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All Journal Biodiversitas Journal of Biological Diversity
TRIWIBOWO YUWONO
Department of Agricultural Microbiology, Faculty of Agriculture, Universitas Gadjah Mada. Jl. Flora, Bulaksumur, Sleman 55281, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Antimicrobial activities and phylogenetic study of bacteria associated with Cyperus rotundus rhizosphere from Cemoro Sewu Plateau, Indonesia AMBARWATI AMBARWATI; SUBAGUS WAHYUONO; SUKARTI MOELJOPAWIRO; LANGKAH SEMBIRING; TRIWIBOWO YUWONO
Biodiversitas Journal of Biological Diversity Vol. 20 No. 8 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200814

Abstract

Abstract. Ambarwati A, Wahyuono S, Moeljopawiro S, Sembiring L, Yuwono T. 2019. Antimicrobial activities and phylogenetic study of bacteria associated with Cyperus rotundus rhizosphere from Cemoro Sewu Plateau, Indonesia. Biodiversitas 20: 2206-2212. A study has been conducted to investigate the activity of bacteria isolated from the rhizosphere of Purple Nut Sedge (Cyperus rotundus) from Cemoro Sewu Plateu, Indonesia as antimicrobial agent and the molecular relatedness between the isolates with other bacteria based on phylogenetic tree analysis. A total of six bacteria were obtained and tested for their capability to inhibit the growth of test organisms using agar block method and characterized molecularly using 16S rDNA sequence analysis. It was observed that the six isolates demonstrated the ability to inhibit at least one of four test microorganisms growth, with a diameter of inhibition zone ranging from 8-35 mm. The CRC32 isolate was the best isolate in inhibiting Staphylococcus aureus ATCC 25923 with a diameter inhibition zone of 26 mm (strong), while isolate CRA8 was the best anti-Candida (showing 35 mm of inhibition zone). The phylogenetic tree analysis resulted in two genera, four isolates belonging to Bacillus, while the other two isolates were known related to Paenibacillus genus. BLAST analysis on the CRC 32 isolate revealed that it is closely related with Paenibacillus peroriae strain 3763 with 99% sequence similarities and CRA8 isolates demonstrated the highest similarity with Paenibacillus sonchi strain X19-5 (97%). The results of this study demonstrated that the bacterial isolates from plant rhizosphere of Cemoro Sewu Plateau were the members of the Bacillus and Paenibacillus genera and demonstrated the potential as antimicrobial agent.
Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure Ambarwati Ambarwati; Subagus Wahyuono; Sukarti Moeljopawiro; Triwibowo Yuwono
Biodiversitas Journal of Biological Diversity Vol. 21 No. 7 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210763

Abstract

Abstract. Ambarwati A, Wahyuono S, Moeljopawiro S, Yuwono T. 2020. Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure. Biodiversitas 21: 3380-3390. The goal of the research was to determine the antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and predict their chemical structure of the bioactive compounds based on the AntiSMASH analysis of the whole genome sequencing. Streptomyces sp. CRB46 was isolated from Cyperus rotundus L. rhizosphere in the Cemoro Sewu highland, Indonesia (1920 meters above sea level). The results showed that the supernatant ethyl acetate (EAS) extract could inhibit 11 of 12 tested microorganisms with a diameter of the inhibition zone ranging from 17-35 mm (paper disc method). The supernatant extract was found more effective in inhibiting microorganisms than the ethyl acetate extract of pellets (EAP). The EAP extract was only able to inhibit the growth of seven tested microorganisms (16-26 mm). The results of LCMS analysis showed that crude extract of EAS consisted of 20 mixtures of organic compounds. Based on FTIR analysis, 16 functional groups were found in the EAS extracts. In addition, based on AntiSMASH analysis, it was also found that Streptomyces sp. CRB46 produced eight classes of bioactive compounds, but only six compounds have been identified as their chemical structures. It can, therefore, be concluded that Streptomyces sp. CRB46 has the potential to produce bioactive compounds that can be used for microbial infection treatment.
The potential of copper-resistant bacteria Acinetobacter sp. strain CN5 in decolorizing dyes WAHYU IRAWATI; REINHARD PINONTOAN; BELLEN MOURETTA; TRIWIBOWO YUWONO
Biodiversitas Journal of Biological Diversity Vol. 23 No. 2 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230212

Abstract

Abstract. Irawati W, Pinontoan R, Mouretta B, Yuwono T. 2021. The potential of copper-resistant bacteria Acinetobacter sp. strain CN5 in decolorizing dyes. Biodiversitas 23: 680-686. Bacteria with multi-resistance to copper and dyes may be employed in bioremediation of copper and dye waste more effectively than physical or chemical remediation. This study aims at determining the effect of the addition of various textile dyes and copper on the growth of Acinetobacter sp. CN5 and its ability to decolorize dyes. The dye resistance test was carried out by inoculating bacterial isolates into Luria Bertani media containing 50, 100, 150, 200, 250, 300, 350, 400, 450, and 500 ppm dyes. The twelve dyes used in the test were methylene blue, malachite green, congo red, mordant orange, reactive black, direct yellow, basic fuchsine, reactive orange, dispersion orange, remazol red, wantex yellow, and wantex red. The decolorization activity was analyzed by spectrophotometry at a 300-900 nm wavelength. The study results demonstrated that bacteria thrived in media containing 50 ppm of all dyes, except malachite green dye. Acinetobacter sp. CN5 was found to be resistant to up to 500 ppm methylene blue, basic fuchsine, and wantex red and resistant to congo red at 450 ppm. Acinetobacter sp. CN5 was also able to decolorize methylene blue, congo red, basic fuchsine, and red wantex by 57.64%, 53.17%, 91.37%, and 67.50%, respectively. The addition of 5 mM CuSO4 to the medium increased the ability of Acinetobacter sp. CN5 to decolorize the congo red from 57.64% to 82.58%.
Co-Authors AMBARWATI AMBARWATI Ambarwati Ambarwati BELLEN MOURETTA LANGKAH SEMBIRING REINHARD PINONTOAN SUBAGUS WAHYUONO Sukarti Moeljopawiro SUKARTI MOELJOPAWIRO WAHYU IRAWATI