Short Communication: The isolation of Klebsiella variicola’s cellulase from Macrotermes gilvus gut in Indralaya Peatlands, Indonesia

DWITA OKTIARNI (Doctoral Program of Mathematics and Natural Sciences, Graduate Program, Universitas Sriwijaya. Jl. Palembang-Prabumulih Km. 32, Indralaya, Ogan Ilir 30662, South Sumatra, Indonesia)
HERMANSYAH HERMANSYAH (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya. Jl. Palembang-Prabumulih Km. 32, Indralaya, Ogan Ilir 30662, South Sumatra, Indonesia)
EDDY IBRAHIM (Department of Mine, Faculty of Engineering, Universitas Sriwijaya. Jl. Raya Palembang-Prabumulih Km. 32, Indralaya, Ogan Ilir 30662, South Sumatra, Indonesia)
MARSI MARSI (Department of Soil, Faculty of Agriculture, Universitas Sriwijaya. Jl. Raya Palembang-Prabumulih Km. 32, Indralaya, Ogan Ilir 30662, South Sumatra, Indonesia)
HASANUDIN HASANUDIN (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya. Jl. Palembang-Prabumulih Km. 32, Indralaya, Ogan Ilir 30662, South Sumatra, Indonesia)
MIKSUSANTI MIKSUSANTI (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya. Jl. Palembang-Prabumulih Km. 32, Indralaya, Ogan Ilir 30662, South Sumatra, Indonesia)
DEDE HERI YULI YANTO (Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Cibinong Science Center. Jl. Raya Bogor Km. 46 Cibinong 16911, West Java, Indonesia)
NANIK RAHMANI (Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Cibinong Science Center. Jl. Raya Bogor Km. 46 Cibinong 16911, West Java, Indonesia)
GETARI KASMIARTI (Doctoral Program of Environmental Science, Graduate Program, Universitas Sriwijaya. Jl. Padang Selasa No.524, Bukit Besar, Palembang 30139, South Sumatra, Indonesia)

Biodiversitas Journal of Biological Diversity
Vol. 24 No. 8 (2023)

Abstract. Oktiarni D, Hermansyah, Ibrahim E, Marsi, Hasanudin, Miksusanti, Yanto DHY, Rahmani N, Kasmiarti G. 2023. Short Communication: The isolation of Klebsiella variicola’s cellulase from Macrotermes gilvus gut in Indralaya Peatlands, Indonesia. Biodiversitas 24: 4218-4222. Termites are organisms that can inflict damage on agricultural, forest, and furniture materials. Furthermore, they can positively affect the ecosystem due to their ability to break down lignocellulose-containing materials. According to previous findings, the cellulolytic bacteria from the termite Macrotermes gilvus gut obtained in Indralaya Peatlands showed activity and hydrolyzed cellulose in a CMC agar medium. In this study, the cellulase enzymes of Klebsiella variicola isolated from Macrotermes gilvus gut showed higher cellulolytic indexes after staining with Congo red. Furthermore, the enzyme was purified and characterized using ammonium sulfate precipitation and dialysis. SDS PAGE and zymogram analysis reported a molecular mass of 20 kDa, with optimal activity at pH of 6 and temperature of 50°C.