Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI

Jujun Ratnasari (Plant Sciences and Biotechnology Laboratory, School of Life Sciences and Technology, Institut Teknologi Bandung. Jl. Ganesa 10, Bandung 40132, West Java, Indonesia)
Rizkita Rachmi Esyanti (Plant Sciences and Biotechnology Laboratory, School of Life Sciences and Technology, Institut Teknologi Bandung. Jl. Ganesa 10, Bandung 40132, West Java, Indonesia)
Marselina Irasonia Tan (Animal Physiology, Developmental Biology and Biomedical Sciences Laboratory, School of Life Sciences and Technology, Institut Teknologi Bandung. Jl. Ganesa 10, Bandung 40132, West Java, Indonesia)
Lia Dewi Juliawaty (Organic Chemistry Laboratory, Chemistry Program, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung. Jl. Ganesa 10, Bandung 40132, West Java, Indonesia)
Shuichi Shimma (Department of Biotechnology, Graduate School of Engineering, Osaka University. 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan)

Biodiversitas Journal of Biological Diversity
Vol. 22 No. 3 (2021)

Abstract. Ratnasari J, Esyanti RR, Tan MI, Juliawaty LD, Shimma S. 2021. Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI. Biodiversitas 22: 1172-1178. Cryptobrachytone C is an active compound isolated from leaves of Cryptocarya pulchrinervia, an indigenous plant from Indonesia. Cryptobrachytone C is cytotoxic against P388 leukemia cancer cell lines. A profile of cryptobrachytone C accumulation in leaves based on physiological age is necessary to study cryptobrachytone C production in the plant since it has anticancer potential. In situ profiling of cryptobrachytone C accumulation in leaves was carried out by imaging techniques using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). The cryptobrachytone C ionization efficiency was carried out by derivatization using Girard-T reagent and coating with ?-cyano-4-hydroxycinnamic acid (?-CHCA) matrix. Scanned leaves show higher accumulation intensity in the second leaf. Quantitative accumulation profiling was conducted using GC-MS, where the highest amount of cryptobrachytone C was on the second leaf at 87.07 ± 47.21 mg. This showed that the most effective isolation of cryptobrachytone C would be obtained from young leaves of Cryptocarya pulchrinervia. This profile would play a role in cryptobrachytone C production in-situ or ex-situ using tissue culture.