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All Journal Biodiversitas Journal of Biological Diversity
NABELLA SURAYA
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Riau. Jl. Subrantas Km. 12,5, Kampus Bina Widya, Pekanbaru 28293, Riau, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Molecular identification of cellulase-producing thermophilic fungi isolated from Sungai Pinang hot spring, Riau Province, Indonesia SARYONO SARYONO; RIRYN NOVIANTY; NABELLA SURAYA; FINNA PISKA; SILVERA DEVI; NOVA WAHYU PRATIWI; AULIA ARDHI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 3 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230333

Abstract

Abstract. Saryono, Novianty R, Suraya N, Piska F, Devi S, Pratiwi NW, Ardhi A. 2022. Molecular identification of cellulase-producing thermophilic fungi isolated from Sungai Pinang hot spring, Riau Province, Indonesia. Biodiversitas 23: 1457-1465. Thermostable cellulolytic enzymes have become a subject of interest in industrial processes due to their ability to degrade cellulosic polysaccharides at elevated temperatures produced by microorganisms such as fungi and bacteria. In the present study, cellulase-producing thermophilic fungi were isolated and identified from Sungai Pinang hot spring, Riau Province, Indonesia. Morphological identification was carried out by macroscopic and microscopic observations. The ability of the thermophilic fungi to produce cellulase was determined using the clear zone test on carboxymethyl cellulose medium with a congo red staining. Isolate with the highest activity was identified molecularly using universal primers ITS1F and ITS4R. The results showed 19 isolates were morphologically identified as Aspergillus sp. and Penicillium sp. Based on qualitative testing, 3 of the19 isolates showed cellulase activity. The isolate performing the most considerable cellulase was LBKURCC293, which was identified as Aspergillus fumigatus with a cellulase activity of 2.6 x 10-2 IU/mL and a specific cellulase activity 8.0 x 10-3 IU/mg protein after 96 days of incubation.
Amylase enzyme production with variation of carbon sources and molecular identification of thermophilic fungus Aspergillus sp. LBKURCC304 from Bukik Gadang, West Sumatra, Indonesia SARYONO SARYONO; SILVERA DEVI; TITANIA T. NUGROHO; WIDYLIA FITRI FADHILA; LORENA LORENITA; FIDA SELFIANA NASUTION; NABELLA SURAYA
Biodiversitas Journal of Biological Diversity Vol. 24 No. 2 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240261

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Abstract. Saryono, Devi S, Nugroho TT, Fadhila WF, Lorenita L, Nasution FS, Suraya N. 2023. Amylase enzyme production with variation of carbon sources and molecular identification of thermophilic fungus Aspergillus sp. LBKURCC304 from Bukik Gadang, West Sumatra, Indonesia. Biodiversitas 24: 1200-1205. Amylase is an enzyme used to hydrolyze starch into smaller molecules. Starch degradation is very difficult because of the presence of 1-4 glucoside bonds from complex polysaccharides and the presence of enzyme accommodation centers, so the production of amylase is in great demand by industry. The production of amylase is strongly influenced by carbohydrates which act as inducers to stimulate the production of enzymes. This research was conducted to determine the effect of different carbohydrate sources on the production of amylase enzyme from thermophilic fungus Aspergillussp. LBKURCC304. Different carbon sources used werecassava, corn, taro, purple sweet potato, potato, breadfruit, canna, gembili, gadung, and sago. The effect of different carbohydrate sources on enzyme production was statistically tested using Duncan's Multiple Range Test (DMNRT) at a significant level of 5% and Principal Component Analysis (PCA). The results of molecular identification showed that carbohydrates from sago were a relatively better carbon source than other carbon sources, with an activity of 0.0391±0.0017 U/mL, anda specific activity of 0.0874±0.0049 U/mg. The highest (0.7651±0.0096 mg/mL) protein content wasrecorded from canna. Molecular identification showed that LBKURCC304 isolate was Aspergillus fumigatus.
Isolation and identification of lactic acid bacteria from traditional food sarobuong of Kuantan Singingi District, Riau, Indonesia SARYONO SARYONO; ISMAWATI ISMAWATI; NOVA WAHYU PRATIWI; SILVERA DEVI; MERRY YOHANA SIPAYUNG; NABELLA SURAYA
Biodiversitas Journal of Biological Diversity Vol. 24 No. 4 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240432

Abstract

Abstract. Saryono, Ismawati, Pratiwi NW, Devi S, Sipayung MY, Suraya N. 2023. Isolation and identification of lactic acid bacteria from traditional food sarobuong of Kuantan Singingi District, Riau, Indonesia. Biodiversitas 24: 2201-2206. Lactic acid bacteria (LAB) are commonly used as probiotic agents in fermented processed foods like sarobuong. Sarobuong is a traditional food from Kuantan Singingi District made by fermenting bamboo shoots. In this study, the isolation and purification of LAB from sarobuang were done using MRS agar media with the streak quadrant method: macroscopic and microscopic observations and biochemical and physiological tests to identify isolates. The antibacterial ability of LAB isolates was used against pathogenic bacteria Escherichia coli and Staphylococcus aureus. The results showed that 21 isolates were gram-positive bacteria in the form of bacilli and coccus, while carbohydrate degradation was homofermentative. The results of the antibacterial test of 21 isolates indicated by LAB against E. coli and S. aureus bacteria showed a clear zone ranging from 0.1 to 0.6. The highest antibacterial activity against pathogenic bacteria E. coli was observed in isolate RB1, while for pathogenic bacteria S. aureus, it was in isolate R15. The results obtained from several isolates that have been tested showed that LAB isolated from sarobuong had relatively higher activity than the control and was identified as Lactobacillus and Streptococcus.
Co-Authors AULIA ARDHI FIDA SELFIANA NASUTION FINNA PISKA ISMAWATI ISMAWATI LORENA LORENITA MERRY YOHANA SIPAYUNG NOVA WAHYU PRATIWI NOVA WAHYU PRATIWI RIRYN NOVIANTY SARYONO SARYONO SILVERA DEVI TITANIA T. NUGROHO WIDYLIA FITRI FADHILA