Article Per Year (5 Year)
p-Index From 2021 - 2026
1.325
P-Index
This Author published in this journals
All Journal Mitra Mahajana: Jurnal Pengabdian Masyarakat Biodiversitas Journal of Biological Diversity
AGNES VIRGINIA SIMAMORA
Universitas Nusa Cendana, Kupang City, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Industrial & Manufacturing Engineering Public Health Social Sciences

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search

 1 
Endophytic fungi as potential biocontrol agents of Phytophthora palmivora in the cocoa plant AGNES VIRGINIA SIMAMORA; MAYAVIRA VERONICA HAHULY; JULINDA BD Henuk
Biodiversitas Journal of Biological Diversity Vol. 22 No. 5 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220519

Abstract

Abstract. Simamora AV, Hahuly MV, Henuk JBD. 2021. Endophytic fungi as potential biocontrol agents of Phytophthora palmivora in the cocoa plant. Biodiversitas 22: 2601-2609. In Indonesia, the cocoa tree is one of the essential cultivation crops for farmers. Despite the importance of cocoa cultivation in Indonesia's economy, the productivity of this crop has declined. Cocoa black pod disease caused by Phytophthora palmivora (Butl.) is one of the most severe diseases affecting this crop worldwide, with average annual losses above 40%. Instead of using manufactured chemicals, biological control is an effective and eco-friendly alternative control measure against plant pathogens. This work aimed to assess the potential of endophytic fungi isolated from healthy cocoa pods to control Phytophthora palmivora in vitro and in vivo. Endophytic fungal isolates were classified based on the morphological characteristics of their cultures and reproductive structures. All isolates found were tested to inhibit P. palmivora in the dual culture method, and the best 10 isolates were continued for detached pod assay. Then, the best five isolates (Aspergillus4, Aspergillus5, Aspergillus6, Fusarium6, Ramichloridium sp.) were evaluated for their capability to reduce P. palmivora in cocoa seedlings and in the field plants. Aspergillus, Fusarium, and Ramichloridium showed maximum activity against P. palmivora in dual culture, pod, and seedling assays. Nevertheless, when all these five isolates were applied in the field, they did not suppress the disease development.
In vitro screening of fungal endophytes from sandalwood (Santalum album) as antagonists to phytopathogens AGNES V. SIMAMORA; MAYAVIRA V. HAHULY; PETRONELLA S. NENOTEK; YENNY RAJA KANA; M. KASIM; RODDIALEK POLLO; ANTONIUS R. B. OLA; FADLAN PRAMATANA
Biodiversitas Journal of Biological Diversity Vol. 25 No. 1 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250142

Abstract

Abstract. Simamora AV, Hahuly MV, Nenotek PS, Kana YR, Kasim M, Pollo R, Ola AR, Pramatana F. 2024. In vitro screening of fungal endophytes from sandalwood (Santalum album) as antagonists to phytopathogens. Biodiversitas 25: 361-371. Endophytic fungi possess bioactive compounds and generate secondary metabolites like their host plants. The phytochemical compounds found in sandalwood plants (Santalum album L.) are recognized for their anti-pathogenic properties, suggesting the potential of sandalwood endophytic fungi as effective anti-phytopathogens. Alternaria solani and Fusarium oxysporum threaten tomato plants, whereas Phytophthora palmivora is particularly detrimental to cocoa plants. The aims of this study were: (i) to obtain isolates of endophytic fungi from roots, stems, and leaves of sandalwood plants and (ii) to assess the ability of endophytic fungi as anti-phytopathogens in vitro. Endophytic fungi were isolated through a direct plating method and next by purification and identification. All obtained endophytic fungi underwent testing for their antagonistic potential against A. solani, F. oxysporum, and P. palmivora in vitro through the dual culture method. The inhibition percentage was analyzed using variance analysis and further examined with the 5% HSD test. The study successfully isolated 104 endophytic fungal isolates from the sandalwood; 29 isolates were from the roots, 33 from the stems, and 42 from the leaves. All endophytic isolates demonstrated the ability to inhibit the growth of A. solani, F. oxysporum, and P. palmivora in vitro, with various inhibition percentages ranging from 26.7 to 83.3%. Further research will concentrate on secondary metabolites from selected isolates showing inhibition levels exceeding 70%.
Insect vectors of banana blood disease in Flores Island, East Nusa Tenggara, Indonesia MAYAVIRA V. HAHULY; I WAYAN MUDITA; AGNES V. SIMAMORA; PETRONELLA S. NENOTEK; SRI WIDINUGRAHENI; VIKTORIANUS JEHAUT; FADLAN PRAMATANA
Biodiversitas Journal of Biological Diversity Vol. 26 No. 8 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260812

Abstract

Abstract. Hahuly MV, Mudita IW, Simamora AV, Nenotek PS, Widinugraheni S, Jehaut V, Pramatana F. 2025. Insect vectors of banana blood disease in Flores Island, East Nusa Tenggara, Indonesia. Biodiversitas 26: 3791-3802. Banana Blood Disease (BBD) poses a significant threat to banana cultivation in Indonesia. Following severe outbreaks on Sumba Island, the disease spread to the neighboring Flores Island in mid-2022, with a particularly pronounced impact on the predominant cultivar, Musa sp. ABB ‘Kepok plantain.’ Despite prior outbreaks, awareness and understanding of the disease and its insect vectors remain limited among agricultural officers, village leaders, and farmers on Flores Island. The pathogen, Ralstonia syzygii subsp. celebesensis (Rsc), is known to be transmitted by insect vectors; however, no prior data existed regarding vectors on Flores Island. This knowledge gap has impeded effective disease management and allowed the disease to spread unnoticed. This study aimed to assess the spread and incidence of BBD and investigate potential insect vectors involved in its transmission. A survey conducted across five districts in western Flores confirmed the presence of BBD in all surveyed areas, with varying incidence rates among banana cultivars. The highest incidence (65.88%) was recorded in Musa sp. ABB ‘Kepok plantain,’ followed by Musa sp. AAB ‘Raja Sereh banana,’ Musa sp. AAA ‘Barangan banana,’ Musa sp. AAA ‘Cavendish banana,’ and Musa sp. BB ‘Klutuk plantain.’ Insect surveys identified eight species visiting male banana inflorescences. Among them, Oscinella sp. (Diptera: Chloropidae) and Bactrocera sp. (Diptera: Tephritidae) were the most widespread and abundant. These species have previously been reported as BBD vectors in other regions. They were likely key contributors to the spread of Rsc on Flores Island.
In vitro growth assessment of Trichoderma spp. from paddy rhizosphere on glyphosate- and fipronil- containing medium AGNES V. SIMAMORA; PETRONELLA S. NENOTEK; MAYAVIRA V. HAHULY; LILY F. ISHAQ; SRI WIDINUGRAHENI; YENNY RAJA KANA; SPERATUS C. U. PASI; ANTONIUS R. B. OLA; EVERT Y. HOSANG; BAYU R. FITRIADI
Biodiversitas Journal of Biological Diversity Vol. 26 No. 5 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260502

Abstract

Abstract. Simamora AV, Nenotek PS, Hahuly MV, Ishaq LF, Widinugraheni S, Kana YR, Pasi SCU, Ola ARB, Hosang EY, Fitriadi BR. 2025. In vitro growth assessment of Trichoderma spp. from paddy rhizosphere on glyphosate- and fipronil- containing medium. Biodiversitas 26: 2028-2038. Agricultural practices worldwide increasingly rely on synthetic pesticides to enhance crop productivity and manage pest infestations. Glyphosate, a widely used herbicide, and fipronil, a broad-spectrum insecticide, are extensively applied due to their effectiveness. However, prolonged use of these agrochemicals raises significant environmental concerns, particularly soil contamination, which disrupts microbial diversity and degrades soil health. The persistence of glyphosate and fipronil in soil ecosystems challenges sustainable agriculture, necessitating the exploration of bioremediation strategies. Research on the ability of Trichoderma spp. to degrade these pesticides remains limited, highlighting the need for this study. This research aimed to (i) isolate Trichoderma spp. from paddy fields with frequent glyphosate and fipronil application; (ii) assess their growth on PDA media supplemented with glyphosate and fipronil; and (iii) evaluate their in vitro potential for pesticide degradation. Growth assessments were conducted on PDA media supplemented with glyphosate at 0 mL/L (control), 5.0 mL/L (recommended concentration), and 12.5 mL/L, and with fipronil at 0 mL/L (control), 2.0 mL/L (recommended concentration), and 4.0 mL/L. The degradation potential was evaluated using a modified dual-culture method with Fusarium oxysporum as the indicator pathogen. The results showed four Trichoderma isolates were obtained, namely T. harzianum 01, T. camerunense 02, T. harzianum 03, and Trichoderma 04. Growth analysis indicated that T. harzianum 01 had the highest colony diameter (9.0 cm) and exhibited complete resistance at recommended glyphosate and fipronil concentrations, while T. camerunense 02 was the most sensitive. Relative inhibition rate analysis confirmed that glyphosate had a stronger inhibitory effect than fipronil. Antagonism tests revealed that all isolates suppressed Fusarium oxysporum, with inhibition rates ranging from 70.59 to 83.96%, indicating strong biocontrol potential. These findings demonstrate the resilience of Trichoderma spp. under pesticide exposure and their capacity to inhibit phytopathogens, supporting their potential as bioremediation and biological control agents for sustainable soil management.
Phytochemical screening, antimicrobial and antidiabetic activity of n-hexane extract of Macaranga depressa leaves KHOLIFATU ROSYIDAH; RAHMAT EKO SANJAYA; UTAMI IRAWATI; NAWWAL HIKMAH; RIZKY AULIA FITRIANI; JANNESA TRI ROSADI; IRAWAN WIJAYA KUSUMA; YADI YADI; AGNES V. SIMAMORA; ANTONIUS R. B. OLA; I GUSTI MADE NGURAH BUDIANA
Biodiversitas Journal of Biological Diversity Vol. 26 No. 10 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261030

Abstract

Abstract. Rosyidah K, Sanjaya RE, Irawati U, Hikmah N, Fitriani RA, Rosadi JT, Kusuma IW, Yadi, Simamora AV, Ola ARB, Budiana IGMN. 2025. Phytochemical screening, antimicrobial and antidiabetic activity of n-hexane extract of Macaranga depressa leaves. Biodiversitas 26: 5156-5168. The Macaranga genus has been used in folk medicine to treat pathological disorders like diabetes. However, the chemical constituents and bioactivity of Macaranga depressa have never been studied. Thus, this study combined phytochemical profiling, antimicrobial evaluation, and in silico molecular docking of M. depressa. It aims to determine the phytochemical profile, antimicrobial, and antidiabetic activities of n-hexane extract of M. depressa leaves. Sample extraction was carried out by maceration method. The phytochemical profile of the plant was determined by phytochemical screening method and analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) method. Antimicrobial activity was tested in vitro using disc diffusion method. Antidiabetic activity was tested in vitro and in silico including drug similarity analysis, ADME (Absorption, Distribution, Metabolism, and Excretion) pharmacokinetic profile, toxicity potential, and molecular docking interaction. Phytochemical screening revealed the presence of alkaloids, flavonoids, terpenoids, and tannins. GC-MS analysis identified stigmast-4-en-3-one (32.76%) and 4',6'-dimethoxy-2'-hydroxy-3-nitrochalcone (14.82%) as the major components. The extract exhibited significant antimicrobial activity against Candida albicans (18.0±0.44 mm), Escherichia coli (19.4±0.57 mm), Pseudomonas aeruginosa (23.5±1.32 mm), and Staphylococcus aureus (21.2±0.47 mm). The in vitro antidiabetic test using the ?-glucosidase enzyme showed that the n-hexane extract of M. depressa had an IC50 value of 17.561±0.471 ppm. ADMET analysis indicated that the major components followed Lipinski’s rule, exhibited drug-like properties, and were non-mutagenic. Molecular docking results showed strong interactions between the major compounds and maltase-glucoamylase (2QMJ) and PPAR-? (2Q5S). These findings suggest that the n-hexane extract of M. depressa leaves has promising antimicrobial and antidiabetic potential.
Morphometric diversity of wild poisonous arbila (Phaseolus lunatus) germplasm from Timor and Sumba Islands, Indonesia AGNES V. SIMAMORA; LINCE MUKKUN; MAYAVIRA V. HAHULY; PETRONELLA SAHYANTI NENOTEK; YOSEP SERAN MAU; NORMAN RIWU KAHO; YOSEPH NAHAK SERAN; ENOS TANGKE ARUNG; EVERT Y. HOSANG
Biodiversitas Journal of Biological Diversity Vol. 27 No. 5 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270511

Abstract

Abstract. Simamora AV, Mukkun L, Hahuly MV, Nenotek PS, Mau YS, Kaho NR, Seran YN, Arung ET, Hosang EY. 2026. Morphometric diversity of wild poisonous arbila (Phaseolus lunatus) germplasm from Timor and Sumba Islands, Indonesia. Biodiversitas 27 (5): d270511. https://doi.org/10.13057/biodiv/d270511. Poisonous arbila (Phaseolus lunatus) is an underutilized legume traditionally consumed after detoxification by dryland communities in East Nusa Tenggara, Indonesia. Despite its importance as an emergency food resource, information on its phenotypic variation remains limited. This study aimed to characterize seed morphometric variation in wild and semi-managed arbila germplasm collected from six locations across Timor and Sumba Islands. A total of 108 accessions were evaluated using twelve seed traits, including eight qualitative characters (seed shape, seed coat color and pattern, hilum shape, testa texture, and eye color) and four quantitative characters (seed length, width, thickness, and 100-seed weight). Qualitative traits showed high polymorphism, particularly in seed coat pigmentation and patterning, while quantitative traits exhibited wide ranges (seed length: 10.33-23.70 mm; 100-seed weight: 25.40-56.50 g). Multivariate analyses (principal component analysis and hierarchical clustering) indicated that the first four principal components explained 91.6% of the total variance, with seed size–related traits dominating the primary axis (PC1 = 65.2%). Clustering results identified one major group comprising most accessions and a small number of divergent accessions, consistent with a continuous phenotypic gradient rather than discrete group separation. These results highlight considerable morphometric diversity in arbila and indicate that phenotypic differentiation is primarily structured along this gradient. The findings provide a useful basis for germplasm conservation and highlight the potential of arbila for selection, breeding, and future studies on domestication and genetic diversity.
UTILIZATION OF TRICHO-COMPOST AND LOCAL MICROORGANISMS TO SUPPORT ORGANIC FARMING FOR THE ORA ET LABORA FARMER GROUP IN KUPANG CITY Mayavira Veronica Hahuly; Agnes Virginia Simamora; Petronela Syahyanti Nenotek; Agustina Etin Nahas; Shirly Seahan Oematan; Effy Roefaida; Ewaldus Tan; Jericho Albert Balol; Theodosius Lau Base; Fadlan Pramatana
Mitra Mahajana: Jurnal Pengabdian Masyarakat Vol. 7 No. 2 (2026): Volume 7 Nomor 2 Tahun 2026
Publisher : LPPM Universitas Flores

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/mahajana.v7i2.8456

Abstract

The “Ora et Labora” Farmer Group in Naioni Village, Kupang City, has developed horticultural and livestock farming on a three-hectare area cultivating long beans, tomatoes, and chilli peppers using a drip irrigation system. Despite these advancements, farmers remain dependent on chemical fertilizers and pesticides, which over time damage soil structure, reduce fertility, and disrupt the balance of soil microorganisms. To address this issue, an organic farming system was introduced as an environmentally friendly alternative using tricho-compost based on Trichoderma spp. and Local Microorganisms (MOL). Tricho-compost improves nutrient availability and suppresses soil-borne pathogens, while MOL acts as a natural starter for organic matter fermentation and a potential biocontrol agent. This Community Service Program (PKM) aimed to enhance farmers’ understanding and skills in producing and applying tricho-compost and MOL. The results demonstrated improved knowledge, technical ability, and motivation among farmers to independently implement organic farming, thereby promoting sustainable and environmentally friendly cultivation systems.
Co-Authors Agustina Etin Nahas ANTONIUS R. B. OLA ANTONIUS R. B. OLA ANTONIUS R. B. OLA BAYU R. FITRIADI Effy Roefaida ENOS TANGKE ARUNG EVERT Y. HOSANG EVERT Y. HOSANG Ewaldus Tan I GUSTI MADE NGURAH BUDIANA I WAYAN MUDITA IRAWAN WIJAYA KUSUMA JANNESA TRI ROSADI Jericho Albert Balol JULINDA BD Henuk KHOLIFATU ROSYIDAH LILY F. ISHAQ LINCE MUKKUN M. KASIM MAYAVIRA V. HAHULY MAYAVIRA V. HAHULY MAYAVIRA V. HAHULY Mayavira Veronica Hahuly MAYAVIRA VERONICA HAHULY NAWWAL HIKMAH NORMAN RIWU KAHO Petronela Syahyanti Nenotek PETRONELLA S. NENOTEK PETRONELLA S. NENOTEK PETRONELLA SAHYANTI NENOTEK Pramatana, Fadlan RAHMAT EKO SANJAYA RIZKY AULIA FITRIANI RODDIALEK POLLO Shirly Seahan Oematan SPERATUS C. U. PASI SRI WIDINUGRAHENI Theodosius Lau Base UTAMI IRAWATI VIKTORIANUS JEHAUT YADI YADI YENNY RAJA KANA YENNY RAJA KANA YOSEP SERAN MAU YOSEPH NAHAK SERAN