Biodiversitas Journal of Biological Diversity
Vol. 20 No. 3 (2019)

Detection and identification of Begomovirus infecting Cucurbitaceae and Solanaceae in Yogyakarta, Indonesia

APRILIA SUFI SUBIASTUTI (Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sleman 55281, Yogyakarta, Indonesia)
SEDYO HARTONO (Department of Pest and Plant Disease, Faculty of Agriculture, Universitas Gadjah Mada. Jl. Flora, Bulaksumur, Sleman 55281, Yogyakarta, Indonesia)
BUDI SETIADI DARYONO (Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sleman 55281, Yogyakarta, Indonesia)



Article Info

Publish Date
22 Feb 2019

Abstract

Abstract. Subiastuti AS, Hartono S, Daryono BS. 2019. Detection and Identification of Begomovirus infecting Cucurbitaceae and Solanaceae in Yogyakarta, Indonesia. Biodiversitas 20: 738-744. Begomovirus genome has high plasticity that led to evolve rapidly. Begomovirus is one of a remarkably successful group of emerging viruses as the results from combination of many factors. Planting systems in Indonesia which often overlapping two or more plant species in one land has high possibility for occurring mixed infection. It is also suggested has high contribution to increase Begomovirus diversity. The aim of this research is to do preliminary identification of Begomovirus infected-Solanaceae and Cucurbitaceae in Yogyakarta based core coat protein (CP) gene sequence. A total of 50 melon, 50 chili, 30 eggplants, and 30 watermelon samples which showed Begomovirus symptoms were observed from several fields in Yogyakarta and Purworejo, Indonesia during 2016. Almost 90% of infected samples for each plant were tested by PCR and showed positive for Begomovirus. Based on coat protein (CP) gene nucleotide sequence identity, Begomovirus infected Solanaceae in Indonesia has close relationship with Pepper yellow leaf curl Indonesia virus (PepYLCIV) and Tomato yellow leaf curl Kanchanaburi virus (TYLCVKaV), while in Cucurbitaceae has close relationship with Squash leaf curl China virus (SLCCV) Tomato leaf curl New Delhi virus (ToLCNDV). All collected isolates showed highest sequence identity with isolates from South-East Asia and China. However, further analysis that including full genome characterization is still needed to explain Begomovirus evolution in Indonesia.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...