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All Journal Biodiversitas Journal of Biological Diversity
Abinawanto Abinawanto
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia. Jl. Lingkar UI, Depok 16424, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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The detection of plantaricin-encoding genes and their amino acid profiles in Lactobacillus plantarum AKK30 isolated from Indonesian native chicken ELISABETH LOVIAN ULI BASA; Abinawanto Abinawanto; A. SOPHIAN; H. JULENDRA; A. SOFYAN
Biodiversitas Journal of Biological Diversity Vol. 21 No. 12 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d211241

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Abstract. Elisabeth LUB, Abinawanto, Sophian A, Julendra H, Sofyan A. 2020. The detection of plantaricin-encoding genes and amino acid profile in Lactobacillus plantarum AKK30 isolated from Indonesian native chicken. Biodiversitas 21: 5792-5799. Plantaricin-encoding genes of lactic acid bacteria (LAB) Lactobacillus plantarum AKK30 which was isolated from Indonesian native chicken has been characterized using DNA sequencing technology. In order to substitute antibiotics for chickens and enhance probiotics in their gut, characterization of the indigenous probiotics in the chicken should be conducted. A potential probiotic in native chicken gut is Lactobacillus plantarum AKK30. Genes that harbor the bacteriocin biosynthesis of the strain were reported for the first time. The bacterium has diverse plantaricin genes, i.e., plnA, plnEF, plnJ, plnK and plnO. According to the dendrogram, the five plantaricin genes were closely related to plnA, plnE, plnF, plnJ, plnK and plnO genes from L. plantarum WCFS1, L. plantarum C11, and L. plantarum V90. Intriguingly, besides various plantaricin-encoding genes, another important probiotic property in L. plantarum AKK30 is cholesterol-lowering activity. Amino acid profiling also indicated proteolysis activity and probiotic potential, for instance, GABA production. Thus, L. plantarum AKK30 may be added in chicken broiler’s diet to provide probiotic benefits.
Short Communication: Effect of cryopreservation on ultrastructure and mitochondrial function of albino Pangasius catfish spermatozoa Uswatun Hasanah; ABINAWANTO ABINAWANTO; A ALIMUDDIN; Arief Boediono; Eni Kusrini
Biodiversitas Journal of Biological Diversity Vol. 21 No. 10 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d211008

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Abstract. Hasanah U, Binawanto, Alimuddin A, Boediono A, Kursini E. 2020. Short Communication: Effect of cryopreservation on ultrastructure and mitochondrial function of albino Pangasius catfish spermatozoa. Biodiversitas 21: 4524-4528. Cryopreservation techniques have been carried out on many endangered species and animals with unique characteristics. Successful cryopreservation techniques vary between species depending on various factors. The study used cryopreserved spermatozoa of the albino Pangasius catfish as samples. The cryopreserved spermatozoa were analyzed by its ultrastructure, functional mitochondria, and viability. The cryopreservation was performed using a combination of 10% methanol, skim milk, and fish ringer extender. A deep freezer is used for cryopreservation at -80 °C with a storage period of 14 days. Observations were made on fresh spermatozoa, post-equilibration spermatozoa, and frozen-thaw spermatozoa. This study found there were differences in ultrastructure and morphology in the three treatments. Fresh spermatozoa and post-equilibration spermatozoa appeared intact membrane, mitochondrial, and flagellar structures. In contrast, in frozen-thaw spermatozoa, there was damage to the cell membrane. The study showed different percentages of yields on functional mitochondria of fresh spermatozoa (98 ± 2%), spermatozoa post-equilibration (57 ± 7%), frozen-thaw spermatozoa (42 ± 3.21%). Cell viability showed that there were differences in viability of fresh spermatozoa and frozen-thaw (p <0.05), the results of fresh spermatozoa (92 ± 0.57%) spermatozoa post-equilibration (80 ± 3.51%), frozen-thaw spermatozoa (61 ± 2.30%).The study concluded that the spermatozoa cryopreservation affects the ultrastructure, mitochondrial function, and viability in albino Pangasius catfish spermatozoa.
Co-Authors A ALIMUDDIN A. SOFYAN A. SOPHIAN Arief Boediono ELISABETH LOVIAN ULI BASA Eni Kusrini H. JULENDRA Uswatun Hasanah