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All Journal Biodiversitas Journal of Biological Diversity
LENNI FITRI
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala. Jl. Syech Abdurrauf No. 3, Kopelma Darussalam, Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Toxicity test of rose periwinkle (Catharanthus roseus) leaves endophytic bacteria using Brine Shrimp Lethality Test (BSLT) method FAUZIAH FAUZIAH; MAULINASARI MAULINASARI; ESSY HARNELLY; YULIA SARI ISMAIL; LENNI FITRI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 1 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230122

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Abstract. Fauziah F, Maulinasari, Harnelly E, Ismail YS, Fitri L. 2021. Toxicity test of rose periwinkle (Catharanthus roseus) leaves endophytic bacteria using Brine Shrimp Lethality Test (BSLT) method. Biodiversitas 23: 171-177. Endophytic bacteria can be obtained from plants and may have the same ability as their host to produce secondary metabolites. This study aimed to determine the toxicity of endophytic bacterial extract from rose periwinkle (Catharanthus roseus) leaves using the Brine Shrimp Lethality Test (BSLT) method to determine their secondary metabolite contents. A toxicity test was carried out using Artemia salina shrimp larvae. The effect of the extract was determined based on the shrimp larvae mortality percentage using four concentration levels of 1000 ppm, 750 ppm, 500 ppm, and 250 ppm. Furthermore, the LC50 value of the extract against shrimp larvae was analyzed using SPSS software to obtain eight isolates of endophytic bacteria from Catharanthus roseus leaves. Macroscopic observations showed different characters, and five isolates were Gram-positive bacteria, while three were Gram-negative bacteria. The BETD5 isolate had the lowest LC50 value of 413,590 ppm, and the secondary metabolites of BETD5 extract were alkaloid and saponin. The molecular identification indicated that BETD5 had the highest identity percentage of 99.38% with Staphylococcus arlettae strain NR_036903, Staphylococcus gallinarum strain LMG 19119, Staphylococcus sp. strain Fop 222, Staphylococcus gallinarum strain E2 and Staphylococcus sp. strain MFC2.
Characterization and screening of urease activity of ureolytic bacteria from landfills soil in Banda Aceh, Indonesia LENNI FITRI; TEUKU BUDI AULIA; AMIR FAUZI; GALIH AHMAD KAMIL
Biodiversitas Journal of Biological Diversity Vol. 24 No. 2 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240229

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Abstract. Fitri L, Aulia TB, Fauzi A, Kamil GA. 2023. Characterization and screening of urease activity of ureolytic bacteria from landfills soil in Banda Aceh, Indonesia. Biodiversitas 24: 910-915. Ureolytic bacteria are capable of producing the calcium carbonate precipitating enzyme urease. Ureolytic bacteria degrade urease into ammonia and carbon dioxide. Ureolytic bacteria can be applied in the bio-grouting technique and concrete mixtures. This study aimed to isolate and characterize ureolytic bacteria isolates, then determine the calcium carbonate precipitation potential of ureolytic bacteria isolates from landfill soil at Gampong Jawa, Banda Aceh, Indonesia. This research successfully isolated twenty-four bacterial isolates from the Gampong Jawa landfills, and ten of these isolates were confirmed to produce the urease enzyme positively. Isolates with codes BTPA-3, BTPA-6, BTPA-7, BTPA-8, BTPA-9, BTPA-15, BTPA-20, BTPA-22, BTPA-23, and BTPA-24 were able to precipitate calcium carbonate in the amounts of 1.49, 1.78, 1.71, 1.70, 1.82, 1.80, 1.32, 1.54, and 1.70 g, respectively. BTPA-3, BTPA-6, BTPA-7, BTPA-8, BTPA-9, BTPA-23, and BTPA-24 were identified as members of the genus Bacillus; BTPA-20 was as a member of the genus Staphylococcus; and BTPA-15 and BTPA-22 were members of the genus Solibacillus. This research data is new information about the potential of bacteria from the Gampong Jawa landfill, which can determine the calcium carbonate precipitation. The research also revealed that the ureolytic bacterial isolates produced could be further improved and utilized in concrete mixtures.
Characterization of thermophilic bacteria from Ie Seum Hot Springs, Aceh Besar, Indonesia as producers of protease enzyme ELITA SABARIA; YEKKI YASMIN; YULIA S. ISMAIL; MOHAMMAD A. BESSANIA; ISNAINI PUTRI; LENNI FITRI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 5 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250502

Abstract

Abstract. Sabaria E, Yasmin Y, Ismail YS, Bessania MA, Putri I, Fitri L. 2024. Characterization of thermophilic bacteria from Ie Seum Hot Springs, Aceh Besar, Indonesia as producers of protease enzyme. Biodiversitas 25: 1867-1874. Thermophilic bacteria are microorganisms that can survive in high-temperature environments exceeding 75°C. The Ie Seum Hot Springs in Aceh Besar, Indonesia are known to be a place where these thermophilic bacteria inhabit. In such extreme conditions, proteins and enzymes are often denatured, making these bacteria of interest due to their ability to adapt and produce enzymes such as proteases. Proteases are called proteolytic enzymes that can be applied both in the economic and medical fields. This study aimed to isolate and identify thermophilic bacteria species that have the highest potential for protease production in Ie Seum Hot Spring, Aceh Besar by analyzing the 16S rRNA gene and characterizing them using biochemical tests. Based on the result, seven thermophilic bacterial isolates, namely BT1, BT2, BT3, BT4, BT5, BT6, and BT7 were obtained, each showing different colony characteristics. The biochemical tests also revealed differences metabolic activity of each isolate. Of these isolates, BT4 exhibited the highest proteolytic index (4.65). Additionally, the 16S rRNA gene sequence analysis showed that the BT4 strain belongs to the genus Bacillus and has high similarities to Bacillus sp., B. licheniformis and B. sonorensis. These findings suggest that BT4 has significant potential as a source of thermophilic protease enzymes.
Co-Authors AMIR FAUZI ELITA SABARIA ESSY HARNELLY FAUZIAH FAUZIAH GALIH AHMAD KAMIL ISNAINI PUTRI MAULINASARI MAULINASARI MOHAMMAD A. BESSANIA TEUKU BUDI AULIA YEKKI YASMIN YULIA S. ISMAIL YULIA SARI ISMAIL