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Subterranean Types of Endogone Spores in Jobolarangan Forest SHANTI LISTYAWATI; DEWI HANDADARI; BUDI SARYANTO; BAYU IRAWAN; DIAH DWI HANDAYANI
Biodiversitas Journal of Biological Diversity Vol. 2 No. 2 (2001)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d020204

Abstract

Endogone spores are spores produced by Endogone genus of VAM fungi, which are abudant in soil. The objective of the research was to assess the types of Endogone spores found in the soil of Jobolarangan forest. Soil samples were taken based on the dominan vegetation, identification and spores screening were then done to the sample. The result research indicated that there were 5 types of spores, namely: white reticulate, yellow vacuolate, red brown laminate, honey colored sessile, and crenulate.© 2001 Jurusan Biologi FMIPA UNS SurakartaKey words: Endogone spores, spore types, Jobolarangan forest
A Chemotaxonomic Study in the Genus Zingiber MARSUSI MARSUSI; AHMAD DWI SETYAWAN; SHANTI LISTYAWATI
Biodiversitas Journal of Biological Diversity Vol. 2 No. 1 (2001)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d020102

Abstract

Zingiber is profitable for spice, ingredients, medicine and garnishing plant (Purseglove, 1972). The systematic ofZingiber (and other Zingiberaceae) has been argued among the authors, because they commonly use the morphological and the anatomical characters, that they obtain limited data. The chemical constituents of volatile oils are one of the most prospective characters for taxonomy of Zingiber. This research is objected to find out (1) the constituents of volatile oils (2) the number and the type of compounds composing volatile and (3) the genetic relationship. This research is done in the laboratory. The data seeking covers, i.e. (1) water distillation (Guenther, 1948; Anon, 1977), (2) extraction (Anon, 1977; Harborne, 1984), and gas chromatography (Mc Nair & Bonelli, 1968;Pramono, 1988). Dendrogram is arranged referring to Sokal & Sneath (1963), and the association coefficient degrees are determined referring to Pielou (1984). The rhizomes are gathered from Bogor Botanical Garden and from around Surakarta. There are seven achieved species, namely Z. amaricans Nor., Z. aromaticum Val., Z. cassumunar Roxb.,Z. gramineum Bl., Z. officinale Roxb., Z. ottensii Val., and Z. zerumbet (L.) J.E. Smith. Every species is identified referring to manuals of Backer & Bakhuizen van den Brink (1968), Holttum (1950) and Burkill (1935). The volatile contents of seven species subsequently are 4.67% (ml/100gr.), 5.00%, 6.33%, 0.20%, 6.67%, 4.29% and 6.00%. The numbers of composing volatile compounds subsequently are 30, 26, 37, 44, 29, 29 and 29. The genetic relationships of seven species are Z. amaricans, Z. aromaticum and Z. zerumbet joint at similarity index of 90, and it is followed by Z. ottensii at similarity index of 85. Then those five species join with Z. cassumunar and Z. gramineum at similarity index of 60. The last is the joining of Z. officinale to those six species at similarity index of 55.© 2001 Jurusan Biologi FMIPA UNS SurakartaKeywords: chemotaxonomy, volatile oils, Zingiber.
Source of contaminant microorganisms in in vitro culture at Sub lab. Biology, Central Laboratory of Mathematics and Sciences, Sebelas Maret University ARI SUSILOWATI; SHANTI LISTYAWATI
Biodiversitas Journal of Biological Diversity Vol. 2 No. 1 (2001)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d020105

Abstract

The objectives of the research were to know the species and the most dominant microorganisms that become a source of contamination in in vitro culture at Sub lab Biology, central laboratory of Sebelas Maret University. As in many general laboratory, there were many microorganisms that able to contaminate in vitro culture coming from air, dusts, or from the contaminated experimental materials such as plants or fruits. A qualitative descriptive method was used in the research, involving many steps of making pure culture and identification of microorganisms macroscopically or microscopically. In the research found six microorganisms potentially contaminate in vitro culture, that are generally from groups of fungi (mold), such as Mucor, Rhizopus, Cladosporium, Aspergillus, Dictyostelium and Saccharomyces. Mucor and Rhizopus were the most contaminants present in all contaminated in vitro culture.© 2001 Jurusan Biologi FMIPA UNS SurakartaKey Words: in vitro culture, contamination, fungi