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All Journal Rengganis Jurnal Pengabdian Masyarakat Jurnal Kolaboratif Sains
Firman Fajar Perdhana
Program Studi Ilmu dan Teknologi Pangan, Universitas Mataram
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Sosialisasi CPPB-IRT untuk Meningkatkan Kesadaran Keamanan Pangan Produk Lokal Masyarakat Desa Bintang Rinjani Firman Fajar Perdhana; Made Gendis Putri Pertiwi; Baiq Haulia Ariyanti; Rendi Gunawan; Fitri Inayati; Alfu Laily; Rizki Muhammad Ibnu Sabilillah; Mulia Aridho; Rizal Fahmi; Nila Susila Wati; Mey Rizka R. Putri; Risna Lestari
Rengganis Jurnal Pengabdian Masyarakat Vol. 6 No. 1 (2026): Mei 2026
Publisher : Pendidikan Matematika, FKIP Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/rengganis.v6i1.1165

Abstract

Bintang Rinjani Village possesses abundant agricultural potential, yet food downstreaming is hindered by low literacy in food safety and business legality. This community service activity aims to socialize Good Manufacturing Practices for Home Industries and the licensing procedures for Food Production Certificate for Home Industry. The program was conducted in January 2024, involving 24 participants through interactive lectures and discussions using visual media. The effectiveness was measured using pre-test and post-test instruments with a 4-point Likert scale. Analysis results showed a significant increase in participants' understanding, with the average score rising by 21.35%, from 58.13% (moderate criteria) to 79.48% (high criteria). In conclusion, this program successfully enhanced community literacy toward food safety standards and legal aspects, serving as a crucial foundation for developing competitive and consumer-safe local home industries.
Rapid Detection of HDC Gene in Enterobacter aerogenes from Fish Products Using In Silico PCR for Food Safety and Allergy Risk Assessment: Deteksi Cepat Gen HDC pada Enterobacter aerogenes dari Produk Perikanan Menggunakan In Silico PCR untuk Penilaian Keamanan Pangan dan Risiko Alergi Firman Fajar Perdhana; Muhammad ‘Aidil Febriandito; Setyaning Pawestri; Made Gendis Putri Pertiwi; Mutia Devi Ariyana; Lalu Unsunnidhal; Ines Marisya Dwi Anggraini; Lulu Diani Zuhdia
Jurnal Kolaboratif Sains Vol. 8 No. 3: Maret 2025 - Jurnal Kolaboratif Sains (JKS)
Publisher : Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/jks.v8i3.7038

Abstract

Histamine poisoning is a significant food safety concern associated with fish and fishery products. Enterobacter aerogenes is one of the histamine-producing bacteria capable of converting L-histidine into histamine through the histidine decarboxylase (HDC) enzyme. The accumulation of histamine in fish and fishery products can lead to allergic reactions and foodborne illnesses, highlighting the need for rapid detection methods. This study aimed to design and optimize primers targeting the HDC gene in E. aerogenes and validate their specificity using in silico PCR. The HDC gene sequence was retrieved from the NCBI GenBank database, and primers were designed using Primer3Plus software. The in silico PCR analysis was performed to assess the specificity of the primers against the genome of E. aerogenes and other histamine-producing bacteria species. The optimized Primer Pair 1 successfully amplified a 230 bp target region, showing high specificity for E. aerogenes in product size and annealing position with no cross-reactivity to other histamine-producing bacteria species. These findings demonstrate the potential of in silico PCR as a rapid and cost-effective screening tool for detecting histamine-producing bacteria in fish and fishery products. However, further in vitro validation is required to confirm the applicability of these primers in real-world food safety and allergy risk assessments.
Co-Authors Alfu Laily Baiq Haulia Ariyanti Fitri Inayati Ines Marisya Dwi Anggraini Lalu Unsunnidhal Lulu Diani Zuhdia Made Gendis Putri Pertiwi Mey Rizka R. Putri Muhammad ‘Aidil Febriandito Mulia Aridho Mutia Devi Ariyana Nila Susila Wati Rendi Gunawan Risna Lestari Rizal Fahmi Rizki Muhammad Ibnu Sabilillah Setyaning Pawestri