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APRILIA SUFI SUBIASTUTI
Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sleman 55281, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Detection and identification of Begomovirus infecting Cucurbitaceae and Solanaceae in Yogyakarta, Indonesia APRILIA SUFI SUBIASTUTI; SEDYO HARTONO; BUDI SETIADI DARYONO
Biodiversitas Journal of Biological Diversity Vol. 20 No. 3 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200318

Abstract

Abstract. Subiastuti AS, Hartono S, Daryono BS. 2019. Detection and Identification of Begomovirus infecting Cucurbitaceae and Solanaceae in Yogyakarta, Indonesia. Biodiversitas 20: 738-744. Begomovirus genome has high plasticity that led to evolve rapidly. Begomovirus is one of a remarkably successful group of emerging viruses as the results from combination of many factors. Planting systems in Indonesia which often overlapping two or more plant species in one land has high possibility for occurring mixed infection. It is also suggested has high contribution to increase Begomovirus diversity. The aim of this research is to do preliminary identification of Begomovirus infected-Solanaceae and Cucurbitaceae in Yogyakarta based core coat protein (CP) gene sequence. A total of 50 melon, 50 chili, 30 eggplants, and 30 watermelon samples which showed Begomovirus symptoms were observed from several fields in Yogyakarta and Purworejo, Indonesia during 2016. Almost 90% of infected samples for each plant were tested by PCR and showed positive for Begomovirus. Based on coat protein (CP) gene nucleotide sequence identity, Begomovirus infected Solanaceae in Indonesia has close relationship with Pepper yellow leaf curl Indonesia virus (PepYLCIV) and Tomato yellow leaf curl Kanchanaburi virus (TYLCVKaV), while in Cucurbitaceae has close relationship with Squash leaf curl China virus (SLCCV) Tomato leaf curl New Delhi virus (ToLCNDV). All collected isolates showed highest sequence identity with isolates from South-East Asia and China. However, further analysis that including full genome characterization is still needed to explain Begomovirus evolution in Indonesia.
Phenotypic and genetic stability of new Indonesian melon cultivar (Cucumis melo L. ‘Melonia’) based on ISSR markers BUDI SETIADI DARYONO; APRILIA SUFI SUBIASTUTI; ARVITA FATMADANNI; DIAN SARTIKA
Biodiversitas Journal of Biological Diversity Vol. 20 No. 4 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200419

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Abstract. Daryono BS, Subiastuti AS, Fatmadanni A, Sartika D. 2019. Phenotypic and genetic stability of new Indonesian melon cultivar (Cucumis melo L. ‘Melonia’) based on ISSR markers. Biodiversitas 20: 1069-1075. Cucumis melo L. ‘Melonia’ was new Indonesian melon cultivars produced by segregation of Meloni cultivar. The Meloni cultivar has a yellowish-skinned color, orange flesh fruit, and sweet taste. The stability of phenotype and genotype characters of this new cultivars were assessed using 27 morphological traits and 4 ISSR primers, respectively. Phenotype characters in F2 and F3 populations have been stable on 25 of 27 morphological traits used, except in weight of seed cavity and color of skin fruit. The 4 ISSR markers were produced 41 fragments, contained 28 monomorphic DNA bands and 13 polymorphic DNA bands. All of DNA bands were scored and used for genetic similarity analysis using MVSP 3.1A Program. The highest genetic variation was produced by UBC-808 primer with a polymorphic percentage of 38.46%. All of ‘Melonia’ populations were clustered together with 100% similarity percentage.  Compared to other cultivars, ‘Melonia’ had high similarity with ‘Meloni’ in 87.8%, meanwhile, ‘Melonia’ and Meloni cultivars had relationship with Melona at a similarity value of 70.7%.
Co-Authors ARVITA FATMADANNI BUDI SETIADI DARYONO DIAN SARTIKA SEDYO HARTONO