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All Journal Biodiversitas Journal of Biological Diversity
RATNA SUSANDARINI
Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Short Communication: Composition of terpenoid compounds in essential oils extracted from stems of eight Piper species and their role in taxonomic relationships Laurentius Hartanto Nugroho; ELIZABETH CINDY LEXINTA; YOSEP PRIYONO; RATNA SUSANDARINI
Biodiversitas Journal of Biological Diversity Vol. 21 No. 8 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210804

Abstract

Abstract. Nugroho LH, Lexinta EC, Priyono Y, Susandarini R. 2020. Short Communication: Composition of terpenoid compounds in essential oils extracted from stems of eight Piper species and their role in taxonomic relationships. Biodiversitas 21: 3438-3443. Piper is widely used as source of essential oils from which many bioactive compounds with medicinal properties were used in traditional medicine. The objectives of this study were to explore the chemical composition of essential oils of eight Piper species and to identify its role in determining taxonomic relationships. Chemical composition of essential oils was analyzed using GC-MS analysis on petroleum ether extracts from stems of eight Piper species, while taxonomic relationship was determined using cluster analysis and principal component analysis. Results showed that 21 terpenoid compounds were identified as constituents of the essential oils, consisted of 4 monoterpenes, 14 sesquiterpenes, and 3 diterpenes. The composition of essential oils varied between species. Cluster analysis and principal component analysis showed that differences in the composition of essential oil compounds determined the grouping of species into two clusters. Five compounds were identified as having major role in the grouping of species. These compounds were ?-selinene, ?-caryophyllene, ?-caryophyllene, farnesyl acetone, and ?-amorphene. The results of this study offer opportunities for the use of Piper stems as source of essential oils. This study also confirms the interspecies variability in composition of essential oils, and at the same time supports the use of essential oils in chemotaxonomic studies.
Short Communication: Analysis of simple sequence repeats variations for the assessment of genotypic diversity in Centella asiatica (L.) Urb. ANSHARY MARUZY; RATNA SUSANDARINI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 2 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250233

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Abstract. Maruzy A, Susandarini R. 2024. Short Communication: Analysis of simple sequence repeats variations for the assessment of genotypic diversity in Centella asiatica (L.) Urb. Biodiversitas 25: 734-740. Centella asiatica (L.) Urb. is a perennial plant widely known as herbal ingredient in traditional and modern medicine, as well as in the cosmetics industry. This herbaceous species grows in various types of habitat, and is known for its morphological variations among populations, indicating the existence of genotypic variation. In this study, the assessment of genotypic diversity using simple sequence repeats (SSR) was carried out on C. asiatica accessions from 32 populations collected from eight mountains in Central Java. Representative individuals from four populations grow at different altitudes were sampled in each mountain. Amplification of genomic DNA using the SSR primer mCaCIR002 produced a single amplicon of 180 bp. The results of SSR analysis showed that there is high genotypic diversity in C. asiatica which is characterized by variations in the number of microsatellite repeats of (CT)10 to (CT)19. Variations in the number of repeats are mainly caused by insertions and deletions in DNA sequences at the positions 102-145. Haplotype analysis also showed high genetic diversity within and between populations of C. asiatica, indicated by the haplotype diversity of 1.00 in all populations. The haplotype network analysis indicated that there was no gene flow between the four populations at each mountain where C. asiatica populations grow. The results of this study provide evidence for the role of SSR as a suitable molecular marker for studying genotypic diversity at the intraspecific level, and confirming the morphological variability in this species.
Co-Authors ANSHARY MARUZY ELIZABETH CINDY LEXINTA Laurentius Hartanto Nugroho YOSEP PRIYONO