Biodiversitas Journal of Biological Diversity
Vol. 23 No. 4 (2022)

Evaluation of agronomic and fruit quality traits of miraculin transgenic tomato: Agronomic and fruit quality traits of miraculin transgenic tomato

NONO CARSONO (Plant Breeding Laboratory, Faculty of Agriculture, Universitas Padjadjaran. Jl. Raya Bandung Sumedang Km. 21, Sumedang 45363, West Java, Indonesia)
NIA DESIANA (Agrotechnology Study Program, Faculty of Agriculture, Universitas Padjadjaran. Jl. Raya Bandung Sumedang Km. 21, Sumedang 45363, West Java, Indonesia)
FERI M. NURRIZQI (Agrotechnology Study Program, Faculty of Agriculture, Universitas Padjadjaran. Jl. Raya Bandung Sumedang Km. 21, Sumedang 45363, West Java, Indonesia)
IQBAL F. ELFAKHRIANO (Agrotechnology Study Program, Faculty of Agriculture, Universitas Padjadjaran. Jl. Raya Bandung Sumedang Km. 21, Sumedang 45363, West Java, Indonesia)
ANAS ANAS (Plant Breeding Laboratory, Faculty of Agriculture, Universitas Padjadjaran. Jl. Raya Bandung Sumedang Km. 21, Sumedang 45363, West Java, Indonesia)
SANTIKA SARI (Plant Breeding Laboratory, Faculty of Agriculture, Universitas Padjadjaran. Jl. Raya Bandung Sumedang Km. 21, Sumedang 45363, West Java, Indonesia)
KUSUMIYATI KUSUMIYATI (Plant Breeding Laboratory, Faculty of Agriculture, Universitas Padjadjaran. Jl. Raya Bandung Sumedang Km. 21, Sumedang 45363, West Java, Indonesia)
RYO OHSAWA (Tsukuba Plant Innovation Research Center, University of Tsukuba. 2-Amakubo, Tsukuba, Ibaraki 305-0005, Japan)
AYAKO SHIMONO (Department of Biology, Faculty of Science, Toho University. Funabashi, Chiba, 274-8510, Japan)
HIROSHI EZURA (Tsukuba Plant Innovation Research Center, University of Tsukuba. 2-Amakubo, Tsukuba, Ibaraki 305-0005, Japan)



Article Info

Publish Date
10 Apr 2022

Abstract

Abstract. Carsono N, Desiana N, Nurrizqi FM, Elfakhriano IF, Anas, Sari S, Kusumiyati, Ohsawa R, Shimono A, Ezura H. 2022. Evaluation of agronomic and fruit quality traits of miraculin transgenic tomato. Biodiversitas 23: 2004-2009. Tomato cv. Moneymaker has been inserted with miraculin gene which can modify sour taste becomes sweet in the human tongue and, thus, it has potential as a sweetener. Evaluation of agronomic and fruit quality traits for this special transgenic tomato is a major concern that should be performed as substantial equivalence. The study's objective was to compare agronomic and fruit quality traits of transgenic miraculin tomato with those of its wild-type. The experiment employed a randomized block design, one genotype factor with two levels (transgenic vs. non-transgenic tomato cv. Moneymaker), and each was four replicates. Data were subjected to the Student t-test. The study revealed no significant differences between the transgenic tomato plants cv. Moneymaker and the wild type for important agronomic traits such as plant height, stem diameter, leaf area index, fruit weight, fruit weight per cluster, fruit weight per plant, fruit diameter, number of fruits per cluster, number of fruits per plant, and harvest index of miraculin. Some fruit quality traits such as fruit shelf-life, fruit hardness, total soluble solids, and content of glucose, sucrose, and fructose were equivalent to those of its counterpart, suggesting that miraculin transgene did not affect the expression of the observed traits. The miraculin transgenic tomato traits were more likely identical to those of wild-type except for the existence of the miraculin transgene. This will open the possibility for this special transgenic tomato to undergo further environmental safety assessment in the field test facility as a part of the biosafety assessment of genetically modified organism (GMO).

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...