DUONG NGUYEN XUAN LAM
Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City. No. 217, Hong Bang Street, Ward 11, District 5, Ho Chi Minh City 700000, Vietnam

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Morphological characters, pharmacognostical parameters, and preliminary phytochemical screening of Curcuma sahuynhensis Škorni?k. & N.S.Lý in Quang Ngai Province, Vietnam TRAN VAN CHEN; DUONG NGUYEN XUAN LAM; CAO LY TAN THONG; DANH DUC NGUYEN; NGUYEN THANH TO NHI; NGUYEN THANH TRIET
Biodiversitas Journal of Biological Diversity Vol. 23 No. 8 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230807

Abstract

Abstract. Chen TV, Lam DNX, Thong CLT, Nguyen DD, Nhi NTT, Triet NT. 2022. Morphological characters, pharmacognostical parameters, and preliminary phytochemical screening of Curcuma sahuynhensis Škorni?k. & N.S.Lý in Quang Ngai Province, Vietnam. Biodiversitas 23: 3907-3920. Curcuma sahuynhensis (Vegetable Turmeric or Rau Nghe) has been used as a flavor and a spice in Vietnamese cuisines. Information on micromorphological characters, pharmacognostic parameters, and phytochemical screening of C. sahuynhensis has not been reported in the literature. Therefore, the accurate verification of plants requires further research to determine their quality and safety in order to make optimal use of them as raw materials for medicines and food. In this work, macro-and micromorphological features, pharmacognostic parameters, and phytochemical screening were performed according to the Vietnamese Pharmacopoeia V guidelines and other published data. Macromorphologically, it can grow up to 75 ± 5 cm tall and up to 10 per pseudostem. The inflorescence consists of 10–24 bracts. Rhizome branched, and remote tubers were present. The flower was yellow with a warm yellow midrib band, and an incision up to 0.6 cm long on the apex labellum. Anthers are L-shaped, stalked with no knobs below the thecae. Micromorphologically, there are anatomical similarities between the species in the genus Curcuma, particularly in terms of leaves, roots, and rhizomes. Under the lower epidermis of the leaf midrib are small vascular bundles (8–10 bundles). The stomatal index (SI) was 8.97 in abaxial and 1.06 in adaxial of the leaf. Prismatic calcium oxalate crystals were found in the leaf but they were absent in the root. Starch grains were also found in leaf powder (11–12.5 µm) and rhizome powder (15–20 µm). Additionally, the presence of oleoresin (in leaves and rhizomes) and the absence of crystals in the cortex region of the rhizome was found to be significant features in identifying C. sahuynhensis. Phytochemical screening found that its rhizome and inflorescence contain essential oil, amino acids, flavonoids, tannins, coumarins, triterpenoids, polyuronides, and reducing compounds. Pharmacognostically, rhizome powders' moisture content, total ash value, and acid-insoluble ash value were 11.79 ± 0.20%, 7.32 ± 0.60%, and 0.24 ± 0.08%, respectively.
Micromorphology, physicochemical parameters, and phytochemical screening of Chloranthus flavus D.T. Liu & G. Chen (syn. C. nervosus Collett & Hemsl.) from Vietnam TRAN VAN CHEN; DUONG NGUYEN XUAN LAM; HOANG DAC KHAI; DUONG PHAN NGUYEN DUC; NGUYEN THI NGA; NGUYEN THI THU HIEN; TRAN THI THUY QUYNH
Biodiversitas Journal of Biological Diversity Vol. 25 No. 2 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250245

Abstract

Abstract. Chen TV, Lam DNX, Khai HD, Duc DPN, Nga NT, Hien NTT, Quynh TTT. 2024. Micromorphology, physicochemical parameters, and phytochemical screening of Chloranthus flavus D.T. Liu & G. Chen (syn. C. nervosus Collett & Hemsl.) from Vietnam. Biodiversitas 25: 846-858. Chloranthus flavus D.T. Liu & G. Chen (syn. C. nervosus Collett & Hemsl.) is a subshrub belonging to the family Chloranthaceae. Information on the anatomical structure and the powder features of C. flavus has not been noted. This study aimed to develop a monograph on the microscopic characteristics and physicochemical parameters of C. flavus, as well as a preliminary assessment of the phytochemical composition of this species. Anatomical characterizations of roots, stems, and leaves were performed using the iodine green-carmine staining method and then an optical microscope was used to observe the histology of these parts, similar to observing powder characteristics. Secondary metabolites were detected by chromogenic or precipitation reagents. Anatomically, the structural characteristics of the roots, stems, and leaves are similar to those of the Chloranthus species. Particularly, the root structure is characterized by six alternating xylem bundles, with six phloem bundles forming a ring. Polygonal to irregularly shaped cells of the upper epidermis and irregularly shaped ordinary cells, laterocytic and anomocytic stomata types, stomatal clusters, and the stomatal index (15.75±1.52%) of the lower epidermis were observed in leaves. Both powders found fragments of epidermal and parenchymatous cells, spiral xylem vessels, and scalariform xylem vessels. However, the root powder only observed solid-shaped sclereid cells, starch granules, starch clusters, and calcium oxalate crystals. The powders' moisture content, total ash, and acid-insoluble ash values all meet acceptable standards according to regulations on raw material purity. The whole C. flavus plant contains carotenoids, fatty acids, essential oils, flavonoids, tannins, coumarins, alkaloids, triterpenoids, amino acids, and carbohydrates. The current findings highlight the key characteristics for identifying C. flavus.