SANTIKA SARI
Plant Breeding Laboratory, Faculty of Agriculture, Universitas Padjadjaran. Jl. Raya Bandung Sumedang Km. 21, Sumedang 45363, West Java, Indonesia

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Evaluation of agronomic and fruit quality traits of miraculin transgenic tomato: Agronomic and fruit quality traits of miraculin transgenic tomato NONO CARSONO; NIA DESIANA; FERI M. NURRIZQI; IQBAL F. ELFAKHRIANO; ANAS ANAS; SANTIKA SARI; KUSUMIYATI KUSUMIYATI; RYO OHSAWA; AYAKO SHIMONO; HIROSHI EZURA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 4 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230435

Abstract

Abstract. Carsono N, Desiana N, Nurrizqi FM, Elfakhriano IF, Anas, Sari S, Kusumiyati, Ohsawa R, Shimono A, Ezura H. 2022. Evaluation of agronomic and fruit quality traits of miraculin transgenic tomato. Biodiversitas 23: 2004-2009. Tomato cv. Moneymaker has been inserted with miraculin gene which can modify sour taste becomes sweet in the human tongue and, thus, it has potential as a sweetener. Evaluation of agronomic and fruit quality traits for this special transgenic tomato is a major concern that should be performed as substantial equivalence. The study's objective was to compare agronomic and fruit quality traits of transgenic miraculin tomato with those of its wild-type. The experiment employed a randomized block design, one genotype factor with two levels (transgenic vs. non-transgenic tomato cv. Moneymaker), and each was four replicates. Data were subjected to the Student t-test. The study revealed no significant differences between the transgenic tomato plants cv. Moneymaker and the wild type for important agronomic traits such as plant height, stem diameter, leaf area index, fruit weight, fruit weight per cluster, fruit weight per plant, fruit diameter, number of fruits per cluster, number of fruits per plant, and harvest index of miraculin. Some fruit quality traits such as fruit shelf-life, fruit hardness, total soluble solids, and content of glucose, sucrose, and fructose were equivalent to those of its counterpart, suggesting that miraculin transgene did not affect the expression of the observed traits. The miraculin transgenic tomato traits were more likely identical to those of wild-type except for the existence of the miraculin transgene. This will open the possibility for this special transgenic tomato to undergo further environmental safety assessment in the field test facility as a part of the biosafety assessment of genetically modified organism (GMO).
Agronomic characteristics and genetic relationship of putative transgenic rice lines of cv. Fatmawati with the Glu-1Dx5 transgene Nono Carsono; GIGIH IBNU PRAYOGA; SANTIKA SARI; MEDDY RACHMADI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 1 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230135

Abstract

Abstract. Carsono N, Prayoga GI, Sari S, Rachmadi M. 2021. Agronomic characteristics and genetic relationship of putative transgenic rice lines of cv. Fatmawati with the Glu-1Dx5 transgene. Biodiversitas 23: 291-298. Transgenic rice lines cv. Fatmawati with the Glu-1Dx5 transgene (encoding high molecular weight glutenin sub-unit Dx5 from bread wheat) has been produced in order to improve dough functionality of rice flour. These lines have reached T1 (transformant-1) dan T2 (transformant-2) generations. Some transgenic rice plants obtained from in vitro culture and transformation events frequently show phenotypic and genotypic variations from the original plants, thus evaluation of agronomic traits of these transgenic rice is highly important in order to obtain rice genotypes with the best agronomic traits that will be integrated into rice breeding program. Nine transgenic rice lines and two check lines, i.e. seed-derived and callus-derived rice lines, were used in this experiment. A comparison between transgenic rice traits with those of non-transgenic checks was done by student’s t-test and relationship among transgenic rice lines was evaluated by UPGMA (Unweighted Pair Group Method with Mean Arithmetic) method using NT Sys (Numerical Taxonomy and Multivariate Analysis System). Results indicated that agronomic traits of transgenic rice lines were similar to those of non-transgenic check except for number of productive tillers (T2-11, T2-12, T2-20), the maximum number of tillers (T2-7, T2-11, T2-12, T2-20), number of filled grains (T1-45) and days to maturity (all T2 lines). It suggests that somaclonal variations, gene insertional effect, genetic and epigenetic mutations might occur. Genetic relationship between putative transgenic and non-transgenic checks was closely related, although somaclonal variants were found. The selected transgenic rice lines will be incorporated into rice breeding programs.