<![CDATA[Vitamin E dan selenium adalah dua nutrisi penting yang berperan dalam menjaga kesehatan tubuh serta mendukung fungsi vital organ. Peran vitamin E (VE) dan selenium (Se) sebagai antioksidan dapat dimanfaatkan untuk meningkatkan performa penetasan itik. Penelitian ini bertujuan untuk menganalisis struktur histologi bursa fabricius embrio itik setelah inklusi in-ovo vitamin E dan selenium. Penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri atas empat perlakuan, yaitu K0 (kontrol), K1 (inklusi in-ovo 0,1 mL NaCl 0,89%), K2 (inklusi in-ovo 0,1 mL VE dan Se), dan K3 (inklusi in-ovo 0,15 mL VE dan Se). Perlakuan diberikan pada saat telur tetas berumur 14 hari, inkubasi diakhiri pada hari ke-26. Pembuatan preparat bursa menggunakan metode paraffin dan pewarnaan hematoksilin eosin. Parameter yang diamati berupa jumlah dan diameter plika, jumlah dan diameter folikel, tebal epitelium, diameter lumen, tebal korteks dan medula. Data diuji dan dianalisis dengan Analysis of Varians (ANOVA) pada taraf signifikansi 5% (α = 0,05) dan Kruskal-Wallis. Hasil menunjukkan adanya perbedaan nyata (P<0,05) pada jumlah dan diameter folikel. Jumlah plika, diameter plika, tebal epitelium, diameter lumen, tebal korteks dan tebal medula menunjukkan hasil berbeda tidak nyata (P>0,05). Berdasarkan hasil penelitian disimpulkan bahwa injeksi vitamin E dan selenium secara in-ovo berpotensi mengoptimalkan perkembangan struktur histologi folikel bursa fabricius, pada gilirannya mendukung kerja sistem imun itik hibrida. Vitamin E and selenium are two important nutrients that play a role in maintaining body health and supporting vital organ function. The role of vitamin E (VE) and selenium (Se) as antioxidants can be utilized to improve duck hatchability. This study aims to analyze the histological structure of the bursa fabricius of duck embryos after in-ovo inclusion of vitamin E and selenium. This study used a completely randomized design (CRD) consisting of four treatments, namely K0 (control), K1 (in-ovo inclusion of 0.1 mL of 0.89% NaCl), K2 (in-ovo inclusion of 0.1 mL of VE and Se), and K3 (in-ovo inclusion of 0.15 mL of VE and Se). The treatments were administered when the eggs were 14 days old; egg incubation ended on day 26. Bursa preparations were made using the paraffin method and stained with hematoxylin-eosin. The parameters observed were the number and diameter of plicae, the number and diameter of follicles, the thickness of the epithelium, the diameter of the lumen, and the thickness of the cortex and medulla. The data were analyzed using Analysis of Variance (ANOVA) at a significance level of 5% (α = 0.05) and the Kruskal-Wallis test. The results showed a significant difference (P<0.05) in the number and diameter of follicles. The number and diameter of plicae, epithelium thickness, lumen diameter, cortex and medulla thickness showed no significant differences (P>0.05). Based on the results of this study, it can be concluded that in-ovo injection of VE and Se has the potential to optimize the histological structure of the bursa fabricius follicles, which in turn supports the immune system of hybrid ducks.]]>
