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All Journal Biodiversitas Journal of Biological Diversity
WIWIEK TYASNINGSIH
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Raya Mulyorejo, Surabaya 60115, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Presence of multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli isolated from cloacal swab of broilers in several wet markets in Surabaya, Indonesia Mustofa Helmi Effendi; WIWIEK TYASNINGSIH; YEMIMA ANGGUN YURIANTI; JOLA RAHMAHANI; NENNY HARIJANI; HANI PLUMERIASTUTI
Biodiversitas Journal of Biological Diversity Vol. 22 No. 1 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220137

Abstract

Abstract. Effendi MH, Tyasningsih W, Yurianti YA, Rahmahani J, Harijani N, Plumeriastuti H. 2021. Presence of multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli isolated from cloacal swab of broilers in several wet markets in Surabaya, Indonesia. Biodiversitas 22: 304-310.  The purpose of this research was to identify multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli from cloacal swab of broiler chicken in several wet markets in Surabaya. This study used 60 broiler chicken samples, with cloacal swab method. The samples were isolated and identified to find Escherichia coli with several procedures, MacConckey Agar (MCA), Eosin Methylene Blue Agar (EMBA), Gram staining, indole test, Methyl Red-Voges Proskauer (MR-VP), citrate, and Triple Sugar Iron Agar (TSIA). Antibiotic sensitivity test was tested by using Kirby-Bauer (Disc Diffusion Method) with antibiotics: Aztreonam, Gentamicin, Chloramphenicol, Tetracycline, and Ciprofloxacin. From research, it can be illustrated that the isolates resistant to antibiotic Ciprofloxacin were 67% and Tetracycline was 65%. Total 97% isolates were found sensitive for Aztreonam, 73% for Chloramphenicol, and 55% for Gentamicin. Twelve isolates identified for MDR and two were ESBL. It can be explained that broiler chicken from wet market should be considered as a source of transmission for MDR and ESBL of E. coli to the public health.
Molecular detection of iron gene on multidrug resistant avian fecal Escherichia coli isolated from broiler on traditional markets, Surabaya, Indonesia MARIANA FEBRILIANTI RESILINDA PUTRI; IRFAN ALIAS KENDEK; FRESHINTA JELLIA WIBISONO; MUSTOFA HELMI EFFENDI; DADIK RAHARDJO; WIWIEK TYASNINGSIH; EMMANUEL NNABUIKE UGBO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 12 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241207

Abstract

Abstract. Putri MFR, Kendek IA, Wibisono FJ, Effendi MH, Rahardjo D, Tyasningsih W, Ugbo EN. 2023. Molecular detection of iron gene on multidrug resistant avian fecal Escherichia coli isolated from broiler on traditional markets, Surabaya, Indonesia. Biodiversitas 24: 6454-6460. Avian Fecal Escherichia coli (AFEC) is the cause of colibacillosis, which often infects the poultry industry throughout the world. The virulence gene influences the iroN factor, which causes systemic infections in poultry. This research aimed to determine Multidrug Resistance (MDR) in AFEC obtained from traditional markets in Surabaya. There were 96 cloacal swab samples from broiler chickens. The samples were isolated and identified using culture media in the form of Eosin Methylene Blue Agar (EMBA), gram stain, Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), Simmons Citrate Agar (SCA), Voges Proskauer (MR-VP) indole and methyl test; detection of multidrug resistance using Mueller-Hinton Agar (MHA) media. The results of the isolation and identification of Escherichia coli bacteria in this study showed that 60.4% of the isolates were positive for Escherichia coli. Escherichia coli is resistant to tetracycline antibiotics by 56%, ciprofloxacin by 55%, antibiotics aztreonam 29%, kanamycin 20%, and chloramphenicol 18%. The multidrug resistance test result on Escherichia coli was 25.8%. The PCR test results for the iroN gene were 40%. Therefore, there are MDR and iroN genes in avian fecal Escherichia coli in Surabaya traditional markets; APEC with iroN gene poses the potential to affect public health.
Molecular detection of hlyF gene on multidrug resistance of avian pathogenic Escherichia coli isolated from ducks on wet markets of Surabaya, Indonesia IRFAN ALIAS KENDEK; MARIANA FEBRILIANTI RESILINDA PUTRI; FRESHINTA JELLIA WIBISONO; MUSTOFA HELMI EFFENDI; WIWIEK TYASNINGSIH; EMMANUEL NNABUIKE UGBO; NNABUIFE BERNARD AGUMAH
Biodiversitas Journal of Biological Diversity Vol. 25 No. 3 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250341

Abstract

Abstract. Kendek IA, Putri MFR, Wibisono FJ, Effendi MH, Tyasningsih W, Ugbo EN, Agumah NB. 2024. Molecular detection of hlyF gene on multidrug resistance of avian pathogenic Escherichia coli isolated from ducks on wet markets of Surabaya, Indonesia. Biodiversitas 25: 1246-1252. Avian Pathogenic Escherichia coli (APEC) is a strain of pathogen that can cause colibacillosis in poultry, including ducks worldwide. This disease can be influenced by various virulence genes, one of which is the hlyF gene which can cause systemic disorders and potentially become zoonotic in poultry. This study aimed to detect the hlyF virulence gene from the multidrug resistance (MDR) properties of Escherichia coli originating from 158 duck cloacal swab samples from seven live markets in Surabaya. Samples were isolated and identified using Eosin Methylene Blue Agar (EMBA) and MacConkey Agar (MCA) media, Gram staining, and then continued with biochemical tests of Triple Sugar Iron Agar (TSIA), Simmons Citrate Agar (SCA), Sulfide Indole Motility (SIM), Methyl red (MR) and Voges-Proskauer (VP). Samples were tested for MDR properties and continued with confirmation of the hlyF virulence gene using PCR. Based on the results of this study, it showed that 85% (134/158) were positive for Escherichia coli. Escherichia coli bacteria experienced resistance to the antibiotic erythromycin 96% (129/134), ciprofloxacin 16% (22/134), antibiotics gentamicin 15% (20/134), aztreonam 4.4% (6/134) and chloramphenicol 3% (5/58). The MDR test results in this study were 15% (20/134), while the PCR test results for the hlyF gene were 60% (12/20). Therefore, Escherichia coli was found to have the hlyF virulence gene, an MDR in Escherichia coli bacteria at the Surabaya live market. Furthermore, APEC strains with the hlyF gene, an MDR, potentially affect public health.
Co-Authors DADIK RAHARDJO EMMANUEL NNABUIKE UGBO EMMANUEL NNABUIKE UGBO FRESHINTA JELLIA WIBISONO FRESHINTA JELLIA WIBISONO HANI PLUMERIASTUTI IRFAN ALIAS KENDEK JOLA RAHMAHANI MARIANA FEBRILIANTI RESILINDA PUTRI MUSTOFA HELMI EFFENDI Mustofa Helmi Effendi NENNY HARIJANI NNABUIFE BERNARD AGUMAH YEMIMA ANGGUN YURIANTI