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All Journal Sehat Rakyat: Jurnal Kesehatan Masyarakat
Widaninggar Rahma Putri
Universitas 'Aisyiyah Yogyakarta
Dentistry Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Perbandingan Hasil Pemeriksaan Tuberkulosis Metode GeneXpert dan Ziehl-Neelsen di Rumah Sakit X Yogyakarta Andini Bilindade; Wiwit Probowati; Widaninggar Rahma Putri
Sehat Rakyat: Jurnal Kesehatan Masyarakat Vol. 4 No. 3 (2025): Agustus 2025
Publisher : Yayasan Pendidikan Penelitian Pengabdian Algero

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54259/sehatrakyat.v4i3.5455

Abstract

Tuberculosis is an infectious disease caused by the bacterium Mycobacterium tuberculosis. The tuberculosis (TB) detection methods used are the GeneXpert method and the Ziehl-Neelsen method. GeneXpert identifies the rpoB hotspot gene bacteria in Mycobacterium tuberculosis by analyzing deoxyribonucleic acid (DNA), while the TB detection method with Ziehl-Neelsen staining is a microscopic examination used to detect acid-fast bacilli (AFB), namely Mycobacterium tuberculosis bacteria. The purpose of this study was to determine the sensitivity, specificity and positive predictive value of the nucleic acid amplification test (GeneXpert) using sputum samples in patients with suspected pulmonary tuberculosis and compare it with microscopic examination (Ziehl-Neelsen staining) of Acid-Fast Bacilli (AFB). The method used is simple random sampling which explains the comparison between the variables of the GeneXpert and Ziehl-Neelsen examination results in pulmonary tuberculosis patients using a cross-sectional approach, namely where data is obtained from the pulmonary TB population at Hospital X Yogyakarta from 2021-2024 according to the variables studied. Based on the TCM results which are greater than the BTA results, these results indicate a significant difference between the GeneXpert method and the Ziehl-Neelsen method in terms of examination results. The results obtained from the examination using the TCM/GeneXpert and Ziehl-Neelsen methods show a higher detection rate of GeneXpert than Ziehl-Neelsen in identifying TB cases.
Identifikasi Bakteri Staphylococcus Aureus pada Spons Make-Up di Kalangan Mahasiswa Putri Maharani; Wiwit Probowati; Widaninggar Rahma Putri
Sehat Rakyat: Jurnal Kesehatan Masyarakat Vol. 5 No. 3 (2026): Agustus 2026
Publisher : Yayasan Pendidikan Penelitian Pengabdian Algero

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54259/sehatrakyat.v5i3.7918

Abstract

Makeup sponges are porous cosmetic application tools that are reused and can potentially become a breeding ground for microorganisms if not cleaned regularly. One bacterium that can contaminate makeup sponges is Staphylococcus aureus, which are knew to do play with a role in various skin also soft tissue infections. This reseach was purposelly to identifying the presence of S. aureus towards makeup sponges used by college students. The study employed a quantitative experimental method using purposive sampling on 30 samples of makeup sponges that had been used for at least 1–2 months. Samples were grouped based on washing frequency: frequently washed, rarely washed, and never washed. Bacterial identification was performed via inoculation on Mannitol Salt Agar (MSA) medium, followed by subculturing, Gram staining, catalase testing, and coagulase testing. The results of the study showed colony growth on MSA medium, indicated by a yellow discoloration of the medium. Gram staining revealed coccus-shaped cells arranged in clusters and stained purple, indicating the characteristics of Gram-positive bacteria. The catalase and coagulase tests yielded positive results, confirming the presence of Staphylococcus aureus. Of the 30 samples examined, 7 positive samples were found in the group of sponges that were rarely washed, 6 positive samples in the group that were never washed, and 3 positive samples in the group that were frequently washed. These findings indicate that S. aureus contamination is more common in sponges that are rarely or never washed compared to those cleaned regularly.
Co-Authors Andini Bilindade Putri Maharani Wiwit Probowati