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All Journal Biodiversitas Journal of Biological Diversity
NOVIK NURHIDAYAT
Bidang Mikrobiologi, Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI), Bogor 16002.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Use of local isolate of Monascus purpureus for reducing blood cholesterol in Sprague Dawley rat ERNAWATI KASIM; YETY KURNIAWATI; NOVIK NURHIDAYAT
Biodiversitas Journal of Biological Diversity Vol. 7 No. 2 (2006)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d070206

Abstract

Research using local isolate of M. purpureus to reduce the blood cholesterol total on the white rats of Sprague Dawley strain had been done. The research proposed was to obtain the best doses of fermented rice contain lovastatin to reduce the blood cholesterol content of the white rats Sprague Dawley strain. The research used 42 male rats which was devided into 6 treatments based on the food and fermented rice doses. Each rat was 2 months and 250 gram in weight. The cholesterol food and the standard food given everyday for 20 gram/day/rat. The blood rats was measured the content of cholesterol every seven days for 21 days. The result showed that the lovastatin of the fermented rice could reduce 20,78% the blood cholesterol total of the white rats Sprague Dawley strain. © 2006 Jurusan Biologi FMIPA UNS SurakartaKey words: Monascus purpureus , kolesterol, tikus putih galur Sprague Dawley
Viability of encapsulated Lactobacillus sp. Mar 8 EVI TRIANA; EKO YULIANTO; NOVIK NURHIDAYAT
Biodiversitas Journal of Biological Diversity Vol. 7 No. 2 (2006)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d070204

Abstract

Lactobacillus sp. Mar 8 had advantages as probiotic digestive system cholesterol lowering Lactobacillus. Applying in industry, particular processing technique is necessary for gaining product that ready for marketing and consuming. Spray drying is common technique using in various food processing. High processing temperature, 100-200oC, for 3-10 second become the barrier because cells were under extreme temperature stress. Therefore, encapsulate was needed to protect the cells from those extreme conditions. Viability and survival rate of encapsulated Lactobacillus sp. Mar 8 have been investigated. The result showed that Lactobacillus sp. Mar 8 that was encapsulated by 10% skim milk has higher viability than those by 5% skim milk, namely 72.37% and 51.69% respectively. Survival rate of encapsulated Lactobacillus cells will come to zero in 41.28 years. Therefore, encapsulated Lactobacillus sp. Mar 8 may use as probiotic agent. © 2006 Jurusan Biologi FMIPA UNS SurakartaKey words: Lactobacillus sp. Mar 8, viability, spray drying, encapsulation, survival rate.
Pigment and Lovastatin content on the Red Rice cultivar Bah Butong and BP 1804 IF 9 which Fermented by Monascus purpureus Jmba ERNAWATI KASIM; NANDANG SUHARNA; NOVIK NURHIDAYAT
Biodiversitas Journal of Biological Diversity Vol. 7 No. 1 (2006)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d070103

Abstract

Research on the red rice fermented by Monascus purpureus had been done. The rice consisted of 2 cultivars such as Bah Butong and BP 1804 IF 9. The aim of the research was to know the content of the pigment and lovastatin of the fermentation result/ angkak. Angkak was powdered by using blender. To measure the content of pigment, the powder was extracted by methanol. By using spectrophotometer the content of the pigment could be measured with 390 nm wave lengths for yellow pigment and 500 nm for the red pigment. For lovastatin the powder was extracted by acetonitrile and H2SO4. By using HPLC the content of lovastatin could be measured. The results showed that the highest pigment content for yellow pigment was on the PB 1804 IF 9 cultivar, and red pigment was on the Bah Butong cultivar. The highest lovastatin content was on the BP 1804 IF 9.Key words: Monascus purpureus, red rice, pigment, lovastatin.
Co-Authors EKO YULIANTO ERNAWATI KASIM EVI TRIANA NANDANG SUHARNA YETY KURNIAWATI