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All Journal Biodiversitas Journal of Biological Diversity
Mustofa Helmi Effendi
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Raya Mulyorejo, Surabaya 60115, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Presence of multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli isolated from cloacal swab of broilers in several wet markets in Surabaya, Indonesia Mustofa Helmi Effendi; WIWIEK TYASNINGSIH; YEMIMA ANGGUN YURIANTI; JOLA RAHMAHANI; NENNY HARIJANI; HANI PLUMERIASTUTI
Biodiversitas Journal of Biological Diversity Vol. 22 No. 1 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220137

Abstract

Abstract. Effendi MH, Tyasningsih W, Yurianti YA, Rahmahani J, Harijani N, Plumeriastuti H. 2021. Presence of multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli isolated from cloacal swab of broilers in several wet markets in Surabaya, Indonesia. Biodiversitas 22: 304-310.  The purpose of this research was to identify multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli from cloacal swab of broiler chicken in several wet markets in Surabaya. This study used 60 broiler chicken samples, with cloacal swab method. The samples were isolated and identified to find Escherichia coli with several procedures, MacConckey Agar (MCA), Eosin Methylene Blue Agar (EMBA), Gram staining, indole test, Methyl Red-Voges Proskauer (MR-VP), citrate, and Triple Sugar Iron Agar (TSIA). Antibiotic sensitivity test was tested by using Kirby-Bauer (Disc Diffusion Method) with antibiotics: Aztreonam, Gentamicin, Chloramphenicol, Tetracycline, and Ciprofloxacin. From research, it can be illustrated that the isolates resistant to antibiotic Ciprofloxacin were 67% and Tetracycline was 65%. Total 97% isolates were found sensitive for Aztreonam, 73% for Chloramphenicol, and 55% for Gentamicin. Twelve isolates identified for MDR and two were ESBL. It can be explained that broiler chicken from wet market should be considered as a source of transmission for MDR and ESBL of E. coli to the public health.
Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia RIBBY ANSHARIETA; SANCAKA CHASYER RAMANDINIANTO; Mustofa Helmi Effendi; HANI PLUMERIASTUTI
Biodiversitas Journal of Biological Diversity Vol. 22 No. 4 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220402

Abstract

Abstract. Ansharieta R, Ramandinianto SC, Effendi MH, Plumeriastuti H. 2021. Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia. Biodiversitas 22: 1600-1605. The emergence of extended-spectrum ?-lactamase (ESBL) producing bacteria and its increasing level has become public health issue. The presence of these bacteria in food of animal origin is quite alarming. The objective of this study was to detect and characterize Escherichia coli producing ESBL encoding genes, isolated from 200 raw cow milk samples in East Java, Indonesia. The results of this study showed that 70.5% of isolates were confirmed as E. coli, based on the morphological growth of colonies on the EMB Agar and biochemical IMViC tests. In this study, the double-disc synergy test (DDST) method was used to confirm the ESBL, and previously sorted out presumptively by using Aztreonam antibiotic disc. The antibiotics used were amoxicillin-clavulanate, ceftazidime, and cefotaxime for DDST. In addition, ESBL confirmation with Multiplex PCR method for blaCTX-M and blaTEM genes were done. The presence of ESBL-producing by E. coli isolated from raw cow’s milk in East Java were 2.12% (3/141). The PCR results showed that the double blaCTX-M and blaTEM gene harbored by 2 ESBL isolates and one blaTEM gene as many as 1 ESBL isolate. Thus, the findings of our study indicate that milk can be a good reservoir of bacteria carrying blaCTX-M and blaTEM ESBL resistance genes with the potential to affect human health.
Co-Authors HANI PLUMERIASTUTI JOLA RAHMAHANI NENNY HARIJANI RIBBY ANSHARIETA SANCAKA CHASYER RAMANDINIANTO WIWIEK TYASNINGSIH YEMIMA ANGGUN YURIANTI