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All Journal Biodiversitas Journal of Biological Diversity
BUDI SETIADI DARYONO
Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sleman 55281, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Distribution, variation, and relationship of Curcuma soloensis Valeton in Java, Indonesia based on morphological characters MUHAMAD JALIL; AZIZ PURWANTORO; BUDI SETIADI DARYONO; Purnomo PURNOMO
Biodiversitas Journal of Biological Diversity Vol. 21 No. 8 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210856

Abstract

Abstract. Jalil M, Purwantoro A, Daryono BS, Purnomo. 2020. Distribution, variation, and relationship of Curcuma soloensis Valeton in Java, Indonesia based on morphological characters. Biodiversitas 21: 3867-3877. Curcuma soloensis Valeton (locally called temu genyeh) was a plant originating from Solomon Islands and was synonymous with Curcuma longa L. This plant was often considered to be turmeric (Curcuma longa Linn.) or temulawak (Curcuma zanthorrhiza Roxb.), because the rhizome is almost the same color. The purpose of this study was to determine the distribution, variation, and relationship of C. soloensis in Java, Indonesia. Retrieval of data with exploratory roaming methods in 12 districts/cities in Java Island as a center for planting medicinal plants. Morphological character observations were made on habit, rhizome, roots, tubers, leaves, pseudo-stems, and flowers. Morphological data were analyzed by descriptive and numerical methods. Analysis of grouping with Gower Coefficients because it uses 45 binary and multistate data. Principal Component Analysis (PCA) was performed to determine the role of each character in the grouping. Cluster analysis and PCA graphics were assisted with MVSP 3.1 software. The results of the study were obtained from 25 accessions of C. soloensis in East Java (Trenggalek, Pacitan, Ponorogo), Central Java (Wonogiri, Karanganyar, Magelang, Semarang), Yogyakarta (Yogyakarta City, Bantul, Gunungkidul), and West Java (Ciamis and Tasikmalaya). The variation of C. soloensis lies in habit, stem color, leaf shape, rhizome shape, rhizome flesh color, and tuber shape. The highest abundance percentage is in Pajangan, Tirtomoyo, and Tawangmangu. The dendrogram divides 32 OTUs into two clusters on the phenon line 0.617, namely cluster A (C. zanthorrhiza) and cluster B (C. soloensis and C. longa). PCA results showed that the characters that had the most role in grouping were leaf blade color, leaf blade length, rhizome shape, root color, rhizome taste, outer and inner rhizome flesh color.
Genetic stability of melon (Cucumis melo L. cv. Meloni) based on inter-simple sequence repeat and phenotypic characteristics ADIB FAKHRUDDIN YUSUF; WIKO ARIF WIBOWO; BUDI SETIADI DARYONO
Biodiversitas Journal of Biological Diversity Vol. 23 No. 6 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230631

Abstract

Abstract. Yusuf AF, Wibowo WA, Daryono BS. 2022. Genetic stability of melon (Cucumis melo L. cv. Meloni) based on inter-simple sequence repeat and phenotypic characteristics. Biodiversitas 23: 3042-3049.A new cultivar must have a character that follows the official criteria of distinctness, uniformity, and stability (DUS) as required for the grant of Plant Breeder Rights and official cultivar registration. Meloni is a new cultivar resulting from plant breeding by the Genetics and Breeding Laboratory of the Faculty of Biology, Universitas Gadjah Mada. Legality is needed in the form of plant variety protection (PVT)to protect the intellectual rights of researchers. Therefore, this study aims to identify morphological characters according to official criteria and validate molecular feelings by using molecular markers Inter-Simple Sequence Repeat. The results of morphological characterization showed that the distinctive character of the Meloni cultivar lies in the oval shape of the fruit, the skin of the fruit is creamy with a smooth texture without the net, and the flesh of the fruit is orange with a sweet taste. The differences in morphological characters of the Meloni cultivar cultivated at two locations differ in fruit weight, flesh thickness, fruit shelf life, and harvest age. Molecular identification resulted in a low similarity in the phenetic relationship, namely 35% against Sonya cultivars and 51% against Kirani and Kinanti cultivars. The Meloni cultivar's stability and uniformity analysis between the two locations (Bandung, West Java, and Sleman, Yogyakarta) yielded 76%. The difference in cultivation locations did not affect the level of stability and genetic uniformity of the Meloni cultivar.
Genetic diversity of eight maize (Zea mays) cultivars from East Nusa Tenggara (Indonesia) based on inter simple sequence repeat markers AHMAD YANI; ABDUL RAZAQ CHASANI; BUDI SETIADI DARYONO
Biodiversitas Journal of Biological Diversity Vol. 23 No. 8 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230833

Abstract

Abstract. Yani A, Chasani AR, Daryono BS. 2022. Genetic diversity of eight maize Zea mays L. cultivars from East Nusa Tenggara based on inter simple sequence repeat markers. Biodiversitas 23: 4124-4130. East Nusa Tenggara (NTT) is one of Indonesia's island that has many local maize cultivars. However, the existence of this local corn is threatened by the presence of high-yielding maize seeds. Therefore, characterization of local maize cultivars using molecular marker is needed to conserve the germplasm. This study aims to determine the genetic diversity of local maize cultivars (Zea maysL.) from  East Nusa Tenggara based on ISSR markers. This research used eight local maize cultivars (Watar Ohaq, Watar Puluq, Wata Bura, Wata Kuma, Wata Bunga, Pena Muti, Boti Pulut, and Latun Wara). Young leaves of maize were used for DNA isolation. Total genomic DNA was isolated using Genomic DNA Mini Kit for Plant (Geneaid). DNA was then amplified using 5 ISSR molecular markers (ISSR-807, ISSR-808, UBC-809, UBC-811, UBC-834). DNA band profiles were scored based on the presence or absence of bands. Genetic variability was analyzed based on the UPGMA method using the MVSP ver application. 3.1. Local maize from NTT has high genetic diversity (similarity index between 0.29-0.65). Local maize cultivars were separated into two main clusters at a similarity index of 0.43 and the grouping pattern was not influenced by the geographic location of local maize origin.
Assessment of maturity stage and stability of new Indonesian melon cultivar ‘Melona’ based on ISSR markers and morphological characteristics ADIB FAKHRUDDIN YUSUF; ADHESTYA ALFIANI; TANTRI AJENG SALMA SALSABILA; PRIMA SEKTI KUSNANDA; INA NUR HIDAYATI; BUDI SETIADI DARYONO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 1 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240137

Abstract

Abstract. Yusuf AF, Alfiyani A, Salsabila TAS, Kusnanda PS, Hidayati IN, Daryono BS. 2023. Assessment of maturity stage and stability of new Indonesian melon cultivar ‘Melona’ based on ISSR markers and morphological characteristics. Biodiversitas 24: 308-316. Market preferences for horticultural products determine the product’s selling value and absorption. One market criterion often used for the quality of horticultural products is color and maturity level. Color differences can provide a representation of the metabolites contained in a product. This study provides a standard for assessing melons' maturity level, which will be helpful as a benchmark for Indonesian consumer acceptance. In addition, this study also provides information on the stability of the character of the new cultivar 'Melona' from several generations of crosses to obtain superior cultivars. Assessment of maturity level based on color development in 'Melona' is divided into five stages. In the first and second stage, fruit was dominated by green color with Increasing fruit volume. The color change begins in the third stage. in the third stage and the following phases, there is an increase in the accumulation of beta-carotene and characterized by the dominance of orange instead of green. Maximum ripe of ‘Melona’ is characterized by strong yellow lobes, creamy-white interlobes, and orange flesh color. A molecular analysis employing four ISSR primers (UBC-807, UBC-808, UBC-811, and UBC-824) resulted in high uniformity and low genetic variation in the hybrid and parent lines genotype. Analysis resulted a total of 42 DNA fragments with only one polymorphic DNA fragment. The polymorphic DNA fragment was 2,300 bp in size and was found in the female accession 'Melona' and the hybrid 'Melona' based on the UBC-808 primer. The numerical phenetic relationship between male, female and hybrid accession melon cultivars is very close (above 97.6%) and indicated that the melona cultivars were stable.
Co-Authors ABDUL RAZAQ CHASANI ADHESTYA ALFIANI ADIB FAKHRUDDIN YUSUF AHMAD YANI AZIZ PURWANTORO INA NUR HIDAYATI MUHAMAD JALIL PRIMA SEKTI KUSNANDA PURNOMO PURNOMO TANTRI AJENG SALMA SALSABILA WIKO ARIF WIBOWO