accumulated in all parts of the seedlings, with the highest concentration found in the leaves (735.9 ppm) under 0.5 mM Pb exposure. Pb was transported to the upper portion of the seedlings, as indicated by a transport factor (TF) greater than 1. The results suggest that jabon can tolerate excessive Pb exposure up to 1.5 mM and has potential as a phytoremediator plant.
Co-Authors Basuki Wasis Corryanti Corryanti Didy Sopandie Hamim Hamim Iskandar Zulkarnaen Siregar Luluk Setyaningsih Yadi Setiadi Zainal Muttaqin