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All Journal Jurnal Pembelajaran dan Biologi Nukleus Jurnal Insan Pengabdian Indonesia
Prima Novitasari
Universitas Negeri Semarang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice

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A Comparative Review of Chitinase Enzymes from Microbial and Mammalian Origins and Their Roles in Health Applications Prima Novitasari; Maria Ulfa Nur Hidayanti; Maria Estela Karolina; Amalia An Nidha
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.7554

Abstract

Background: Chitinase is an enzyme crucial in chitin biodegradation, breaking the 1,4-N-α-acetyl-D-glucosamine bonds of its monomers. It is produced by a wide range of organisms, from microorganisms such as bacteria and fungi to macroorganisms including humans. Chitinases have been widely studied for applications in biological control, environmental management, and medical fields, including immune modulation and infection therapy. However, there is still a research gap due to the limited number of systematic comparative studies examining the structural, genetic, and functional differences among chitinases from various biological sources. This limitation hinders optimal and targeted use of chitinases in enzyme-based technologies, particularly for medical applications. Therefore, comprehensive studies are needed not only to describe chitinases by their sources but also to evaluate their practical potential and innovative opportunities for human health. Methods: This study uses a narrative literature review approach. Articles were collected, screened, and systematically organized according to their scope, focusing on chitinase sources, mechanisms of action, and applications in healthcare. Results: The analysis shows fundamental differences in domain structure, gene expression, and catalytic mechanisms of chitinases from bacteria, fungi, and humans. Bacterial chitinases exhibit environmental stability and serve as infection biomarkers, fungal chitinases excel in immunotherapy and bioactive compound production, and human chitinases, though less explored, show potential in precision diagnostics and inflammatory therapy. Conclusions: This cross-domain comparison maps structural and functional characteristics and opens opportunities for integrating cross-species enzyme functions for innovative enzyme-based biopharmaceuticals and precision therapies addressing modern pathogens
Edukasi Berbasis Mikrobiologi untuk Pencegahan Infeksi Luka pada Siswa Sekolah Menengah Pertama (SMP) Islam Al Azhar 14 Semarang Maria Estela Karolina; Prima Novitasari; Syifa Nurisma Putri; Dyah Ayu Shinta Ratnasari; Adelia Hanung Puspaningtyas; Luqman Alwi; Lutfi Muzaqi; Achmadi Achmadi; Umiyati Umiyati; Farriza El Zahra Kusuma; Revan Aji Sulistio; Alayna Farah Falastin; Florensia Indah Setyowati
Jurnal Insan Pengabdian Indonesia Vol. 4 No. 3 (2026): September : Jurnal Insan Pengabdian Indonesia
Publisher : PT. ALHAFI BERKAH INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62007/jouipi.v4i3.982

Abstract

Minor wounds are common among junior high school students, especially during physical activities and sports. A preliminary survey at Al Azhar 14 Islamic Junior High School, Semarang, showed that most students had experienced minor wounds but had limited knowledge of proper wound hygiene and infection prevention. The “Siswa Cerdas Mikrobiologi” community service program aimed to improve students’ knowledge of basic microbiology and wound care through interactive and experiential learning, in support of SDGs 3, 4, and 6. The program consisted of a pretest, interactive education sessions, hands-on science activities using microscopy and agar culture, student-created educational media, and a posttest with reflection. A total of 91 seventh- and eighth-grade students, mostly aged 13–14 years, participated. Results showed improved knowledge of wound care, with students in the good category increasing from 59% at pretest to 98% at posttest. Basic microbiology knowledge also increased from 85% to 96%. These findings indicate that microbiology-based education combined with interactive and hands-on methods can strengthen students’ health literacy in preventing wound infections. Program sustainability requires educational equipment and a formal implementation agreement with the school.
Co-Authors Achmadi Achmadi Adelia Hanung Puspaningtyas Alayna Farah Falastin Amalia An Nidha Dyah Ayu Shinta Ratnasari Farriza El Zahra Kusuma Florensia Indah Setyowati Luqman Alwi Lutfi Muzaqi Maria Estela Karolina Maria Estela Karolina Maria Ulfa Nur Hidayanti Revan Aji Sulistio Syifa Nurisma Putri Umiyati Umiyati