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All Journal Biodiversitas Journal of Biological Diversity
Shuichi Shimma
Department of Biotechnology, Graduate School of Engineering, Osaka University. 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI Jujun Ratnasari; Rizkita Rachmi Esyanti; Marselina Irasonia Tan; Lia Dewi Juliawaty; Shuichi Shimma
Biodiversitas Journal of Biological Diversity Vol. 22 No. 3 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220312

Abstract

Abstract. Ratnasari J, Esyanti RR, Tan MI, Juliawaty LD, Shimma S. 2021. Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI. Biodiversitas 22: 1172-1178. Cryptobrachytone C is an active compound isolated from leaves of Cryptocarya pulchrinervia, an indigenous plant from Indonesia. Cryptobrachytone C is cytotoxic against P388 leukemia cancer cell lines. A profile of cryptobrachytone C accumulation in leaves based on physiological age is necessary to study cryptobrachytone C production in the plant since it has anticancer potential. In situ profiling of cryptobrachytone C accumulation in leaves was carried out by imaging techniques using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). The cryptobrachytone C ionization efficiency was carried out by derivatization using Girard-T reagent and coating with ?-cyano-4-hydroxycinnamic acid (?-CHCA) matrix. Scanned leaves show higher accumulation intensity in the second leaf. Quantitative accumulation profiling was conducted using GC-MS, where the highest amount of cryptobrachytone C was on the second leaf at 87.07 ± 47.21 mg. This showed that the most effective isolation of cryptobrachytone C would be obtained from young leaves of Cryptocarya pulchrinervia. This profile would play a role in cryptobrachytone C production in-situ or ex-situ using tissue culture.  
Co-Authors Jujun Ratnasari Lia Dewi Juliawaty Marselina Irasonia Tan Rizkita Rachmi Esyanti