Articles
<![CDATA[Callus Induction and Differentiation on Melon From In Vitro Culture with The Addition of Indole Acetic Acid and Benzyl Amino Purine Growth Regulator ]]>
<![CDATA[Probowati, Wiwit]]>;
<![CDATA[Daryono, Budi Setiadi]]>
<![CDATA[ PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 6, No 1 (2018) ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Yogyakarta ]]>
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DOI: 10.18196/pt.2018.076.15-21
<![CDATA[Melon plants (Cucumis melo L.) are susceptible to disease attacks primarily caused by viruses. One of the viruses often attacking melon plants is Cucumber mosaic virus (CMV). Previous studies have shown that melon callus produced from in vitro culture is detected to have CMV resistance gene (Creb-2). However, the trait of resistance to CMV in that melon (F2) is still segregated. To know the stability of the Creb-2 gene, further research is needed to detect the gene in the differentiated callus. This study aims to callus induce and callus differentiate melon. Firstly, melon seeds were grown on ¼ MS medium with addition of 2 mg / L 2.4-Dichlorophenoxyacetate and 1 mg / L BAP (Benzyl Amino Purine) to grow callus. Secondly, the callus was differentiated in MS medium with the addition of 2,4-D , BAP and IAA in different doses. 0 mg / L; 0.01 mg / L 2.4-D and 0.1 mg / L BAP, and MS medium with 0.01 mg / L; 0.1 mg / L; 1 mg / L IAA (Iodole Acetic Acid) and 0.1 mg / L BAP. The results showed that callus was successfully differentiated into root and it grew optimally in MS medium with 0.01 mg / L 2.4-D: 0.1 mg / L BAP and 1 mg / L IAA: 0.1 mg / L BAP . Therefore the roots can be used as isolation samples and DNA amplification to detect stability of the Creb-2 gene.]]>
<![CDATA[Efektivitas Pupuk Cair Pseudomonas fluorescens Agensia Pengendali Hayati Terhadap Penyakit Mosaik Tanaman Kakao ]]>
<![CDATA[Probowati, Wiwit]]>;
<![CDATA[Nugraheni, Ika Afifah]]>;
<![CDATA[Aryani, Titin]]>
<![CDATA[ Bioeksperimen: Jurnal Penelitian Biologi Vol 7, No 1: Maret 2021 ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surakarta ]]>
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DOI: 10.23917/bioeksperimen.v7i1.10245
<![CDATA[Cocoa is a strategic commodity for plantation in Indonesia. However, pest and disease infections are inevitably common constrains for the cocoa cultivators. Cocoa mosaic disease is one of disease which is cause cocoa trees suffering. Pseudomonas fluorescens are obligate aerobic bacteria that are very valuable for agricultural technology. The bacteria also protects plants from pathogens infection by producing secondary metabolites that kill bacteria and other fungi. The bacteria can used as safe and environmentally friendly biological control agents. This study aims to determine P. fluorescens liquid fertilizer formulation and its effectiveness in suppressing mosaic disease in cocoa plants. The research method was carried out by making a liquid formula from P. fluorescens and then applying it to cocoa plants that were attacked by mosaic disease. Bacterian isolates grown in conch broth medium were incubated for 3 days. Then the fertilizer formula was applied to mosaic symptomatic cocoa plants with fertilizer concentrations of 25%, 50% and 75% compared to spraying using chemical insecticides. The results showed that the liquid formula concentration of 75% can suppress the most effective mosaic symptoms and the optimal growth of cocoa plants for 7 treatments.]]>
<![CDATA[Callus Induction and Differentiation on Melon From In Vitro Culture with The Addition of Indole Acetic Acid and Benzyl Amino Purine Growth Regulator ]]>
<![CDATA[Probowati, Wiwit]]>;
<![CDATA[Daryono, Budi Setiadi]]>
<![CDATA[ PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 6, No 1 (2018) ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Yogyakarta ]]>
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DOI: 10.18196/pt.2018.076.15-21
<![CDATA[Melon plants (Cucumis melo L.) are susceptible to disease attacks primarily caused by viruses. One of the viruses often attacking melon plants is Cucumber mosaic virus (CMV). Previous studies have shown that melon callus produced from in vitro culture is detected to have CMV resistance gene (Creb-2). However, the trait of resistance to CMV in that melon (F2) is still segregated. To know the stability of the Creb-2 gene, further research is needed to detect the gene in the differentiated callus. This study aims to callus induce and callus differentiate melon. Firstly, melon seeds were grown on ¼ MS medium with addition of 2 mg / L 2.4-Dichlorophenoxyacetate and 1 mg / L BAP (Benzyl Amino Purine) to grow callus. Secondly, the callus was differentiated in MS medium with the addition of 2,4-D , BAP and IAA in different doses. 0 mg / L; 0.01 mg / L 2.4-D and 0.1 mg / L BAP, and MS medium with 0.01 mg / L; 0.1 mg / L; 1 mg / L IAA (Iodole Acetic Acid) and 0.1 mg / L BAP. The results showed that callus was successfully differentiated into root and it grew optimally in MS medium with 0.01 mg / L 2.4-D: 0.1 mg / L BAP and 1 mg / L IAA: 0.1 mg / L BAP . Therefore the roots can be used as isolation samples and DNA amplification to detect stability of the Creb-2 gene.]]>
<![CDATA[Pemberdayaan Perempuan Dalam Produksi Masker Dan Penerapan Disiplin Memakai Masker Bagi Pekerja Harian Dalam Penanganan Wabah Covid-19 ]]>
<![CDATA[Wiwit Probowati]]>;
<![CDATA[Nosa Septiana Anindhita]]>
<![CDATA[ Jurnal Pengabdian UntukMu NegeRI Vol 5 No 1 (2021): Pengabdian Untuk Mu negeRI ]]>
Publisher : <![CDATA[LPPM UMRI ]]>
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DOI: 10.37859/jpumri.v5i1.2150
<![CDATA[The Covid-19 outbreak that hit globally had amajor impact on economic activity in developing countries, especially Indonesia. In order to continue carrying out daily activities, especially working, it is necessary to comply with health protocol. Health protocols when doing activities include wearing masks, washing hands frequently with soap, avoiding crowds and maintaining a minumum distance of 1.5 meters from each other. The need for masks that are currentky not fulfilled by daily workers or laborers in Margomulyo village, Seyegan sub-district, Sleman regency, Province of Yogyakarta is primary need while working. The importance of education regarding the dangers of transmission of the Corona Virus or SARS-Cov-2 needs to be done to prevent the faster transmission of the virus. Therefore, the organization group ‘Aisyiyah in collaboration with Universitas ‘Aisyiyah Yogyakarta, empowers women tailors in neighborhood to produce cloth masks. Furthermore, these mask were distributed to daily workers in the Mrgomulyo village environment who had high mobility and had ahigh potential to transmit the Corona virus.]]>
<![CDATA[Indeks Mitosis dan Jumlah Kromosom Kentang Hitam (Coleus tuberosus) ]]>
<![CDATA[Wiwit Probowati]]>;
<![CDATA[Alifiani Hikmah Putranti]]>
<![CDATA[ Vegetalika Vol 9, No 4 (2020) ]]>
Publisher : <![CDATA[Universitas Gadjah Mada ]]>
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DOI: 10.22146/veg.50565
<![CDATA[Kentang hitam (Coleus tuberosus) merupakan jenis kentang yang paling langka dari jenis kentang lainnya. Tanaman kentang hitam yang berasal dari Afrika Barat ini memiliki umbi yang tergolong kecil dan daging umbi berwarna hitam. Kurang optimalnya pemanfaatan kentang hitam sebagai sumber karbohidrat non beras sejalan dengan berkurangnya penelitian dan pengembangan komoditas tersebut. Pemanfaatan kentang hitam sebagai sumber karbohidrat masih sangat kurang sehingga ketertarikan riset dan pengembangan komoditas kentang hitam juga rendah. Tanaman Solanaceae merupakan tanaman yang memiliki variasi morfologi dan jumlah kromosom terbesar. Tujuan penelitian ini adalah untuk mengetahui waktu mitosis aktif dan jumlah kromosom kentang hitam. Sehingga hal ini akan menambah data base bank genetik. Penelitian dilakukan pada bulan Mei 2019 hingga Desember 2019 di Laboratorium Terpadu, Universitas ‘Aisyiyah Yogyakarta. Bahan tanaman yang digunakan adalah ujung akar kentang hitam yang dipreparasi kromosomnya menggunakan metode squashing. Preparasi kromosom dimulai dari pukul 06.00 WIB sampai pukul 13.00 WIB dengan interval waktu preparasi kromosom setiap 30 menit dengan 3 ulangan. Hasil penelitian didapatkan bahwa waktu mitosis kentang hitam (Coleus tuberosus) berkisar antara pukul 07.00-11.00 WIB, sedangkan fase prometafase ditemukan pada pukul 07.30 WIB. Jumlah kromosom diploid (2n) kentang hitam adalah 32 kromosom.]]>
<![CDATA[Efektivitas Pupuk Cair Pseudomonas fluorescens Agensia Pengendali Hayati Terhadap Penyakit Mosaik Tanaman Kakao ]]>
<![CDATA[Wiwit Probowati]]>;
<![CDATA[Ika Afifah Nugraheni]]>;
<![CDATA[Titin Aryani]]>
<![CDATA[ Bioeksperimen: Jurnal Penelitian Biologi Vol 7, No 1: March 2021 ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surakarta ]]>
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DOI: 10.23917/bioeksperimen.v7i1.10245
<![CDATA[Cocoa is a strategic commodity for plantation in Indonesia. However, pest and disease infections are inevitably common constrains for the cocoa cultivators. Cocoa mosaic disease is one of disease which is cause cocoa trees suffering. Pseudomonas fluorescens are obligate aerobic bacteria that are very valuable for agricultural technology. The bacteria also protects plants from pathogens infection by producing secondary metabolites that kill bacteria and other fungi. The bacteria can used as safe and environmentally friendly biological control agents. This study aims to determine P. fluorescens liquid fertilizer formulation and its effectiveness in suppressing mosaic disease in cocoa plants. The research method was carried out by making a liquid formula from P. fluorescens and then applying it to cocoa plants that were attacked by mosaic disease. Bacterian isolates grown in conch broth medium were incubated for 3 days. Then the fertilizer formula was applied to mosaic symptomatic cocoa plants with fertilizer concentrations of 25%, 50% and 75% compared to spraying using chemical insecticides. The results showed that the liquid formula concentration of 75% can suppress the most effective mosaic symptoms and the optimal growth of cocoa plants for 7 treatments.]]>
<![CDATA[Pembentukan Komunitas Masyarakat Pembuat Media Tanam dari Sampah Plastik Kresek ]]>
<![CDATA[Wiwit Probowati]]>;
<![CDATA[Ika Afifah Nugraheni]]>;
<![CDATA[Suryadi Suryadi]]>
<![CDATA[ PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 5 No 2 (2020): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat ]]>
Publisher : <![CDATA[Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya ]]>
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DOI: 10.33084/pengabdianmu.v5i2.1067
<![CDATA[The focus of the community service program is the formation of communities that are committed to processing plastic rubbish materials into planting media (mediokres). Mediokres is a product of Sayidan Agro Media’s organizational innovation in Sleman, D.I. Yogyakarta, which is able to utilize plastic bag rubbish at the Final Disposal Site becomes a planting medium to replace soil. Mediokres has been widely marketed in Indonesia However, in the production process of mediokres, there is the obstacle, one of which is the supply of mediokres products which is slow compared to the large consumer demand. Therefore the formed mediokres community is able to help meet the mediokres stock both for ordering and stocking every month. So that consumer demand is fulfilled.]]>
<![CDATA[Tangguh Hadapi Covid-19 dengan Budidaya Tanaman Obat Keluarga di Desa Margomulyo, Seyegan, Sleman ]]>
<![CDATA[Wiwit Probowati]]>;
<![CDATA[Fuad Gandhi Torizal]]>;
<![CDATA[Sunartiyah Sunartiyah]]>
<![CDATA[ PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 7 No Special-1 (2022): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat ]]>
Publisher : <![CDATA[Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya ]]>
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DOI: 10.33084/pengabdianmu.v7iSpecial-1.2692
<![CDATA[During the Covid-19 outbreak, it is essential to maintain faith and a happy soul; it is also necessary to maintain our health immunity. The body's immunity can be supported by consuming healthy food and a balanced diet. Consumption of additional supplements from chemical medicine cause side effects if consumed overdoses. By utilizing the land yard for the cultivation of herbal plants, it can be used as an alternative solution to reduce the consumption of chemical drugs, in line with the problems faced by the program partner nursery, which has an extensive collection of medicinal plants but does not yet know the efficacy of each type of plant. The community service program during the pandemic was carried out through limited counseling to 30 health cadres of Margomulyo village. The method used in this activity is public education through lectures, questions, and discussions. Participants were also given a pocketbook "List of Herbal Plants and their benefits and also given plant seeds. Participants' level of knowledge was measured using a questionnaire given before and after the lecture. From the results, it can be seen that there is an increase in the understanding of the participant's post-test scores compared to pre-test scores. Participants' increased knowledge about family medicinal plants was expected to share the knowledge with the broader community and apply the cultivation of herbal plants inland around the house.]]>
<![CDATA[Pelatihan Pembuatan Nata de coco di Perkebunan Kelapa Desa Margomulyo Kecamatan Seyegan Kabupaten Sleman ]]>
<![CDATA[Wiwit Probowati]]>;
<![CDATA[Aulia Ulfah Mu’awanah]]>
<![CDATA[ Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol 5, No 1 (2021): Vol 5, No 1 (2021) ]]>
Publisher : <![CDATA[Yogyakarta State University ]]>
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DOI: 10.21831/jpmmp.v5i1.28419
<![CDATA[Coconut plants are very potential commodities, all parts of the coconut plant are beneficial to the needs of human life. All parts of coconuts are not wasted and can be used to produce industrial products. The partners are coconut farmers who sell peeled coconut and coconut flesh. Farmers in Margomulyo village have difficulty in handling the waste of untreated coconut water. Sometimes the coconut water causes inundated land without being used. Sometimes when the coconut water is not directly absorbed in the ground, it will cause bad odor in the environment. Therefore, the use of coconut water is a way to optimize the use of coconut fruit. The content of sucrose sugar in coconut water will be used by Acetobacter xylinum as a source of energy, as well as a carbon source to form metabolite compounds such as cellulose that form Nata de Coco. In conclusion, Nata de coco making training in Margomulyo village gave positive response for the community to establish business unit. The barrier to the implementation of this activity was that there was no solution for handling waste production of Nata de coco of which aroma was quite disturbing. Thus, the implementation of nata de coco waste treatment program will be overcomed in further implementation of community service program which will be held in the next period]]>
<![CDATA[FORMULASI PUPUK CAIR Pseudomonas fluorescens SEBAGAI AGENSIA PENGENDALI HAYATI PENYAKIT MOSAIK TANAMAN KAKAO ]]>
<![CDATA[Wiwit Probowati]]>;
<![CDATA[Pilar Rosatria Firyalunfah]]>;
<![CDATA[Wahyuni Wulansari]]>
<![CDATA[ JURNAL VIGOR Vol 5, No 2 (2020): VIGOR: JURNAL ILMU PERTANIAN TROPIKA DAN SUBTROPIKA ]]>
Publisher : <![CDATA[Universitas Tidar ]]>
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DOI: 10.31002/vigor.v5i2.3072
<![CDATA[Cocoa is a strategic commodity for plantation in Indonesia. However, pest and disease infections are inevitably common constrains for the cocoa cultivators. Cocoa mosaic disease is one of disease which is cause cocoa trees suffering. Pseudomonas fluorescens are obligate aerobic bacteria that are very valuable for agricultural technology. The bacteria also protect plants from pathogens infection by producing secondary metabolites that kill bacteria and other fungi. The bacteria can use as safe and environmentally friendly biological control agents. This study aims to determine P. fluorescens liquid fertilizer formulation and its effectiveness in suppressing mosaic disease in cocoa plants. The research method was carried out by making a liquid formula from P. fluorescens and then applying it to cocoa plants that were attacked by mosaic disease. Bacteria isolates grown in conch broth medium were incubated for 3 days. Then the fertilizer formula was applied to mosaic symptomatic cocoa plants with fertilizer concentrations of 25%, 50% and 75% compared to spraying using chemical insecticides. The results showed that the liquid formula concentration of 75% can suppress the most effective mosaic symptoms and the optimal growth of cocoa plants for 7 treatments.. Keywords: Cocoa, Formulation, mosaic disease, Pseudomonas fluorescens]]>
