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TRI ARDYATI
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Diversity and potency of indigenous yeast from some palm juices for bioethanol production Trianik Widyaningrum; SUHARJONO SUHARJONO; TRI ARDYATI; AULANNI’AM AULANNI’AM
Biodiversitas Journal of Biological Diversity Vol. 21 No. 1 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210139

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Abstract. Widyaningrum T, Suharjono S, Ardyati T, Aulanni’am A. 2020. Diversity and potency of indigenous yeast from some palm juices for bioethanol production. Biodiversitas 21: 318-325. Main energy source commonly used by the community comes from fossil energy, especially petroleum. The crisis of energy showed that Indonesia's fossil energy reserves are limited. Based on this fact it is important to develop alternative energy that environmentally friendly and sustain, especially bioethanol. The objective of this research was to diversity and potency of indigenous yeast from palm juice of Arenga pinnata Merr., Cocos nucifera L., Nypa fruticans Wurmb., and Borassus flabellifer L. for bioethanol production. Yeast from juice of the four species of palm was isolated using Yeast Malt Extract Agar (YMEA). Those isolates were screen base on ethanol production in coconut water media. The potential of yeast isolates was identified base on 18S DNA sequence similarity. The four potential isolates of yeast were A3A from A. pinnata, K1A from C. nucifera, N3E from N. fruticans, and S1A from B. flabellifer have highest production of ethanol are 8.6 %, 14.2 %, 9.0 %, and 9.2 % respectively. Four potential isolates of yeast were A3A and N3E, K1A, and S1A have relationship with Saccharomyces cerevisiae NRRL Y-12632T, Pichia manshurica IFO 10726T, and Candida tropicalis ATCC 20615 respectively.
Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia: - Siti Khotimah; Suharjono Suharjono; Tri Ardyati; Yulia Nuraini
Biodiversitas Journal of Biological Diversity Vol. 21 No. 5 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210538

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Abstract. Khotimah S, Suharjono, Ardyati T, Nurani Y. 2020. Isolation and identification of cellulolytic bacteria at fibric, hemic, and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia. Biodiversitas 21: 2103-2112. Cellulose degrading bacteria was one of the microbial removers of organic matter contained in the soil into simpler monomers so that it can be utilized by other organisms. The objective of the research was to obtain cellulose-degrading bacteria found on fibric, hemic, and sapric peat in forest and shrubs (oil palm). The bacteria were isolated by pour plate method on 1% CMC media. Selected isolates were assayed quantitatively based on the activity of cellulase enzyme, identified with 16S rDNA. The density of cellulolytic bacteria in the secondary forest peat of fibric, hemic, sapric were 2.1x103 cfu/g, 5.9x104 cfu/g, and 4.9x104 cfu/g whereas, in the area of shrubs/oil palm peat fibric, hemic and sapric 6.9x104 cfu/g, 8.4x104 cfu/g and 3.4x105 cfu/g respectively. There were 19 bacterial isolates that have clear zones around the colony as degradation of cellulose had highest ability to degrade cellulose with clear zones of 5-7 mm. The strain of SB1.1.1 showed highest activity of cellulase enzyme 11.17 U/mL, followed by HH3.1.1 strain and SB2.3 7.83 U/mL. Based on the phylogeny tree, strain SB1.1.1 and HH3.1.1 have the closest kinship relationship with Bacillus cereus with a kinship relationship of 100%, while SB2.3 has the closest kinship relationship with Bacillus stratosphericus with a relationship of 99.85 %.
Co-Authors AULANNI’AM AULANNI’AM Siti Khotimah SUHARJONO SUHARJONO Trianik Widyaningrum Yulia Nuraini