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MIKSUSANTI MIKSUSANTI
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya. Jl. Palembang-Prabumulih Km. 32, Indralaya, Ogan Ilir 30662, South Sumatra, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Diversity of cellulolytic bacteria from Macrotermes gilvus gut isolated from Indralaya peatland region, Indonesia DWITA OKTIARNI; GETARI KASMIARTI; ERWIN NOFYAN; MIKSUSANTI MIKSUSANTI; HASANUDIN HASANUDIN; HERMANSYAH HERMANSYAH
Biodiversitas Journal of Biological Diversity Vol. 23 No. 1 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230152

Abstract

Abstract. Oktiarni D, Kasmiarti G, Nofyan E, Miksusanti, Hasanudin, Hermansyah. 2021. Diversity of cellulolytic bacteria from Macrotermes gilvus gut isolated from Indralaya peatland region, Indonesia. Biodiversitas 23: 486-495. The termite Macrotermes gilvus Hagen from Tanjung Senai, Inderalaya peatland region, is classified as a high-level termite. Termites like eusocial insects break down cellulosic biomass into glucose by bacteria that produce cellulolytic enzymes in their gut. In the present study, new species of cellulolytic bacteria from Macrotermes gilvus Hagen gut were determined by biomolecular assay. Bacterial isolates were isolated and purified by using DNA kit and the optical density of DNA bacterial isolates was obtained by using spectrophotometer nanodrop. A total of 24 bacterial isolates were amplified using PCR to determine the fragment of 16S rRNA gene, performed using BLAST-N program and compared with NCBI database. Result from the sequences of 16S rRNA gene showed that the new species were identify as Enterobacter cloacae (isolates 1, 3, 6, 7, 10, 14, 19, 20, 25, 28, 39, 40, 44, 60, and 64), Klebsiella pneumoniae (isolates 24, 43, and 62), Klebsiella quasipneumoniae (isolates 29, 36, and 37), Klebsiella varicolla (isolate 52), Enterobacter roggenkampii (isolate 56) and Enterobacter asburiae (isolate 59).
Short Communication: The isolation of Klebsiella variicola’s cellulase from Macrotermes gilvus gut in Indralaya Peatlands, Indonesia DWITA OKTIARNI; HERMANSYAH HERMANSYAH; EDDY IBRAHIM; MARSI MARSI; HASANUDIN HASANUDIN; MIKSUSANTI MIKSUSANTI; DEDE HERI YULI YANTO; NANIK RAHMANI; GETARI KASMIARTI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 8 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240802

Abstract

Abstract. Oktiarni D, Hermansyah, Ibrahim E, Marsi, Hasanudin, Miksusanti, Yanto DHY, Rahmani N, Kasmiarti G. 2023. Short Communication: The isolation of Klebsiella variicola’s cellulase from Macrotermes gilvus gut in Indralaya Peatlands, Indonesia. Biodiversitas 24: 4218-4222. Termites are organisms that can inflict damage on agricultural, forest, and furniture materials. Furthermore, they can positively affect the ecosystem due to their ability to break down lignocellulose-containing materials. According to previous findings, the cellulolytic bacteria from the termite Macrotermes gilvus gut obtained in Indralaya Peatlands showed activity and hydrolyzed cellulose in a CMC agar medium. In this study, the cellulase enzymes of Klebsiella variicola isolated from Macrotermes gilvus gut showed higher cellulolytic indexes after staining with Congo red. Furthermore, the enzyme was purified and characterized using ammonium sulfate precipitation and dialysis. SDS PAGE and zymogram analysis reported a molecular mass of 20 kDa, with optimal activity at pH of 6 and temperature of 50°C.
Co-Authors DEDE HERI YULI YANTO DWITA OKTIARNI DWITA OKTIARNI EDDY IBRAHIM ERWIN NOFYAN GETARI KASMIARTI GETARI KASMIARTI HASANUDIN HASANUDIN HERMANSYAH HERMANSYAH MARSI MARSI NANIK RAHMANI