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IN VITRO ANTIOXIDANT ACTIVITY TEST OF ETHANOL EXTRACT OF PINK CHRYSANTHEMUM LEAVES (CHRYSANTHEMUM MORIFOLIUM) USING DPPH AND FRAP METHODS Nathin Loria , Saragih; Billy Johnson Kepel; Fona Dwiana Hermina Budiarso; Fatimawali Fatimawali; Aaltje Ellen Manampiring
Jurnal Impresi Indonesia Vol. 4 No. 2 (2025): Jurnal Impresi Indonesia
Publisher : Riviera Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58344/jii.v4i2.6277

Abstract

Non-communicable diseases (NCDs) are a major global health concern, with degenerative diseases accounting for >80% of premature deaths. The combination of lifestyle and environmental factors increases the production of free radicals in the body, which, when uncontrolled, cause oxidative stress that significantly contributes to various diseases. Antioxidants play a key role in stopping free radical chain reactions. Although the body has endogenous antioxidants, additional exogenous antioxidants are also needed. One source of exogenous antioxidants is pink chrysanthemum leaves, commonly known as an ornamental plant, which shows potential as an antioxidant source. This study aims to analyze phytochemical content and antioxidant activity of pink chrysanthemum leaf ethanol extract using DPPH and FRAP methods, compared with ascorbic acid. This study used laboratory experimental methods. The qualitative color tests for phytochemical screening. Antioxidant activity tested via DPPH and FRAP methods using UV-Vis spectrophotometer at 517 and 739 nm wavelengths. Results : Phytochemical screening showed positive results for steroids, tannins, phenols, flavonoids, saponins, and alkaloids. DPPH method, analyzed using regression line equation Y = 0.3547x + 45.267, showed IC50 13.344, while the ascorbic acid's 3.030. FRAP method, analyzed using regression line equation Y = 0.773x – 27.6, showed IC50 100.388, while the ascorbic acid's 18.174. The ethanol extract of pink chrysanthemum leaves contains steroids, tannins, phenols, flavonoids, saponins, and alkaloids, and possesses antioxidant activity that falls into the very strong category using the DPPH method and the strong category using the FRAP method.
IN VITRO EVALUATION OF THE ANTI-INFLAMMATORY POTENTIAL OF ETHANOL EXTRACT FROM PINK CHRYSANTHEMUM (CHRYSANTHEMUM MORIFOLIUM) Gloria Prisca Susana Angelique Baya; Aaltje Ellen Manampiring; Fatimawali Fatimawali; Hermina Budiarso; Billy Johnson Kepel
Jurnal Impresi Indonesia Vol. 4 No. 2 (2025): Jurnal Impresi Indonesia
Publisher : Riviera Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58344/jii.v4i2.6305

Abstract

Inflammation is the body's normal response to injury. However, acute and chronic inflammatory reactions can lead to various diseases. Nonsteroidal anti-inflammatory drugs (NSAIDs) have been proven effective as anti-inflammatory agents, but excessive and long-term use of NSAIDs can cause various side effects. Pink chrysanthemum flowers (Chrysanthemum morifolium) are one of many medicinal plants used as traditional medicine due to their content of compounds such as flavonoids, triterpenoids, and steroids, which have anti-inflammatory properties and the potential to serve as alternatives to NSAIDs. To identify active compounds and analyze the anti-inflammatory activity of ethanol extract from pink chrysanthemum flowers (Chrysanthemum morifolium). This study is an experimental laboratory research. The extraction method used was Microwave-Assisted Extraction (MAE). The anti-inflammatory activity of the ethanol extract of pink chrysanthemum flowers was measured using the red blood cell membrane stabilization method. The tests were performed using a UV-Vis spectrophotometer at extract concentrations of 50 ppm, 100 ppm, 200 ppm, 400 ppm, and 800 ppm. Pink chrysanthemum flowers contain several phytochemical compounds, including alkaloids, flavonoids, tannins, triterpenoids/steroids, and phenolics. The anti-inflammatory activity measurement showed that higher extract concentrations resulted in lower absorbance values and increased stabilization percentages, indicating anti-inflammatory activity that protects red blood cell membranes from damage. The ethanol extract of pink chrysanthemum flowers contains several phytochemical compounds with potential anti-inflammatory activity. The extract at a concentration of 800 ppm demonstrated the highest membrane stabilization ability, with a stabilization rate of 59.459%.