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All Journal Biodiversitas Journal of Biological Diversity
R. SUSANTI
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang. Gedung D6 Lantai 1, Kampus Sekaran, Gunungpati, Semarang 50229, Central Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Effects of production system on the gut microbiota diversity and IgA distribution of Kampong chickens, Indonesia R. SUSANTI; WULAN CHRISTIJANTI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 2 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230252

Abstract

Abstract. Susanti R, Christijanti W. 2022. Effects of production system on the gut microbiota diversity and IgA distribution of Kampong chickens. Biodiversitas 23: 1082-1090. Native chickens (Kampong chickens) are poultry in Indonesia that is raised using a traditional production system. This study aimed to analyze the differences of the Kampong chicken production system (extensive/E and semi-intensive/SI) on the diversity and abundance of gut microbiota and the distribution of IgA. Each sample of Kampong chickens was slaughtered, then their thoracic and abdominal cavities were dissected, and their intestines were collected. Furthermore, the intestinal tissue was processed to make histological preparations for immunohistochemical analysis of IgA. Gut contents of 25 g were taken aseptically and used for next-generation sequencing (NGS) and GC-MS analysis. The results showed 12 bacterial phyla in the intestines of SI and E chicken. E chickens had a higher abundance and diversity of microbiota than the SI chickens. The phylum Firmicutes dominated the E and SI chickens' gut microbiota (>50%). SI chickens have a higher Firmicutes/Bacteroidetes ratio than the E chickens. The SIgA distribution showed an IRS score of 4 (moderate), both in E and SI chickens. It was concluded that the production system affects the intestinal microbiota's abundance and diversity but not on the intestine IgA of Kampong Chickens. This study highlights that based on the Firmicutes/Bacteroidetes ratio, the semi-intensive system is more suitable for Kampong chicken meat and eggs than the extensive system.
Genetic diversity of Central Javanese duck (Indonesia) based on Inter Simple Sequence Repeat markers R. SUSANTI; AGUSTIN DIAN KARTIKASARI; FITRI ARUM SASI; DYKEN DWI ARLINDA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 4 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230412

Abstract

Abstract. Susanti R, Kartikasari AD, Sasi FA, Arlinda DD. 2022. Genetic diversity of Central Javanese duck (Indonesia) based on Inter Simple Sequence Repeat markers. Biodiversitas 23: 1807-1813. Genetic resources of local livestock need to be documented and conserved to prevent genetic erosion of the germplasm. This study aimed to determine the genetic diversity of domestic ducks (Anas platyrhynchos domesticus) in Central Java, Indonesia, based on Inter Simple Sequence Repeat (ISSR) markers. There were seven types of Central Javanese ducks used in this research. DNA was extracted from feathers samples. To give rise to ISSR profile of duck DNA, 5 different ISSR markers were used. Annealing temperature used in PCR was the result of the optimization. The ISSR-PCR results showed that all primers (100%) were polymorphic. From a total of five ISSR primers, resulted in 44 obvious bands in all duck samples, with 41 (91.78%) bands being polymorphic. The quantity of alleles was numerous from 7-14 polymorphic loci, the mean quantity of alleles according to locus was 8.8, and the average PIC score for the Central Javanese duck was 0.777. The relationship investigation showed that the Central Javanese local duck was clustered into two primary offshoots, one heading to Pengging duck breeds and another one consisting of all other Central Javanese local duck. The genetic diversity among Central Javanese local duck based on ISSR primer was high. The ISSR loci were polymorphic for Central Javanese duck and may be useful for study of genetic diversity and genome evolution of Central Javanese ducks in the future.
Characterization of microbiota and secretory Ig-A in the domestic duck (Anas platyrhynchos) small and large intestine R. SUSANTI; NUR RAHAYU UTAMI; ARI YUNIASTUTI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 4 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240461

Abstract

Abstract. Susanti R, Utami NR, Yuniastuti A. 2023. Characterization of microbiota and secretory Ig-A in the domestic duck (Anas platyrhynchos)small and large intestine. Biodiversitas 24: 2458-2466. The Microbiome composition could affect the duck's intestines' microenvironment, which may shape the microstructure's anatomy, metabolism, and immune system. Therefore, this study aims to characterize the intestinal microbiome's abundance, diversity, and IgA distribution in ducks' intestines. This study took 15 healthy domestic ducks (Anas platyrhynchosLinnaeus, 1758) from an intensive duck farm in Central Java, Indonesia. Five grams of each small and large intestine content were collected aseptically for metagenomic analysis. Then, the intestine organs were taken for immunohistochemistry preparations to depict the IgA distribution. The results showed that the small intestine has a greater bacterial abundance community, with 18 phyla, while only 13 are found in the large intestine. Interestingly, three phyla: Firmicutes, Actinobacteria, and Bacteroidetes, were found dominantly in both organs. However, the comparison of Firmicutes and Bacteroidetes ratio was higher in the small intestine (2357.76) than large intestine (10.64). The IgA distribution in the small and large intestines showed intermediate staining intensity (score: 2.07-2.20) and a final Allred score of 5.0 (positive). Even though dysbiosis microbiome was present in intestines with dominant species Streptococcus and Enterococcus, it seems no significant IgA over secretion. Understanding duck's gut immune response is important because it is highly tolerant to the pathogens that make them play an important role as an environmental reservoir for pathogenic viruses and bacteria and may potentially comprehend future infectious disease outbreaks.
Co-Authors AGUSTIN DIAN KARTIKASARI ARI YUNIASTUTI DYKEN DWI ARLINDA FITRI ARUM SASI NUR RAHAYU UTAMI WULAN CHRISTIJANTI